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Control of peripheral sympathetic prevertebral ganglion neurones by colonic mechanosensory afferents
  1. S M Miller
  1. Mayo Clinic, Department of Physiology and Biophysics, 8 Guggenheim Building, Mayo Foundation, 200 1st Street, SW Rochester, Minnesota 55905, USA
  1. Dr S M Miller. millers{at}mayo.edu

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Sympathetic innervation of the gastrointestinal tract arises from abdominal prevertebral ganglia (PVG) comprising the coeliac, superior mesenteric, and inferior mesenteric ganglia. These ganglia are integrating centres between the central nervous system and enteric nervous system and their output regulates intestinal motility, intestinal blood flow, and water and electrolyte secretion.1 Much of the synaptic input to PVG neurones arises from cholinergic mechanosensory neurones whose cell bodies lie in the gut wall and provide the PVG with information about the mechanical state of the gut. The mechanosensory (“intestinofugal”) neurones and their axons constitute the afferent limb of a peripheral reflex between enteric and sympathetic PVG neurones. The efferent limb of the reflex consists of the PVG neurones and their axons, which carry inhibitory sympathetic output back to the gut.2Experiments were performed in vitro to examine the peripheral reflex loop between colonic enteric neurones and sympathetic superior mesenteric ganglion (SMG) neurones in mouse. Preparations consisted of the SMG attached via intermesenteric and colonic nerves to a segment of distal colon.3 The distal end of the colonic segment was attached to a fluid filled reservoir. With this set up, the segment could empty its contents into the reservoir during a contraction and then refill during relaxation. Reflex activity was monitored intracellularly from SMG neurones while simultaneously recording intraluminal volume and muscle wall tension from the colonic segments.4 The results showed that colonic mechanosensory input to the SMG increased when the colon distended during filling, decreased and was absent when colonic contraction emptied the segment, and increased again when the colon relaxed and refilled (fig 1). These results suggested that the colonic mechanosensory afferent neurones monitored changes in intracolonic volume. To test this hypothesis, the colon segment was manipulated to prevent it from emptying during contraction or refilling after contraction. When the colon segment was prevented from emptying, the mechanosensory afferent input to the SMG neurones was not diminished during contraction. Conversely, if the colon segment could not refill after emptying, mechanosensory input to the SMG was absent until the moment when the colon was permitted to refill. To examine whether colonic mechanosensory afferents are activated by distension of the longitudinal, circular, or both muscle layers, the colon segment was made into a flat sheet and the sheet was stretched in the direction of the longitudinal or circular muscle axis while simultaneously recording electrical activity of SMG neurones.5 The results showed that the mechanosensory afferent pathway was activated when the colon was stretched in the circular muscle but not in the longitudinal muscle axis, suggesting that the mechanosensory endings in the colon wall are functionally arranged with the circular muscle layer. Retrograde labelling techniques were used to identify the cell bodies and processes of the colonic intestinofugal neurones.6 Their cell bodies were found in the myenteric ganglia, usually only one, but sometimes two labelled neurones per myenteric ganglion. Confocal microscopy will be used in future studies to locate their mechanosensory endings in the colon wall.

Figure 1

Changes in excitatory colonic mechanosensory synaptic input (lower trace) to a mouse superior mesenteric ganglion neurone during spontaneous change in intracolonic pressure and volume (upper trace)

Recent studies have identified two messenger molecules which may mediate afferent input to PVG and modulate sympathetic efferent output, and thus may be potential targets for therapy. The first is carbon monoxide (CO), a gaseous molecule suggested to be an important signalling molecule in the body.7 Gastrointestinal transit is markedly altered in mice lacking haeme oxygenase 2 (HO-2), the biosynthetic enzyme for CO biosynthesis in the nervous system, suggesting that CO may play a role in regulating the gut.8Immunohistochemistry using two different polyclonal antibodies raised against HO-2 was performed to examine the distribution of CO producing enzyme in the extrinsic and intrinsic nerves to the gastrointestinal tract. Both antibodies produced the same result, that HO-2 immunoreactivity was found in enteric neurones, interstitial cells of Cajal, and in sympathetic PVG neurones, suggesting that these cells may be important sources of CO regulating the gastrointestinal tract.9 Development of specific pharmacological inhibitors of HO-2 is necessary for continued progress in understanding how and where CO can modify gastrointestinal function.

A second important messenger molecule may be the hormone leptin, a protein produced by adipocytes, that acts on neurones in the hypothalamus to control food intake and energy balance.10Immunohistochemistry with an antibody raised against the leptin receptor showed that sympathetic PVG and enteric ganglia of the stomach, small intestine, and colon of normally fed mice and rats contained abundant leptin receptor-like immunoreactivity (LR-like IR). These results suggest that peripheral autonomic neurones, such as those in feeding centres of the brain, may be involved in the action of leptin on regulating food intake and metabolism. Confocal microscopy and three dimensional reconstruction of dye filled and immunolabelled PVG neurones were used to examine the distribution of leptin receptors in PVG neurones.11 The results showed that LR-like IR was located intracellularly and was absent from the neuronal plasma membrane (fig 2). Double immunolabelling of PVG neurones showed almost complete colocalisation of LR-like IR and Golgi body immunoreactivity. The intracellular location of leptin receptors in sympathetic neurones is puzzling as it is unclear how leptin (which is too large to cross the plasma membrane freely) might interact with its intracellular receptor. It is possible that translocation of the leptin receptor from the Golgi body to the plasma membrane might occur under conditions when leptin levels are abnormally high (for example, in obese and diabetic animals) or abnormally low (for example, in lean or starved animals). Studies are now being undertaken to examine the distribution of leptin receptors in PVG and enteric ganglia from obese and starved animals.

Figure 2

Three dimensional reconstructed images of mouse superior mesenteric ganglion (SMG) neurones injected with lucifer yellow (LY) fluorescent dye and then immunostained for leptin receptors (LR) Left: LY filled neurones (white), LR (green). Right: LY filled neurones were made transparent revealing the intracellular distribution of LRs (green) and nuclei (pink).

Acknowledgments

Supported by NIH grant DK17632 to J Szurszewski.

References

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Footnotes

  • Abbreviations used in this paper:
    PVG
    prevertebral ganglia
    SMG
    superior mesenteric ganglion
    CO
    carbon monoxide
    HO-2
    haeme oxygenase 2
    LR-like IR
    leptin receptor-like immunoreactivity

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