GASTRIC CANCER

Elevated plasma osteopontin associated with gastric cancer development, invasion and survival

Chun-Ying Wu^1,2,3, Ming-Shiang Wu⁴, En-Pei Chiang⁵, Cheng-Chung Wu^6,8, Yi-Ju Chen⁷, Chien-Jen Chen⁸, Nai-Hui Chi², Gran-Hum Chen², Jaw-Town Lin⁴

¹ Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan
² Division of Gastroenterology, Taichung Veterans General Hospital, Taichung, Taiwan
³ College of Public Health, China Medical University, Taichung, Taiwan
⁴ Division of Gastroenterology, Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan
⁵ Department Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan
⁶ Department of Surgery, Taichung Veterans General Hospital, Taichung, Taiwan
⁷ Department of Dermatology, Taichung Veterans General Hospital, Taichung, Taiwan
⁸ Institute of Epidemiology, College of Public Health, National Taiwan University, Taipei, Taiwan

Correspondence to:
Jaw-Town Lin
MD, PhD, Department of Internal Medicine, National Taiwan University Hospital, No. 7, Section 1, Chung-Shan S. Rd. Taipei, 10017, Taiwan; jawtown{at}ha.mc.ntu.edu.tw

Objective: Osteopontin (OPN) has been found to be valuable in diagnosis and predicting the prognosis of a variety of malignancies. The aims of the present study are to evaluate the usefulness of plasma OPN level for predicting gastric cancer development, invasion and survival.

Patients and Methods: One hundred and thirty two gastric cancer patients and 93 healthy controls were enrolled. Real-time quantitative reverse-transcription polymerase chain reaction and immunohistochemical staining were used to detect OPN expression in gastric cancer tissues. Plasma levels of OPN were measured by enzyme-linked immunosorbent assay. Plasma OPN levels were compared with gastric cancer development, clinicopathological features and outcomes.

Results: Expression of OPN mRNA was significantly higher in gastric cancer tissues compared with non-tumour tissues. Most OPN immunoactivity was localised to cancer cells. The median plasma OPN level was significantly higher in patients than in controls (p<0.0001), and significantly higher in patients with advanced stages, serosal invasion, lymph node metastasis, lymphatic invasion, venous invasion and liver metastasis. Logistic regression showed that high plasma OPN level (greater than 67.3 ng/ml) is significantly associated with advanced stages, serosal invasion, lymph node metastasis, lymphatic invasion, venous invasion and liver metastasis. Plasma OPN level demonstrated significant association with patient survival (p<0.0001), especially in the subgroups with invasive phenotypes. On Cox multivariate analysis, elevated plasma OPN level was an independent risk factor for poor survival (p<0.0001).

Conclusions: Elevated plasma OPN level is significantly associated with gastric cancer development, invasive phenotypes and survival. Plasma OPN level may have potential usefulness as a diagnostic and prognostic factor for gastric cancer.

Abbreviations: OPN, osteopontin

Keywords: osteopontin (OPN); plasma; gastric cancer; invasion; survival

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

Relevant Article

Digest

Robin Spiller and Emad El-Omar
Gut 2007 56: 741. [Extract] [Full Text] [PDF]

This article has been cited by other articles:

• Jain, A., McKnight, D. A., Fisher, L. W., Humphreys, E. B., Mangold, L. A., Partin, A. W., Fedarko, N. S. (2009). Small Integrin-Binding Proteins as Serum Markers for Prostate Cancer Detection. Clin. Cancer Res. 15: 5199-5207 [Abstract] [Full Text]  
• Snitcovsky, I., Leitao, G. M., Pasini, F. S., Brunialti, K. C. S., Mangone, F. R. R., Maistro, S., de Castro, G. Jr, Villar, R. C., Federico, M. H. H. (2009). Plasma Osteopontin Levels in Patients With Head and Neck Cancer Undergoing Chemoradiotherapy. Arch Otolaryngol Head Neck Surg 135: 807-811 [Abstract] [Full Text]  
• Hui, E. P., Sung, F. L., Yu, B. K.H., Wong, C. S.C., Ma, B. B.Y., Lin, X., Chan, A., Wong, W.-l., Chan, A. T.C. (2008). Plasma Osteopontin, Hypoxia, and Response to Radiotherapy in Nasopharyngeal Cancer. Clin. Cancer Res. 14: 7080-7087 [Abstract] [Full Text]  
• Mack, P. C., Redman, M. W., Chansky, K., Williamson, S. K., Farneth, N. C., Lara, P. N. Jr, Franklin, W. A., Le, Q.-T., Crowley, J. J., Gandara, D. R. (2008). Lower Osteopontin Plasma Levels Are Associated With Superior Outcomes in Advanced Non-Small-Cell Lung Cancer Patients Receiving Platinum-Based Chemotherapy: SWOG Study S0003. JCO 26: 4771-4776 [Abstract] [Full Text]