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Direct secretory effect of interleukin-1 via type I receptors in human colonic mucous epithelial cells (HT29-C1.16E).
  1. A Jarry,
  2. G Vallette,
  3. J E Branka,
  4. C Laboisse
  1. Groupe de Recherche 'Fonctions Sécrétoires des Epithéliums Digestifs', CJF INSERM 94-04, Nantes, France.

    Abstract

    The stable differentiated human colonic epithelial cell line, HT29-C1.16E, was used to study the effects of interleukin-1 (IL-1) on mucin exocytosis. The main findings include: (a) IL-1 stimulated a rapid release of mucin from filter grown HT29-C1.16E cells, this effect being dose related; (b) this secretory effect was abolished in the presence of the blocking monoclonal antibody M4 specific for IL-1 receptors type I, showing that IL-1 receptors type I mediated IL-1 action; (c) experiments based on chamber cultures showed that these receptors were located on the basolateral membranes of HT29-C1.16E cells; (d) finally, mRNA for IL-1 receptors type I were detected by reverse transcriptase-polymerase chain reaction in these cells. To extend these findings to the in vivo situation, the rapid stimulatory effect of IL-1 on mucin exocytosis may contribute to the wash out of noxious agents during mucosal inflammation.

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