Lactobacillus acidophilus LA 1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria.

M F Bernet, D Brassart, J R Neeser, A L Servin

Département de Microbiologie, UFR Sciences Pharmaceutiques, Paris, France.

Four human Lactobacillus acidophilus strains were tested for their ability to adhere onto human enterocyte like Caco-2 cells in culture. The LA 1 strain exhibited a high calcium independent adhesive property. This adhesion onto Caco-2 cells required a proteinaceous adhesion promoting factor, which was present in the spent bacterial broth culture supernatant. LA 1 strain also strongly bound to the mucus secreted by the homogeneous cultured human goblet cell line HT29-MTX. The inhibitory effect of LA 1 organisms against Caco-2 cell adhesion and cell invasion by a large variety of diarrhoeagenic bacteria was investigated. As a result, the following dose dependent inhibitions were obtained: (a) against the cell association of enterotoxigenic, diffusely adhering and enteropathogenic Escherichia coli, and Salmonella typhimurium; (b) against the cell invasion by enteropathogenic Escherichia coli, Yersinia pseudotuberculosis, and Salmonella typhimurium. Incubations of L acidophilus LA 1 before and together with enterovirulent E coli were more effective than incubation after infection by E coli.

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• Isakow, W., Morrow, L. E., Kollef, M. H. (2007). Probiotics for Preventing and Treating Nosocomial Infections: Review of Current Evidence and Recommendations. Chest 132: 286-294 [Abstract] [Full Text]  
• Pfeiler, E. A., Azcarate-Peril, M. A., Klaenhammer, T. R. (2007). Characterization of a Novel Bile-Inducible Operon Encoding a Two-Component Regulatory System in Lactobacillus acidophilus. J. Bacteriol. 189: 4624-4634 [Abstract] [Full Text]  
• Lesbros-Pantoflickova, D., Corthesy-Theulaz, I., Blum, A. L. (2007). Helicobacter pylori and Probiotics. J. Nutr. 137: 812S-818S [Abstract] [Full Text]  
• Bai, A-P, Ouyang, Q (2006). Probiotics and inflammatory bowel diseases.. Postgrad. Med. J. 82: 376-382 [Abstract] [Full Text]  
• Liong, M. T., Shah, N. P. (2006). Effects of a Lactobacillus casei Synbiotic on Serum Lipoprotein, Intestinal Microflora, and Organic Acids in Rats. J DAIRY SCI 89: 1390-1399 [Abstract] [Full Text]  
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• Buck, B. L., Altermann, E., Svingerud, T., Klaenhammer, T. R. (2005). Functional Analysis of Putative Adhesion Factors in Lactobacillus acidophilus NCFM. Appl. Environ. Microbiol. 71: 8344-8351 [Abstract] [Full Text]  
• Haza, A. I., Zabala, A., Arranz, N., Morales, P. (2005). Protective Effect of a Lactobacillus salivarius Strain of Human Origin. Food Science and Technology International 11: 251-259 [Abstract]  
• Gionchetti, P, Lammers, K M, Rizzello, F, Campieri, M (2005). Probiotics and barrier function in colitis. Gut 54: 898-900 [Full Text]  
• Llopis, M, Antolin, M, Guarner, F, Salas, A, Malagelada, J-R (2005). Mucosal colonisation with Lactobacillus casei mitigates barrier injury induced by exposure to trinitronbenzene sulphonic acid. Gut 54: 955-959 [Abstract] [Full Text]  
• Ingrassia, I., Leplingard, A., Darfeuille-Michaud, A. (2005). Lactobacillus casei DN-114 001 Inhibits the Ability of Adherent-Invasive Escherichia coli Isolated from Crohn's Disease Patients To Adhere to and To Invade Intestinal Epithelial Cells. Appl. Environ. Microbiol. 71: 2880-2887 [Abstract] [Full Text]  
• Stecher, B., Macpherson, A. J., Hapfelmeier, S., Kremer, M., Stallmach, T., Hardt, W.-D. (2005). Comparison of Salmonella enterica Serovar Typhimurium Colitis in Germfree Mice and Mice Pretreated with Streptomycin. Infect. Immun. 73: 3228-3241 [Abstract] [Full Text]  
• Benyacoub, J., Perez, P. F., Rochat, F., Saudan, K. Y., Reuteler, G., Antille, N., Humen, M., De Antoni, G. L., Cavadini, C., Blum, S., Schiffrin, E. J. (2005). Enterococcus faecium SF68 Enhances the Immune Response to Giardia intestinalis in Mice. J. Nutr. 135: 1171-1176 [Abstract] [Full Text]  
• Martin, R., Olivares, M., Marin, M. L., Fernandez, L., Xaus, J., Rodriguez, J. M. (2005). Probiotic Potential of 3 Lactobacilli Strains Isolated From Breast Milk. J Hum Lact 21: 8-17 [Abstract]  
• Humen, M. A., De Antoni, G. L., Benyacoub, J., Costas, M. E., Cardozo, M. I., Kozubsky, L., Saudan, K.-Y., Boenzli-Bruand, A., Blum, S., Schiffrin, E. J., Perez, P. F. (2005). Lactobacillus johnsonii La1 Antagonizes Giardia intestinalis In Vivo. Infect. Immun. 73: 1265-1269 [Abstract] [Full Text]  
• Braat, H., van den Brande, J., van Tol, E., Hommes, D., Peppelenbosch, M., van Deventer, S. (2004). Lactobacillus rhamnosus induces peripheral hyporesponsiveness in stimulated CD4+ T cells via modulation of dendritic cell function. Am. J. Clin. Nutr. 80: 1618-1625 [Abstract] [Full Text]  
• Adolfsson, O., Meydani, S. N., Russell, R. M (2004). Yogurt and gut function. Am. J. Clin. Nutr. 80: 245-256 [Abstract] [Full Text]  
• Granato, D., Bergonzelli, G. E., Pridmore, R. D., Marvin, L., Rouvet, M., Corthesy-Theulaz, I. E. (2004). Cell Surface-Associated Elongation Factor Tu Mediates the Attachment of Lactobacillus johnsonii NCC533 (La1) to Human Intestinal Cells and Mucins. Infect. Immun. 72: 2160-2169 [Abstract] [Full Text]  
• Pridmore, R. D., Berger, B., Desiere, F., Vilanova, D., Barretto, C., Pittet, A.-C., Zwahlen, M.-C., Rouvet, M., Altermann, E., Barrangou, R., Mollet, B., Mercenier, A., Klaenhammer, T., Arigoni, F., Schell, M. A. (2004). The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533. Proc. Natl. Acad. Sci. USA 101: 2512-2517 [Abstract] [Full Text]  
• Anderson, A D G, McNaught, C E, Jain, P K, MacFie, J (2004). Randomised clinical trial of synbiotic therapy in elective surgical patients. Gut 53: 241-245 [Abstract] [Full Text]  
• Zsivkovits, M., Fekadu, K., Sontag, G., Nabinger, U., Huber, W. W., Kundi, M., Chakraborty, A., Foissy, H., Knasmuller, S. (2003). Prevention of heterocyclic amine-induced DNA damage in colon and liver of rats by different lactobacillus strains. Carcinogenesis 24: 1913-1918 [Abstract] [Full Text]  
• Reid, G., Jass, J., Sebulsky, M. T., McCormick, J. K. (2003). Potential Uses of Probiotics in Clinical Practice. Clin. Microbiol. Rev. 16: 658-672 [Abstract] [Full Text]  
• Ibnou-Zekri, N., Blum, S., Schiffrin, E. J., von der Weid, T. (2003). Divergent Patterns of Colonization and Immune Response Elicited from Two Intestinal Lactobacillus Strains That Display Similar Properties In Vitro. Infect. Immun. 71: 428-436 [Abstract] [Full Text]  
• Yan, F., Polk, D. B. (2002). Probiotic Bacterium Prevents Cytokine-induced Apoptosis in Intestinal Epithelial Cells. J. Biol. Chem. 277: 50959-50965 [Abstract] [Full Text]  
• McNaught, C E, Woodcock, N P, MacFie, J, Mitchell, C J (2002). A prospective randomised study of the probiotic Lactobacillus plantarum 299V on indices of gut barrier function in elective surgical patients. Gut 51: 827-831 [Abstract] [Full Text]  
• Nam, H., Ha, M., Bae, O., Lee, Y. (2002). Effect of Weissella confusa Strain PL9001 on the Adherence and Growth of Helicobacter pylori. Appl. Environ. Microbiol. 68: 4642-4645 [Abstract] [Full Text]  
• Haller, D., Serrant, P., Granato, D., Schiffrin, E. J., Blum, S. (2002). Activation of Human NK Cells by Staphylococci and Lactobacilli Requires Cell Contact-Dependent Costimulation by Autologous Monocytes. CVI 9: 649-657 [Abstract] [Full Text]  
• Duggan, C., Gannon, J., Walker, W A. (2002). Protective nutrients and functional foods for the gastrointestinal tract. Am. J. Clin. Nutr. 75: 789-808 [Abstract] [Full Text]  
• Swanson, K. S., Grieshop, C. M., Flickinger, E. A., Bauer, L. L., Healy, H.-P., Dawson, K. A., Merchen, N. R., Fahey, G. C. Jr (2002). Supplemental Fructooligosaccharides and Mannanoligosaccharides Influence Immune Function, Ileal and Total Tract Nutrient Digestibilities, Microbial Populations and Concentrations of Protein Catabolites in the Large Bowel of Dogs. J. Nutr. 132: 980-989 [Abstract] [Full Text]  
• Perez, P. F., Minnaard, J., Rouvet, M., Knabenhans, C., Brassart, D., De Antoni, G. L., Schiffrin, E. J. (2001). Inhibition of Giardia intestinalis by Extracellular Factors from Lactobacilli: an In Vitro Study. Appl. Environ. Microbiol. 67: 5037-5042 [Abstract] [Full Text]  
• Hudault, S, Guignot, J, Servin, A L (2001). Escherichia coli strains colonising the gastrointestinal tract protect germfree mice against Salmonella typhimurium infection. Gut 49: 47-55 [Abstract] [Full Text]  
• Lu, L., Walker, W A. (2001). Pathologic and physiologic interactions of bacteria with the gastrointestinal epithelium. Am. J. Clin. Nutr. 73 : 1124S-1130S [Abstract] [Full Text]  
• Dunne, C., O'Mahony, L., Murphy, L., Thornton, G., Morrissey, D., O'Halloran, S., Feeney, M., Flynn, S., Fitzgerald, G., Daly, C., Kiely, B., O'Sullivan, G. C, Shanahan, F., Collins, J K. (2001). In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. Am. J. Clin. Nutr. 73: 386S-392 [Abstract] [Full Text]  
• Verschuere, L., Rombaut, G., Sorgeloos, P., Verstraete, W. (2000). Probiotic Bacteria as Biological Control Agents in Aquaculture. Microbiol. Mol. Biol. Rev. 64: 655-671 [Abstract] [Full Text]  
• Bruce, W. R., Giacca, A., Medline, A. (2000). Possible Mechanisms Relating Diet and Risk of Colon Cancer. Cancer Epidemiol. Biomarkers Prev. 9: 1271-1279 [Abstract] [Full Text]  
• Lievin, V, Peiffer, I, Hudault, S, Rochat, F, Brassart, D, Neeser, J-R, Servin, A L (2000). Bifidobacterium strains from resident infant human gastrointestinal microflora exert antimicrobial activity. Gut 47: 646-652 [Abstract] [Full Text]  
• Neeser, J.-R., Granato, D., Rouvet, M., Servin, A., Teneberg, S., Karlsson, K.-A. (2000). Lactobacillus johnsonii La1 shares carbohydrate-binding specificities with several enteropathogenic bacteria. Glycobiology 10: 1193-1199 [Abstract] [Full Text]  
• Jin, L. Z., Marquardt, R. R., Zhao, X. (2000). A Strain of Enterococcus faecium (18C23) Inhibits Adhesion of Enterotoxigenic Escherichia coli K88 to Porcine Small Intestine Mucus. Appl. Environ. Microbiol. 66: 4200-4204 [Abstract] [Full Text]  
• Lee, Y. K., Lim, C. Y., Teng, W. L., Ouwehand, A. C., Tuomola, E. M., Salminen, S. (2000). Quantitative Approach in the Study of Adhesion of Lactic Acid Bacteria to Intestinal Cells and Their Competition with Enterobacteria. Appl. Environ. Microbiol. 66: 3692-3697 [Abstract] [Full Text]  
• Haller, D, Bode, C, Hammes, W P, Pfeifer, A M A, Schiffrin, E J, Blum, S (2000). Non-pathogenic bacteria elicit a differential cytokine response by intestinal epithelial cell/leucocyte co-cultures. Gut 47: 79-87 [Abstract] [Full Text]  
• TAKAHASHI, M., TAGUCHI, H., YAMAGUCHI, H., OSAKI, T., KAMIYA, S. (2000). Studies of the effect of Clostridium butyricum on Helicobacter pylori in several test models including gnotobiotic mice. J Med Microbiol 49: 635-642 [Abstract] [Full Text]  
• Kennedy, R.J., Kirk, S.J., Gardiner, K.R. (2000). Promotion of a Favorable Gut Flora in Inflammatory Bowel Disease. JPEN J Parenter Enteral Nutr 24: 189-195 [Abstract]  
• Meydani, S. N., Ha, W.-K. (2000). Immunologic effects of yogurt. Am. J. Clin. Nutr. 71: 861-872 [Abstract] [Full Text]  
• Jacobsen, C. N., Rosenfeldt Nielsen, V., Hayford, A. E., Møller, P. L., Michaelsen, K. F., Pærregaard, A., Sandström, B., Tvede, M., Jakobsen, M. (1999). Screening of Probiotic Activities of Forty-Seven Strains of Lactobacillus spp. by In Vitro Techniques and Evaluation of the Colonization Ability of Five Selected Strains in Humans. Appl. Environ. Microbiol. 65: 4949-4956 [Abstract] [Full Text]  
• Reid, G. (1999). The Scientific Basis for Probiotic Strains of Lactobacillus. Appl. Environ. Microbiol. 65: 3763-3766 [Full Text]  
• Lapierre, L., Germond, J.-E., Ott, A., Delley, M., Mollet, B. (1999). D-Lactate Dehydrogenase Gene (ldhD) Inactivation and Resulting Metabolic Effects in the Lactobacillus johnsonii Strains La1 and N312. Appl. Environ. Microbiol. 65: 4002-4007 [Abstract] [Full Text]  
• Macfarlane, G. T, Cummings, J. H (1999). Probiotics and prebiotics: can regulating the activities of intestinal bacteria benefit health?. BMJ 318: 999-1003 [Full Text]  
• Mack, D. R., Michail, S., Wei, S., McDougall, L., Hollingsworth, M. A. (1999). Probiotics inhibit enteropathogenic E. coli adherence in vitro by inducing intestinal mucin gene expression. Am. J. Physiol. Gastrointest. Liver Physiol. 276: G941-G950 [Abstract] [Full Text]  
• Granato, D., Perotti, F., Masserey, I., Rouvet, M., Golliard, M., Servin, A., Brassart, D. (1999). Cell Surface-Associated Lipoteichoic Acid Acts as an Adhesion Factor for Attachment of Lactobacillus johnsonii La1 to Human Enterocyte-Like Caco-2 Cells. Appl. Environ. Microbiol. 65: 1071-1077 [Abstract] [Full Text]  
• Videla, S., García-Lafuente, A., Antolín, M., Vilaseca, J., Guarner, F., Crespo, E., González, G., Salas, A., Malagelada, J. R. (1998). Antitumor Necrosis Factor Therapy in Rat Chronic Granulomatous Colitis: Critical Dose-Timing Effects on Outcome. J. Pharmacol. Exp. Ther. 287: 854-859 [Abstract] [Full Text]  
• Coconnier, M.-H., Lievin, V., Hemery, E., Servin, A. L. (1998). Antagonistic Activity against Helicobacter Infection In Vitro and In Vivo by the Human Lactobacillus acidophilus Strain LB. Appl. Environ. Microbiol. 64: 4573-4580 [Abstract] [Full Text]  
• BENGMARK, S (1998). Ecological control of the gastrointestinal tract. The role of probiotic flora. Gut 42: 2-7 [Full Text]  
• Elmer, G. W., Surawicz, C. M., McFarland, L. V. (1996). Biotherapeutic Agents: A Neglected Modality for the Treatment and Prevention of Selected Intestinal and Vaginal Infections. JAMA 275: 870-876 [Abstract]  
• Katelaris, P. H., Salam, I., Farthing, M. J.G. (1995). Lactobacilli to Prevent Traveler's Diarrhea?. NEJM 333: 1360-1361 [Full Text]