GUT 1998;42:470-476 ( April )

Increased secretion of pro-inflammatory cytokines by circulating polymorphonuclear neutrophils and regulation by interleukin 10 during intestinal inflammation

S Nikolaus,^a J Bauditz,^a P Gionchetti,^b C Witt,^c H Lochs,^a S Schreiber^a

^a Charité University Hospital, 4th Medical Department, Humboldt University, Berlin, Germany, ^b Clinica Media I, Policlinico S. Orsola, University of Bologna, Italy, ^c Charité University Hospital, 1st Medical Department, Humboldt University, Berlin, Germany

Correspondence to: Dr S Schreiber, 1st Department of Medicine, Christian-Albrechts-University, Schittenhelmstrasse 12, 24105 Kiel, Germany (email: s.schreiber{at}mucosa.de).

Accepted for publication 31 October 1997

BackgroundConcentrations of pro-inflammatory cytokines are increased in the intestinal mucosa of patients with active inflammatory bowel disease (IBD). Polymorphonuclear neutrophil granulocytes (PMN) are the most abundant cell type in intestinal lesions in IBD. Interleukin 10 (IL-10) is an important contra-inflammatory cytokine which induces downregulation of pro-inflammatory cytokines.
AimsTo investigate whether PMN from patients with IBD or infectious colitis, respectively, secrete increased amounts of pro-inflammatory cytokines and can be regulated by IL-10.
MethodsSecretion (ELISA) as well as corresponding mRNA levels (semiquantitative RT-PCR) of pro-inflammatory cytokines (IL-1, TNF-) and of IL-1 receptor antagonist were assessed in peripheral PMN.
ResultsPMN from patients with IBD are primed to secrete enhanced amounts of pro-inflammatory cytokines accompanied by detection of corresponding mRNAs in comparison with normal controls. This finding is not specific for IBD but rather reflects intestinal inflammation in general. IL-10 markedly inhibited pro-inflammatory cytokine secretion as well as corresponding mRNA concentrations.
ConclusionsPMN are an important source of pro-inflammatory cytokines in patients with intestinal inflammation and can be downregulated by IL-10.
(GUT 1998;42:470-476)

Keywords: granulocytes;  interleukin 1;  interleukin 10;  inflammatory bowel disease;  intestinal immunity;  inflammation;  neutrophils;  tumour necrosis factor

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© 1998 by Gut
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