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Different DNA changes in primary and recurrent hepatocellular carcinoma.
  1. S F Ding,
  2. R P Jalleh,
  3. C B Wood,
  4. L Bowles,
  5. J D Delhanty,
  6. J Dooley,
  7. N A Habib
  1. University Department of Surgery, Royal Free Hospital School of Medicine, London.

    Abstract

    DNA restriction fragment length polymorphism analysis was carried out on a primary and recurrent hepatocellular carcinoma in a hepatitis B virus negative patient. For the primary tumour, allele losses were found on the short arm of chromosome 17 (probe: p144-D6, 17p13) and the long arm of chromosome 5 with the probe Lambda MS8 (5q35-qter); other probes showed either no allele loss or a non-informative pattern. The recurrent cancer also showed allele loss with p144-D6, but not with Lambda MS8. In addition, the recurrent tumour had allele losses with Lambda MS43 (12q24.3-qter), pYNZ22 (17p13), and DNA rearrangement revealed by the probe Lambda MS32 (1q42-43), a pattern not seen in the primary lesion. These results indicate that the second hepatocellular carcinoma was of independent clonality and probably represents a de novo neoplasm rather than a recurrence.

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