Intestinal immunology

Genetically modified enterotoxigenic Escherichia coli vaccines induce mucosal immune responses without inflammation

Alexandra Daley¹, Roger Randall², Michael Darsley^2,*, Naheed Choudhry¹, Nicola Thomas², Ian R Sanderson¹, Nick M Croft¹, Paul Kelly¹

¹ Centre for Adult & Paediatric Gastroenterology, Institute of Cell and Molecular Science, Barts & The London, Queen Mary’s School of Medicine and Dentistry, London, UK
² Acambis Research Limited, Peterhouse Technology Park, Fulbourn Road, Cambridge, UK

Dr Paul Kelly , MD, FRCP, Centre for Adult & Paediatric Gastroenterology, Institute of Cell and Molecular Science, Barts & The London School of Medicine, Turner Street, London E1 2AD, UK; m.p.kelly{at}qmul.ac.uk

Objective: Enterotoxigenic Escherichia coli (ETEC) is a major cause of acute diarrhoea in children in the developing world, in travellers and in the military. Safe, effective vaccines could reduce morbidity and mortality. As immunity to ETEC is strain specific, the ability to create vaccines in vitro which express multiple antigens would be desirable. It was hypothesised that three genetically attenuated ETEC strains, one with a genetic addition, would be immunogenic and safe, and they were evaluated in the first licensed UK release of genetically modified oral vaccines.

Methods: Phase 1 studies of safety and immunogenicity were carried out at a Teaching Hospital in London. Varying oral doses of any of three oral vaccines, or placebo, were administered to volunteers of both sexes (n = 98). Peripheral blood responses were measured as serum antibodies (IgG or IgA) by ELISA, antibody-secreting cell (ASC) responses by enzyme-linked immunospot (ELISPOT), and antibody in lymphocyte supernatant (ALS) by ELISA. Mucosal antibody secretion was measured by ELISA for specific IgG and IgA in whole gut lavage fluids (WGLFs).

Results: Significant mucosal IgA responses were obtained to colonisation factors CFA/I, CS1, CS2 and CS3, both when naturally expressed and when genetically inserted. Dose–response relationships were most clearly evident in the mucosal IgA in WGLF. Vaccines were well tolerated and did not elicit interleukin (IL) 8 or IL6 secretion in WGLF.

Conclusions: Genetically modified ETEC vaccines are safe and induce significant mucosal IgA responses to important colonisation factors. Mucosal IgA responses were clearly seen in WGLF, which is useful for evaluating oral vaccines.

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