Intestinal inflammation

Mast cells and cellularity of the colonic mucosa correlated with fatigue and depression in irritable bowel syndrome

T Piche^1,2, M C Saint-Paul³, R Dainese¹, E Marine-Barjoan¹, A Iannelli⁴, M L Montoya¹, J F Peyron², D Czerucka², F Cherikh^1,5, J Filippi¹, A Tran¹, X Hébuterne¹

¹ Fédération d’Hépato-Gastroentérologie et de Nutrition Clinique, Hôpital de l’Archet, Pôle digestif, CHU de Nice, Nice France
² Unité INSERM 526, Nice, France
³ Laboratoire d’Anatomie Pathologique, Hôpital Pasteur, CHU de Nice, Nice, France
⁴ Service de Chirurgie digestive et de Transplantation hépatique, Hôpital de l’Archet, Pôle digestif, CHU de Nice, France
⁵ Psychiatry of liaison, CHU de Nice, Nice, France

Dr T Piche, Hôpital de l’Archet 2, Pôle digestif, Service de Gastroentérologie, BP 3079, 06202 Nice Cedex 3, France; tpiche{at}fc.horus-medical.fr

Background: A subset of patients with irritable bowel syndrome (IBS) have an increased number of mast cells (MCs) in the colonic mucosa. Psychological factors are believed to contribute to the course of IBS.

Aims: To examine associations between fatigue, depression and MCs of the colonic mucosa in IBS.

Methods: Colonic biopsies were taken from 50 Rome II IBS patients, 21 healthy controls and 11 depressed/fatigued patients without IBS. The cellularity of the lamina propria was determined as the number of inflammatory cells per high power field (hpf) through a 400x microscope. The Fatigue Impact Scale (FIS) and the short form Beck Depression Inventory (BDI) evaluated the severity of fatigue and depression.

Results: IBS patients had a significant increase in the cellularity of the lamina propria compared with controls or with depressed patients (mean (SD) 94.5 (48–110) vs 68 (58–82) and 78 (87–90) cells per hpf, p = 0.005 and p = 0.05, respectively), in particular of MCs (9.3 (5.6–11.7) vs 4.0 (2.7–6.8) and 4.3 (2.8–7.8) cells per hpf, p = 0.001 and p = 0.005, respectively). Both the FIS and BDI scores were significantly higher in IBS or in depressed patients than in controls (p<0.001). In IBS, the FIS score correlated significantly with the cellularity of the lamina propria (r = 0.51, p<0.0001) and MCs (r = 0.64, p<0.0001). In IBS, the BDI score correlated significantly with MCs (r = 0.29, p = 0.03).

Conclusions: Elevated MCs counts are a key feature of the low-grade inflammatory infiltrate in the caecal mucosa of IBS. Fatigue and depression are associated with mucosal cell counts, in particular MCs, suggesting that psychological factors are associated with the low-grade inflammatory infiltrate in IBS.

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