Table 1
Sequencing data
| Patient | Specimen | Date | Codon 528 | Codon 552 |
| Patient 1 | #1 | 09/09/94 | Leucine | Methionine |
| #2 | 11/07/95 | Methionine | Valine | |
| #3 | 08/12/95 | Methionine | Valine | |
| #4 | 18/03/96 | Methionine | Valine | |
| #5 | 16/05/96 | Methionine | Valine | |
| #6 | 24/07/96 | Methionine | Valine | |
| #7 | 04/09/96 | Leucine | Methionine | |
| Patient 2 | #8 | 04/08/95 | Leucine | Methionine |
| #9 | 19/09/96 | Methionine | Valine | |
| #10 | 18/03/97 | Methionine | Valine | |
| #11 | 19/05/97 | Leucine and methionine | Methionine, valine, and isoleucine1-150 | |
| Patient 3 | #12 | 20/03/96 | Leucine | Methionine |
| #13 | 28/05/97 | Leucine | Isoleucine | |
| Patient 4 | #14 | 19/12/94 | Leucine | Methionine |
| #15 | 17/10/95 | Leucine | Isoleucine | |
| #16 | 12/12/95 | Leucine | Isoleucine | |
| #17 | 26/06/96 | Leucine | Methionine | |
| #18 | 04/08/96 | Leucine | Methionine | |
| #19 | 04/06/97 | Leucine | Methionine |
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Part of the polymerase gene (nt 446–863) was amplified by PCR and directly sequenced using the same primers and an automatic sequencer. Multiple serum samples were examined (#1 to #19) and are referred to in the text and figures. Multiple changes were observed, and the amino acids at polymerase codon positions 528 and 552 are shown.
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↵1-150 Direct sequencing of serum #11 suggested that a mixture of viral species was present—leucine or methionine at position 528, and methionine and valine or isoleucine at 552.
