K-ras gene mutation in gall bladder carcinomas and dysplasia.

T Ajiki, T Fujimori, H Onoyama, M Yamamoto, S Kitazawa, S Maeda and Y Saitoh

Second Department of Pathology, Kobe University School of Medicine, Japan.

Epithelial dysplasia of gall bladder is an important precancerous lesion of gall bladder carcinogenesis. To investigate the frequency of K-ras gene mutation in gall bladder carcinoma and dysplasia, K-ras codon 12 mutations were investigated by the polymerase chain reaction/restriction enzyme based method following direct sequencing. Mutation was detected in 59% (30 of 51) of gall bladder carcinomas, in 73% (8 of 11) of gall bladder dysplasia in gall stone cases, and in 0% of the normal gall bladder epithelium. There was, however, no correlation between K-ras mutation and clinicopathological factors of gall bladder carcinoma. K-ras gene mutation occurs even in gall bladder dysplasia at an incidence similar to that in carcinomas, suggesting that testing for K-ras gene mutation may prove useful as an adjunct to bile cytological or biopsy analysis.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Hori, H., Ajiki, T., Fujita, T., Okazaki, T., Suzuki, Y., Kuroda, Y., Fujimori, T. (2006). Double Cancer of Gall Bladder and Bile Duct not Associated with Anomalous Junction of the Pancreaticobiliary Duct System. Jpn J Clin Oncol 36: 638-642 [Abstract] [Full Text]  
• Takahashi, T., Shivapurkar, N., Riquelme, E., Shigematsu, H., Reddy, J., Suzuki, M., Miyajima, K., Zhou, X., Bekele, B. N., Gazdar, A. F., Wistuba, I. I. (2004). Aberrant Promoter Hypermethylation of Multiple Genes in Gallbladder Carcinoma and Chronic Cholecystitis. Clin. Cancer Res. 10: 6126-6133 [Abstract] [Full Text]  
• House, M. G., Wistuba, I. I., Argani, P., Guo, M., Schulick, R. D., Hruban, R. H., Herman, J. G., Maitra, A. (2003). Progression of Gene Hypermethylation in Gallstone Disease Leading to Gallbladder Cancer. Ann. Surg. Oncol. 10: 882-889 [Abstract] [Full Text]  
• Lazcano-Ponce, E. C., Miquel, J. F., Munoz, N., Herrero, R., Ferrecio, C., Wistuba, I. I., Alonso de Ruiz, P., Aristi Urista, G., Nervi, F. (2001). Epidemiology and Molecular Pathology of Gallbladder Cancer. CA Cancer J Clin 51: 349-364 [Abstract] [Full Text]  
• Kubicka, S, Kuhnel, F, Flemming, P, Hain, B, Kezmic, N, Rudolph, K L, Manns, M, Meier, P N (2001). K-ras mutations in the bile of patients with primary sclerosing cholangitis. Gut 48: 403-408 [Abstract] [Full Text]  
• Shi, Y.-Z., Hui, A.-M., Li, X., Takayama, T., Makuuchi, M. (2000). Overexpression of Retinoblastoma Protein Predicts Decreased Survival and Correlates with Loss of p16INK4 Protein in Gallbladder Carcinomas. Clin. Cancer Res. 6: 4096-4100 [Abstract] [Full Text]  
• Hidaka, E., Yanagisawa, A., Seki, M., Takano, K., Setoguchi, T., Kato, Y. (2000). High Frequency of K-ras Mutations in Biliary Duct Carcinomas of Cases with a Long Common Channel in the Papilla of Vater. Cancer Res. 60: 522-524 [Abstract] [Full Text]