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Cancer
Peutz-Jeghers polyps, dysplasia, and K-ras codon 12 mutations
  1. M M Entiusa,
  2. A M Westermanb,
  3. F M Giardielloe,
  4. M-L F van Velthuysenc,
  5. M M Polaka,
  6. R J C Slebosa,
  7. J H P Wilsonb,
  8. S R Hamiltond,
  9. G J A Offerhausa
  1. aDepartment of Pathology, Academic Medical Centre, University of Amsterdam, The Netherlands, bDepartment of Internal Medicine II, University Hospital Rotterdam, The Netherlands, cDepartment of Pathology, University Hospital Rotterdam, The Netherlands, dDepartment of Pathology and Oncology Centre, The Johns Hopkins University School of Medicine, Baltimore, USA, eDepartment of Medicine, Division of Gastroenterology, The Johns Hopkins University School of Medicine, Baltimore, USA
  1. Professor Dr G J A Offerhaus, Department of Pathology, Academic Medical Centre, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.

Abstract

Background—Peutz-Jeghers syndrome (PJS) is a rare, autosomal dominant, polyposis syndrome, associated with an increased risk of gastrointestinal and extragastrointestinal malignancy. Occasionally dysplasia occurs in PJS polyps.

Aims—In colorectal carcinomas, mutations in codon 12 of the K-ras oncogene are common and are found at similar frequency in precursor adenomas. Therefore, K-rascodon 12 point mutations in PJS polyps were evaluated.

Materials and methods—Fifty two PJS polyps, including four with dysplasia, collected from 19 patients with PJS, were analysed for mutations in the K-ras codon 12 by a mutant enriched polymerase chain reaction procedure, followed by allele specific oligodeoxynucleotide hybridisation.

Results—A K-ras codon 12 mutation was identified in one colonic polyp with dysplasia. The mutation was found in the non-neoplastic epithelial cells and not in the dysplastic component of the polyp.

Conclusions—K-ras codon 12 point mutations are very rare in PJS polyps, by contrast with colorectal adenomas. The findings support previous evidence that there seems to be no intrinsic relation between K-ras codon 12 mutation and dysplasia.

  • Peutz-Jeghers syndrome
  • polyps
  • dysplasia
  • K-ras codon 12 mutations

https://doi.org/10.1136/gut.41.3.320

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