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  • Interleukin 1 and interleukin 1β converting enzyme (caspase 1) expression in the human colonic epithelial barrier. Caspase 1 downregulation in colon cancer

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Neoplasia
Interleukin 1 and interleukin 1β converting enzyme (caspase 1) expression in the human colonic epithelial barrier. Caspase 1 downregulation in colon cancer
  1. A Jarrya,
  2. G Vallettea,
  3. E Cassagnaub,
  4. A Moreaub,
  5. C Bou-Hannaa,
  6. P Lemarrea,
  7. E Letessierc,
  8. J-C Le Neelc,
  9. J-P Galmiched,
  10. C L Laboissea,b
  1. aCJF INSERM 94–04, Faculté de Médecine, CHU, Nantes, France, bService d’Anatomie Pathologique, CHU, Nantes, France, cClinique Chirurgicale A, CHU, Nantes, France, dService d’Hépato-Gastro-Entérologie, CHU, Nantes, France
  1. Dr C Laboisse, CJF INSERM 94–04, Faculté de Médecine, 1 Rue Gaston Veil, 44035 Nantes Cedex 01, France (email: laboisse{at}sante.univ-nantes.fr).

Abstract

BACKGROUND Interleukin (IL) 1β converting enzyme (now known as caspase 1) is able to process pro-IL-1β into its active form and is involved in proapoptotic signalling.

AIMS To characterise IL-1 and caspase 1 expression in human colonic epithelial cells.

METHODS Intracellular IL-1 content (IL-1α and IL-1β) was measured by ELISA in freshly isolated human normal colonocytes. Caspase 1 expression was determined both at the mRNA level using in situ hybridisation and reverse transcription polymerase chain reaction, and at the protein level by immunoblotting experiments using antibodies specific for the proform of caspase 1 and for its cleavage forms.

RESULTS Low amounts of IL-1β were found in nearly all preparations (92%), and IL-1α was detected in only 45% of human colonocyte preparations. The normal colonic epithelium strongly expressed caspase 1, both at the mRNA level and at the protein level in its latent form. In contrast, caspase 1 was not expressed in colon cancer (primary colonic adenocarcinomas and cancer cell lines).

CONCLUSIONS The demonstration that the human colonic epithelial barrier is able to express caspase 1 and its substrate IL-1β reinforces the concept that, under certain conditions, the epithelium could trigger an inflammatory reaction. In addition, the finding that caspase 1 was downregulated in colonic adenocarcinomas supports the concept that disrupted apoptosis pathways may be involved in tumour formation and/or may confer resistance to treatment.

  • caspase 1
  • colonic epithelial cells
  • colon cancer
  • interleukin 1
  • interleukin 1β converting enzyme

  • Abbreviations used in this paper

    IL
    interleukin
    ISH
    in situ hybridisation

    • https://doi.org/10.1136/gut.45.2.246

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    • Abbreviations used in this paper

      IL
      interleukin
      ISH
      in situ hybridisation
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