Gut 2001;49:324-329 ( September )

Article

Gastric leptin and Helicobacter pylori infection T Azuma^a, H Suto^a, Y Ito^a, M Ohtani^a, M Dojo^a, M Kuriyama^a, T Kato^b

^a Second Department of Internal Medicine, Fukui Medical University, Fukui, Japan, ^b Faculty of Nursing and Welfare, Fukui Prefectural University, Fukui, Japan

Correspondence to: Dr T Azuma, Second Department of Internal Medicine, Faculty of Medicine, Fukui Medical University, Matsuoka-cho, Yoshida-gun, Fukui 910-1193, Japan. azuma{at}fmsrsa.fukui-med.ac.jp

Accepted for publication 12 February 2001

BACKGROUNDLeptin regulates feeding behaviour and therefore may be a mediator of anorexia associated with acute and chronic inflammation. Recently, leptin mRNA and leptin protein were found in the gastric epithelium.
AIMThe aim of the present study was to examine the effect of Helicobacter pylori infection on gastric leptin expression to investigate the pathophysiological role of gastric leptin.
METHODSSurgically resected human stomach tissues were subjected to immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR) to check for the presence of leptin in the human gastric epithelium. A total of 201 H pylori positive patients with chronic gastritis underwent eradication therapy for H pylori and were examined for the effect of infection cure in terms of body mass index (BMI) and serum leptin levels. Biopsy specimens from the gastric fundic mucosa were obtained from 40 of the 201 patients before and three months after eradication therapy. These samples were subjected to quantitative RT-PCR to examine the effect of eradication therapy on leptin expression in the gastric fundic mucosa.
RESULTSLeptin immunoreactive cells were detected in the lower half of the gastric fundic glands and a leptin PCR product was also found in the gastric fundic mucosa. H pylori infection significantly increased gastric leptin expression. In addition, cure of H pylori infection significantly reduced gastric leptin expression, with a concomitant increase in BMI. In contrast, serum leptin levels did not change significantly after cure of H pylori infection.
CONCLUSIONLeptin is present in the human gastric mucosa. Gastric leptin may play a role in weight gain after eradication of H pylori infection. Gastric leptin may have a local rather than systemic action.


Keywords: Helicobacter pylori; leptin; gastric mucosa; body mass index; gastritis

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© 2001 by Gut
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