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J Carrère
a Groupe de Recherche
sur les Glandes Exocrines, Faculté de Médecine, 27, Boulevard Jean
Moulin, 13005 Marseille, France, b CERM, Hôpital Renée Sabran, Giens,
83406 Hyères, France, c Unità di Gastroenterologia, Ospedale S Luigi,
Turin, Italy
Correspondence to: Dr C
Figarella, Groupe de Recherche sur les Glandes Exocrines, Faculté de
Médecine, 27 Boulevard Jean Moulin, 13005 Marseille Cedex 05, France. Accepted for publication 21 October 1998
BACKGROUND Keywords:
Reg protein;
serum;
cystic fibrosis;
pancreatic
insufficiency
The
biological function of the Reg protein, a non-enzymic protein produced
in fairly large amounts by pancreatic acinar cells, remains elusive.
Its susceptibility to proteolysis leading to precipitation of the
proteolysis product at neutral pH suggests that it could contribute to
the protein plugging observed in cystic fibrosis (CF).
AIMSTo study its
behaviour in the serum of CF patients with or without pancreatic
insufficiency and to compare it with that of other pancreatic secretory proteins.
PATIENTS170 patients
(93 with CF, 55 controls, and 22 with chronic pancreatitis) were studied.
METHODSReg protein
was measured using a specific enzyme immunoassay and its molecular form
in CF sera was characterised by gel filtration. Molecular gene
expression was investigated by dot-blot hybridisation.
RESULTSReg protein
was present in all CF sera studied from patients with or without
pancreatic insufficiency, and in all cases the level was significantly
higher than in controls. Its chromatographic behaviour in CF sera was
identical with that of the protein present in normal serum. No
correlation was found between the levels of Reg protein and
trypsin(ogen) (or lipase) in CF, nor in control sera or normal
pancreatic juice. Molecular gene expression of the corresponding
proteins investigated in pancreatic tissues showed an absence of
correlation between the mRNA levels.
CONCLUSIONSReg
protein may not be a secretory exocrine protein like the digestive
enzymes but rather a hormone-like secretory substance with an endocrine
or paracrine function.
(Gut 1999;44:545-551)
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