Validation of organ culture in colonic adaptation to surgical manipulation.
University Department of Surgery, Southmead Hospital, Bristol.
Valuable information on intestinal adaptation can be gained by using the technique of organ culture to measure the crypt cell production rate. It is not known, however, whether the production rate in organ culture accurately represent that in vivo. Colonic crypt cell production rate, determined by a standard method in vivo, was compared with that in vitro in organ culture in 56 rats. Extensive jejunoileal bypass was used to stimulate colonic hyperplasia, and colonic defunction (by transverse colostomy) led to hypoplasia. There were no differences in crypt cell production rates between in vivo and in vitro groups in normal colon (4.62 (0.39) v 4.80 (0.23) cells/crypt/hour), after 80% jejuno-ileal bypass (7.81 (0.71) v 6.75 (0.72) cells/crypt/hour), or after defunction (2.11 (0.39) v 1.81 (0.35) cells/crypt/hour). Adapting colonic mucosa does not undergo appreciable readaptation in vitro in short term organ culture (10-24 hours). Crypt cell production rate results obtained in man probably reflect in vivo values.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
Services
- Email this link to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Add article to my folders
- Download to citation manager
- Request Permissions
Citing Articles
Google Scholar
PubMed
Topic Collections
Bookmark with
Register for free content
The full back archive is now available for all BMJ Journals. Institutional subscribers may access the entire archive as part of their subscription. Personal subscribers will also have access to all content when logged in. Non-subscribers who register have free access to all articles published before 2006 right back to volume 1 issue 1. Register here to access the free archive of all BMJ Journals.
Don't forget to sign up for content alerts so you keep up to date with all the articles as they are published.
