Table 2
Western blot analysis of anti-colon antibody antigenic determinants using Caco-2 cell homogenate as antigen
| Caco-2 cell protein (kDa) | Ulcerative colitis (n=34) | Crohn’s disease (colon) (n=34) | Crohn’s disease (small bowel) (n=35) | Other bowel diseases (n=10) | Healthy controls (n=18) |
| 40.5 | 5 (15) | 7 (21) | 9 (26) | 0 | 2 (11) |
| 52 | 2 (6) | 10 (29) | 5 (14) | 1 (10) | 1 (6) |
| 60 | 3 (9) | 20 (59) | 24 (69) | 0 | 1 (6) |
| 71 | 4 (12) | 10 (29) | 4 (11) | 1 (10) | 1 (6) |
| 77 | 0 | 8 (24) | 0 | 0 | 1 (6) |
| 83 | 5 (15) | 10 (29) | 10 (29) | 0 | 2 (11) |
| 90 | 4 (12) | 13 (38) | 3 (9) | 0 | 3 (17) |
| 105 | 7 (21) | 1 (3) | 3 (9) | 0 | 0 |
| 113 | 8 (24) | 2 (6) | 0 | 0 | 1 (6) |
-
Values shown are the number that were positive with percentage in parentheses. Only the major protein bands detected by the sera are listed, the many bands bound by less than 20% of subjects being excluded.
