Acute epithelial injury in the rat small intestine in vivo is associated with expanded expression of transforming growth factor alpha and beta.

A U Dignass, J L Stow, M W Babyatsky

Gastrointestinal Unit, Massachusetts General Hospital, Boston, USA.

BACKGROUND--Previous studies have shown the importance of transforming growth factors alpha and beta (TGF alpha and TGF beta) in modulating epithelial cell restitution after injury in vitro. AIM--To investigate the role of the growth factors TGF alpha and TGF beta after acute epithelial injury in vivo. METHODS--An in vivo model of phytohaemagglutinin (PHA) induced acute epithelial injury in rat small intestine was used. Epithelial cell turnover was assessed by autoradiography and liquid scintillation counting of thymidine uptake. Expression of TGF alpha and TGF beta was assessed by immunohistochemistry. RESULTS--An expansion of the proliferative compartment and increased turnover of intestinal epithelial cells was seen in rats with PHA induced intestinal epithelial injury. Expression of TGF alpha and TGF beta peptides was shown in both the epithelial cell and lamina propria compartment. Different patterns of TGF alpha and TGF beta expression were seen, however, within the epithelium of rats with acute intestinal injury compared with untreated controls, while the expression of these peptides within the lamina propria was not changed. CONCLUSIONS--These findings suggest that acute intestinal epithelial cell injury in vivo is associated with compensatory changes in expression of TGF alpha and TGF beta in the epithelial cell compartment, while the lamina propria does not seem to be significantly affected.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Belmiro, C. L. R., Castelo-Branco, M. T. L., Melim, L. M. C., Schanaider, A., Elia, C., Madi, K., Pavao, M. S. G., de Souza, H. S. P. (2009). Unfractionated Heparin and New Heparin Analogues from Ascidians (Chordate-Tunicate) Ameliorate Colitis in Rats. J. Biol. Chem. 284: 11267-11278 [Abstract] [Full Text]  
• O'Mahony, F., Toumi, F., Mroz, M. S., Ferguson, G., Keely, S. J. (2008). Induction of Na+/K+/2Cl- cotransporter expression mediates chronic potentiation of intestinal epithelial Cl- secretion by EGF. Am. J. Physiol. Cell Physiol. 294: C1362-C1370 [Abstract] [Full Text]  
• Zhou, S., Ueta, H., Xu, X.-D., Shi, C., Matsuno, K. (2008). Predominant donor CD103+CD8+ T cell infiltration into the gut epithelium during acute GvHD: a role of gut lymph nodes. Int Immunol 20: 385-394 [Abstract] [Full Text]  
• El-Asady, R., Yuan, R., Liu, K., Wang, D., Gress, R. E., Lucas, P. J., Drachenberg, C. B., Hadley, G. A. (2005). TGF-{beta}-dependent CD103 expression by CD8+ T cells promotes selective destruction of the host intestinal epithelium during graft-versus-host disease. JEM 201: 1647-1657 [Abstract] [Full Text]  
• Aymerich, I., Pastor-Anglada, M., Casado, F. J. (2004). Long Term Endocrine Regulation of Nucleoside Transporters in Rat Intestinal Epithelial Cells. JGP 124: 505-512 [Abstract] [Full Text]  
• Booth, B. W., Adler, K. B., Bonner, J. C., Tournier, F., Martin, L. D. (2001). Interleukin-13 Induces Proliferation of Human Airway Epithelial Cells In Vitro via a Mechanism Mediated by Transforming Growth Factor-alpha. Am. J. Respir. Cell Mol. Bio. 25: 739-743 [Abstract] [Full Text]  
• Powell, D. W., Mifflin, R. C., Valentich, J. D., Crowe, S. E., Saada, J. I., West, A. B. (1999). Myofibroblasts. II. Intestinal subepithelial myofibroblasts. Am. J. Physiol. Cell Physiol. 277: C183-C201 [Abstract] [Full Text]  
• Beltinger, J., Hawkey, C. J., Stack, W. A. (1999). TGF-alpha reduces bradykinin-stimulated ion transport and prostaglandin release in human colonic epithelial cells. Am. J. Physiol. Cell Physiol. 276: C848-C855 [Abstract] [Full Text]  
• Drucker, D. J., Deforest, L., Brubaker, P. L. (1997). Intestinal response to growth factors administered alone or in combination with human [Gly2]glucagon-like peptide 2. Am. J. Physiol. Gastrointest. Liver Physiol. 273: G1252-G1262 [Abstract] [Full Text]