Butyrate enema therapy stimulates mucosal repair in experimental colitis in the rat.

J D Butzner, R Parmar, C J Bell and V Dalal

Gastrointestinal Research Group, Faculty of Medicine, University of Calgary, Alberta, Canada.

BACKGROUND--The short chain fatty acid (SCFA) butyrate provides energy for colonocytes, stimulates colonic fluid and electrolyte absorption and is recognised as an effective treatment for multiple types of colitis. AIM--To examine the impact of butyrate enema therapy on the clinical course, severity of inflammation, and SCFA stimulated Na+ absorption in a chronic experimental colitis. METHODS--Distal colitis was induced in rats with a trinitrobenzenesulphonic acid (TNBS) enema. Five days after induction, rats were divided into groups to receive: no treatment, saline enemas, or 100 mM Na-butyrate enemas daily. On day 24, colonic damage score and tissue myeloperoxidase (MPO) activity were evaluated. Colon was mounted in Ussing chambers and Na+ transport and electrical activities were measured during a basal period and after stimulation with 25 mM butyrate. RESULTS--In the untreated and the saline enema treated TNBS groups, diarrhoea and extensive colonic damage were seen, associated with increased tissue MPO activities and absent butyrate stimulated Na+ absorption. In contrast, in the butyrate enema treated TNBS group, diarrhoea ceased, colonic damage score improved, and tissue MPO activity as well as butyrate stimulated Na+ absorption recovered to control values. CONCLUSION--Butyrate enema therapy stimulated colonic repair, as evidenced by clinical recovery, decreased inflammation, and restoration of SCFA stimulated electrolyte absorption.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Arvans, D. L., Vavricka, S. R., Ren, H., Musch, M. W., Kang, L., Rocha, F. G., Lucioni, A., Turner, J. R., Alverdy, J., Chang, E. B. (2005). Luminal bacterial flora determines physiological expression of intestinal epithelial cytoprotective heat shock proteins 25 and 72. Am. J. Physiol. Gastrointest. Liver Physiol. 288: G696-G704 [Abstract] [Full Text]  
• Klampfer, L., Huang, J., Sasazuki, T., Shirasawa, S., Augenlicht, L. (2003). Inhibition of Interferon {gamma} Signaling by the Short Chain Fatty Acid Butyrate. Mol Cancer Res 1: 855-862 [Abstract] [Full Text]  
• Venkatraman, A., Ramakrishna, B. S., Shaji, R. V., Kumar, N. S. N., Pulimood, A., Patra, S. (2003). Amelioration of dextran sulfate colitis by butyrate: role of heat shock protein 70 and NF-{kappa}B. Am. J. Physiol. Gastrointest. Liver Physiol. 285: G177-G184 [Abstract] [Full Text]  
• Cherbut, C., Michel, C., Lecannu, G. (2003). The Prebiotic Characteristics of Fructooligosaccharides Are Necessary for Reduction of TNBS-Induced Colitis in Rats. J. Nutr. 133: 21-27 [Abstract] [Full Text]  
• Segain, J-P, de la Bletiere, D R., Bourreille, A, Leray, V, Gervois, N, Rosales, C, Ferrier, L, Bonnet, C, Blottiere, H M, Galmiche, J-P (2000). Butyrate inhibits inflammatory responses through NFkappa B inhibition: implications for Crohn's disease. Gut 47: 397-403 [Abstract] [Full Text]  
• Barcelo, A, Claustre, J, Moro, F, Chayvialle, J-A, Cuber, J-C, Plaisancie, P (2000). Mucin secretion is modulated by luminal factors in the isolated vascularly perfused rat colon. Gut 46: 218-224 [Abstract] [Full Text]