Gut

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS REGISTER


[Advanced]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this link to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Add article to my folders
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Siavoshian, S
Right arrow Articles by Blottière, H M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Siavoshian, S
Right arrow Articles by Blottière, H M
Topic Collections
Right arrowRelated Article
Gut 2000;46:507-514 ( April )

Article

Butyrate and trichostatin A effects on the proliferation/differentiation of human intestinal epithelial cells: induction of cyclin D3 and p21 expression S Siavoshiana, J-P Segaina, M Kornprobsta, C Bonnetb, C Cherbutb, J-P Galmichea, H M Blottièrea b

a Centre de Recherche en Nutrition Humaine de Nantes, INSERM U539, CHU Hôtel-Dieu, Nantes, France, b INRA-LFDNH, Nantes, France

Correspondence to: Dr H M Blottière, Centre de Recherche en Nutrition Humaine, CRI-INSERM 95-08, CHU Hôtel-Dieu, place A Ricordeau, 44035 Nantes Cedex 01, France

Accepted for publication 20 October 1999

BACKGROUND---Sodium butyrate, a product of colonic bacterial fermentation, is able to inhibit cell proliferation and to stimulate cell differentiation of colonic epithelial cell lines. It has been proposed that these cellular effects could be linked to its ability to cause hyperacetylation of histone through the inhibition of histone deacetylase.
AIM---To analyse the molecular mechanisms of butyrate action on cell proliferation/differentiation and to compare them with those of trichostatin A, a well known inhibitor of histone deacetylase.
METHODS---HT-29 cells were grown in the absence or presence of butyrate or trichostatin A. Cell proliferation and cell cycle distribution were studied after DNA staining by crystal violet and propidium iodide respectively. Cell cycle regulatory proteins were studied by western blot and reverse transcription-polymerase chain reaction. Cell differentiation was followed by measuring brush border enzyme activities. Histone acetylation was studied by acid/urea/Triton acrylamide gel electrophoresis.
RESULTS---Butyrate blocked cells mainly in the G1 phase of the cell cycle, whereas trichostatin A was inhibitory in both G1 and G2 phases. Butyrate inhibited the mRNA expression of cyclin D1 without affecting its protein expression and stimulated the protein expression of cyclin D3 without affecting its mRNA expression. Trichostatin A showed similar effects on cyclin D1 and D3. Butyrate and trichostatin A stimulated p21 expression both at the mRNA and protein levels, whereas their effects on the expression of cyclin dependent kinases were slightly different. Moreover, butyrate strongly stimulated the activity of alkaline phosphatase and dipeptidyl peptidase IV, whereas trichostatin A had no effect. Finally, a six hour exposure to butyrate or trichostatin A induced histone H4 hyperacetylation. At 15 and 24 hours, histone H4 remained hyperacetylated in the presence of butyrate, whereas it returned to control levels in the presence of trichostatin A.
CONCLUSIONS---The data may explain how butyrate acts on cell proliferation/differentiation, and they show that trichostatin A does not reproduce every effect of butyrate, mainly because of its shorter half life.


Keywords: butyrate; cyclin D; p21; trichostatin A; colonic epithelial cells; histone acetylation

© 2000 by Gut

Related Article

The intracellular target of butyrate's actions: HDAC or HDON'T?
P R GIBSON
Gut 2000 46: 447-448. [Extract] [Full Text] [PDF]



This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
H. T. Tan, S. Tan, Q. Lin, T. K. Lim, C. L. Hew, and M. C. M. Chung
Quantitative and Temporal Proteome Analysis of Butyrate-treated Colorectal Cancer Cells
Mol. Cell. Proteomics, June 1, 2008; 7(6): 1174 - 1185.
[Abstract] [Full Text] [PDF]

Home page
 JPEN J Parenter Enteral NutrHome page
C. L. Kien, C. P. Peltier, S. Mandal, J. R. Davie, and R. Blauwiekel
Effects of the In Vivo Supply of Butyrate on Histone Acetylation of Cecum in Piglets
JPEN J Parenter Enteral Nutr, January 1, 2008; 32(1): 51 - 56.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
J. M. W. Wong and D. J. A. Jenkins
Carbohydrate Digestibility and Metabolic Effects
J. Nutr., November 1, 2007; 137(11): 2539S - 2546S.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
E. L. Karcher, M. M. Pickett, G. A. Varga, and S. S. Donkin
Effect of dietary carbohydrate and monensin on expression of gluconeogenic enzymes in liver of transition dairy cows
J Anim Sci, March 1, 2007; 85(3): 690 - 699.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
M. Bauer-Marinovic, S. Florian, K. Muller-Schmehl, H. Glatt, and G. Jacobasch
Dietary resistant starch type 3 prevents tumor induction by 1,2-dimethylhydrazine and alters proliferation, apoptosis and dedifferentiation in rat colon
Carcinogenesis, September 1, 2006; 27(9): 1849 - 1859.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Ramasamy, S. Singh, P. Taniere, M. J. S. Langman, and M. C. Eggo
Sulfide-detoxifying enzymes in the human colon are decreased in cancer and upregulated in differentiation
Am J Physiol Gastrointest Liver Physiol, August 1, 2006; 291(2): G288 - G296.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
M. R. Acharya, A. Sparreboom, J. Venitz, and W. D. Figg
Rational Development of Histone Deacetylase Inhibitors as Anticancer Agents: A Review
Mol. Pharmacol., October 1, 2005; 68(4): 917 - 932.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
N. DRUESNE, A. PAGNIEZ, C. MAYEUR, M. THOMAS, C. CHERBUY, P.-H. DUEE, P. MARTEL, and C. CHAUMONTET
Repetitive Treatments of Colon HT-29 Cells with Diallyl Disulfide Induce a Prolonged Hyperacetylation of Histone H3 K14
Ann. N.Y. Acad. Sci., December 1, 2004; 1030(1): 612 - 621.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
E. Gaudier, A. Jarry, H. M. Blottiere, P. de Coppet, M. P. Buisine, J. P. Aubert, C. Laboisse, C. Cherbut, and C. Hoebler
Butyrate specifically modulates MUC gene expression in intestinal epithelial goblet cells deprived of glucose
Am J Physiol Gastrointest Liver Physiol, December 1, 2004; 287(6): G1168 - G1174.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
N. Druesne, A. Pagniez, C. Mayeur, M. Thomas, C. Cherbuy, P.-H. Duee, P. Martel, and C. Chaumontet
Diallyl disulfide (DADS) increases histone acetylation and p21waf1/cip1 expression in human colon tumor cell lines
Carcinogenesis, July 1, 2004; 25(7): 1227 - 1236.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
Z. Y. Chen, S. Rex, and C.-C. Tseng
Kruppel-Like Factor 4 Is Transactivated by Butyrate in Colon Cancer Cells
J. Nutr., April 1, 2004; 134(4): 792 - 798.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
J. R. Davie
Inhibition of Histone Deacetylase Activity by Butyrate
J. Nutr., July 1, 2003; 133(7): 2485S - 2493.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Md. M. Rahman, A. Kukita, T. Kukita, T. Shobuike, T. Nakamura, and O. Kohashi
Two histone deacetylase inhibitors, trichostatin A and sodium butyrate, suppress differentiation into osteoclasts but not into macrophages
Blood, May 1, 2003; 101(9): 3451 - 3459.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Gelebart, T. Kovacs, J.-P. Brouland, R. van Gorp, J. Grossmann, N. Rivard, Y. Panis, V. Martin, R. Bredoux, J. Enouf, et al.
Expression of Endomembrane Calcium Pumps in Colon and Gastric Cancer Cells. INDUCTION OF SERCA3 EXPRESSION DURING DIFFERENTIATION
J. Biol. Chem., July 12, 2002; 277(29): 26310 - 26320.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
Q. Wang, N. Li, X. Wang, M. M. Kim, and B. M. Evers
Augmentation of Sodium Butyrate-induced Apoptosis by Phosphatidylinositol 3'-Kinase Inhibition in the KM20 Human Colon Cancer Cell Line
Clin. Cancer Res., June 1, 2002; 8(6): 1940 - 1947.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
C Domon-Dell, Q Wang, S Kim, M Kedinger, B M Evers, and J-N Freund
Stimulation of the intestinal Cdx2 homeobox gene by butyrate in colon cancer cells
Gut, April 1, 2002; 50(4): 525 - 529.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
P. Rosignoli, R. Fabiani, A. De Bartolomeo, F. Spinozzi, E. Agea, M.A. Pelli, and G. Morozzi
Protective activity of butyrate on hydrogen peroxide-induced DNA damage in isolated human colonocytes and HT29 tumour cells
Carcinogenesis, October 1, 2001; 22(10): 1675 - 1680.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. J. Wainwright, A. Lasorella, and A. Iavarone
Distinct mechanisms of cell cycle arrest control the decision between differentiation and senescence in human neuroblastoma cells
PNAS, July 31, 2001; 98(16): 9396 - 9400.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Bai and J. L. Merchant
Transcription Factor ZBP-89 Cooperates with Histone Acetyltransferase p300 during Butyrate Activation of p21waf1 Transcription in Human Cells
J. Biol. Chem., September 22, 2000; 275(39): 30725 - 30733.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. A. Cuff, D. W. Lambert, and S. P. Shirazi-Beechey
Substrate-induced regulation of the human colonic monocarboxylate transporter, MCT1
J. Physiol., March 1, 2002; 539(2): 361 - 371.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS REGISTER
Terms and conditions relating to subscriptions purchased online  ¦  Website terms and conditions  ¦  Privacy policy
Copyright © 2000 BMJ Publishing Group Ltd & British Society of Gastroenterology