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Immunology
T cells of the human intestinal lamina propria are high producers of interleukin-10
  1. J Braunsteina,c,
  2. L Qiaod,
  3. F Autschbachb,
  4. G Schürmannc,
  5. S Meuera
  1. aInstitute of Immunology, University of Heidelberg, Heidelberg, Germany, bInstitute of Pathology, University of Heidelberg, Heidelberg, Germany, cDepartment of Surgery, University of Heidelberg, Heidelberg, Germany, dDepartment of Microbiology and Immunology and Obstetrics and Gynecology, Loyola University Medical Center, Chicago, USA
  1. Dr J Braunstein, Institute of Immunology, University of Heidelberg, Im Neuenheimer Feld 305, D-69150 Heidelberg, Germany.

Abstract

Background and aim —Some of the recently observed functional features characteristic of immunocompetent cells residing in the human intestinal lamina propria could be mediated by interleukin- 10 (IL-10). To investigate the role of IL-10 in the human intestinal mucosa, the regulation of IL-10 production by lamina propria T lymphocytes (LPL-T) was determined and compared with that of peripheral blood T lymphocytes (PBL-T).

Methods —Following activation by using different stimuli, IL-10 release by LPL-T and PBL-T into the supernatant was measured by enzyme linked immunosorbent assay (ELISA). In parallel, cell growth was determined by [3H]-thymidine incorporation.

Results —Neither LPL-T nor PBL-T release IL-10 constitutively. Triggering through CD2 or the T cell receptor (TCR)/CD3 complex in the presence of autologous monocytes induces significantly greater IL-10 secretion by LPL-T than by PBL-T. Engagement of the CD45 receptor enhances IL-10 release and proliferation of CD2 triggered CD45RO+ PBL-T. In contrast, it reduces CD2 induced IL-10 production by LPL-T without altering cell growth significantly .

Conclusions—Activated LPL-T release relatively high amounts of IL-10. Enhanced IL-10 production by activated LPL-T, in comparison with activated PBL-T, is not only related to the presence of a higher proportion of CD45RO+ T cells in the intestinal lamina propria, but is also caused by increased sensitivity of LPL-T to CD2 co-stimulation. The differential responsiveness of LPL-T, compared with PBL-T, to CD45 engagement demonstrates that CD45 could be involved in the altered CD2 reactivity of LPL-T.

  • CD2
  • CD45
  • interleukin 10
  • lamina propria
  • T cell subsets
  • T lymphocytes

https://doi.org/10.1136/gut.41.2.215

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