PCR HLA class II genotyping results
| Case | Method | HLA-DRB1 | HLA-DRB3, 4, 5 | HLA-DQB1 | Conclusion | |
|---|---|---|---|---|---|---|
| 1 | Dysplastic tissue | nSSP | 04, 13 | 53(4)1-151 | NA | Same HLA type |
| Reference tissue | SSP | 04, 13 | 53(4) | 06, 08 | ||
| 2 | Neoplastic tissue | nSSP | 0301, 15 | 51(5), 52(3) | NA | Same HLA type |
| Gastritis tissue | nSSP | 0301, 15 | 51(5), 52(3) | NA | ||
| Gastrectomy tissue | SSP | 0301, 15 | 51(5), 52(3) | 02, 06 | ||
| 3 | Neoplastic tissue | nSSP | 0301, 14 | 52(3) | NA | Contaminant |
| Reference tissue | nSSP | 0301, 04 | 52(3), 53(4) | NA | ||
| 4 | Neoplastic tissue | nSSP | 0301, 04 | 52(3), 53(4) | NA | Contaminant |
| Reference (pb) | SSP | 04, 04 | 53(4) | 07, 08 | ||
| Second patient | nSSP | 04, 10 | 53(4) | NA | ||
| Third patient | nSSP | 0701, 0701 | 53(4) | NA | ||
| Fourth patient (pb) | SSP | 12, 12 | No association | 0301/4, 0301/4 | ||
| Fifth patient (pb) | SSP | 0301, 13 | 52(3) | 02, 06 | ||
| Sixth patient (pb) | SSP | 0701, 1302 | 52(3), 53(4) | 02, 06 | ||
| Seventh patient (pb) | SSP | 0301, 1302 | 52(3) | 02, 06 | ||
| 5 | Dysplastic tissue | nSSP | 0103, 0301 | 52(3) | NA | Same HLA type |
| Reference tissue | nSSP | 0103, 0301 | 52(3) | NA | ||
| 6 | Malignant tissue | nSSP | 03, 11 | 52(3) | NA | Contaminant |
| Reference tissue | SSP | 01, 1402 | 52(3) | 05, 07 | ||
| 7 | Dysplastic tissue | nSSP | 01, 04 | 53(4) | NA | Contaminant |
| Reference tissue | SSP | 12, 1302 | 52(3) | 06, 07 | ||
| 8 | Dysplastic tissue | nSSP | 0301, 13 | 52(3) | NA | Contaminant |
| Reference tissue | SSP | 0301, 14 | 52(3) | 021-152 | ||
| 9 | Dysplastic tissue | nSSP | 01, 15 | 51(5) | NA | Contaminant |
| Reference tissue | SSP | 15, 15 | 51(5) | 06 | ||
| Second patient | SSP | 01, 15 | 51(5) | 07, 08 | ||
| 10 | Neoplastic tissue | nSSP | 0301, 11 | 52(3) | NA | Contaminant |
| Remaining tissue | nSSP | 03, 14 | 52(3) | NA | ||
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SSP, direct PCR-SSP genotyping; nSSP, nested PCR-SSP genotyping; pb, peripheral blood derived DNA (all other results derived from formalin fixed, paraffin wax embedded DNA).
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↵1-151 The expected DRB3 (DR52) gene was not detected.
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↵1-152 The expected DQB1*05 allele was not detected.
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In cases 1, 2, and 5, the chance of two unselected white Western individuals possessing the same HLA-DRB1 alleles is: case 1 (HLA-DRB1*04, 13) 6%; case 2 (HLA-DRB1*0301, 15) 8%; case 5 (HLA-DRB1*0103, 0301) <1%.4 11
