Article Text

PDF

Sympathetic input into the enteric nervous system
  1. O Lundgren
  1. Department of Physiology, Gothenburg University, Medicinaregatan 11, 400 33 Gothenburg, Sweden

    Statistics from Altmetric.com

    The basic concepts of the autonomic nervous system of organs and tissues were formulated around the turn of the century and summarised in a classical monograph by Langley (1921).1 The detailed distribution of the sympathetic nerves was, however, not elucidated until it became possible to stain sympathetic neurones specifically using the Falck-Hillarp technique. When this technique was applied to the gastrointestinal tract the picture seen was in some respects surprising. At the time it was generally believed that most organs and tissues had a dual innervation (sympathetic and parasympathetic) with opposite effects on function. It seemed, therefore, puzzling when the Falck-Hillarp technique revealed that innervation of a major part of the gastrointestinal wall, the smooth muscle layers, was very scarce. Instead it was found that most adrenergic fibres make contact with one of the two major plexuses in the alimentary canal. Adrenergic innervation is seen in the mucosa, in particular around the crypt epithelium.2 The functional consequences of this distribution are discussed below.

    This brief review of the interplay between the sympathetic and enteric nervous systems will discuss the control of blood vessels, epithelial transport, motility, and endocrine cells (the enterochromaffin cells in particular).

    Blood flow

    There is dense adrenergic innervation of both precapillary and postcapillary vessels in the digestive tract. Stimulating the sympathetic nerves to the gut elicits a blood flow response which is very characteristic. Immediately on electrically stimulating the splanchnic nerves, pronounced vasoconstriction is observed which, however, subsides within a few minutes to reach a steady state level of blood flow. It seems probable that flow during steady state represents the “physiological” response to nerve activation. Intestinal flow resistance is thus only moderately increased (2–3 times) even when stimulating the sympathetic vasoconstrictor nerves at high rates (8–16 Hz). When investigating blood flow distribution within the intestinal wall it has been demonstrated that villus blood flow is not under neural influence during the steady state part of vasoconstriction. The decrease in blood flow occurs in the crypts and muscle layers.3

    The sympathetic nerves not only control resistance vessels; stimulation frequency dependent constriction of the veins is observed, decreasing regional blood volume by 40% at most. Furthermore, the capillary filtration coefficient is markedly decreased, which indicates that the number of perfused capillaries is attenuated, presumably secondary to constriction of precapillary sphincters.

    The enteric nervous system (ENS) also controls intestinal blood flow. In vivo studies have shown that mechanical stimulation of the intestinal mucosa evokes marked vasodilatation. This response is apparently neurally mediated as it is abolished by nerve blocking agents (tetrodotoxin or lidocaine). The vasodilatation cannot be blocked by cholinergic receptor blockers, suggesting that there is no cholinergic synapse in the reflex. Furthermore, the response is not influenced by “chronic” denervation of the intestine, implying that vasodilatation is not mediated via an axon reflex of the “classical” type. However, 5-hydroxytryptamine (5-HT) tachyphylaxis or serotonin receptor blockers attenuate the vasodilatation caused by mucosal mechanical stimulation. Based on such observations it was suggested that mechanical stimulation causes release of 5-HT from enterochromaffin (EC) cells which in turn stimulate dendrites located just underneath the intestinal epithelium.4 This is illustrated schematically in fig lA. It should be emphasised that the detailed arrangement of this vasodilator reflex is not known. Some observations made by Surprenant5 suggest the possibility that an axon reflex may be involved.

    In contrast with sympathetic control of motility and epithelial transport, vasodilatation evoked by mechanical mucosal stimulation is not influenced by stimulating sympathetic vasoconstrictor fibres.4 The nervous arrangement of the local vasodilator mechanism is apparently organised in a different way from that of motility and fluid transport.

    Fluid and electrolyte transport

    The morphological observations of the adrenergic innervation briefly summarised in the introduction suggest two sites of action for the control of epithelial transport: a direct action on epithelial cells and an indirect action on enteric neurones, in particular those of the submucous plexus. Evidence for the latter mechanism has been obtained in electrophysiological studies which have shown that the non-cholinergic submucous neurones, presumably controlling epithelial functions, are under adrenergic influence via inhibitory postsynapatic potentials.6 This action evokes a decrease in electrolyte and fluid tissue to lumen transport, for example. Interestingly, cholinergic submucous neurones do not receive any such innervation.6

    It is well established that many secretory states of the intestinal tract, including cholera, are evoked via activation of the ENS. Figure lB illustrates the simplest model for the nervous secretory reflex of the ENS stimulated by cholera toxin that can be constructed from our current knowledge. There is experimental evidence for the view that cholera toxin and some bile salts, at least in part, activate the ENS via release of 5-HT from EC cells. In line with the electrophysiological observation described above it has been shown that splanchnic nerve stimulation inhibits fluid secretion caused by cholera toxin. This effect is more pronounced the higher the rate of fluid secretion and is probably explained by a higher activity in the ENS when toxin induced fluid secretion is large. Hence the sympathetic inhibitory influence is dependent on the level of activity in the secretory nervous reflexes of the ENS (fig 1B).7 There is also experimental evidence for a direct action of neurally released noradrenaline on crypt enterocytes although this effect is less important than the nervous effect on the ENS.

    Figure 1

    (A) Schematic illustration of the vasodilator reflex elicited by mechanical stimulation of the intestinal mucosa. The reflex is probably activated via release of 5-hydroxytryptamine (5-HT) from enterochromaffin cells functioning as mechanoreceptors. (B) Sympathetic nervous control of secretory nervous reflexes of the enteric nervous system. Sympathetic fibres (SF) exert their action at a synapse in the submucosal plexus and possibly also in the myenteric plexus. (C) Schematic illustration of the sympathetic control of gastrointestinal motility. In analogy with the sympathetic control of fluid and electrolyte transport, SF exert their control at a ganglionic site. In the figure, SF inhibits the motor activity induced by parasympathetic fibres (PF). SF may also inhibit intrinsic excitatory motor reflexes. Inhibitory action of a neurone is indicated by a filled circle at the end of the neurone.

    Sympathetic nervous control of epithelial fluid transport in the small intestine can be reflexly activated by deloading baroreceptors in the aorta and neck arteries and/or intrathoracic volume receptors. Thus sympathetic nerves are part of the homeostatic system controlling fluid and electrolyte content of the body.8

    Motility

    As noted in the introduction, the Falck-Hillarp technique showed that few adrenergic fibres reached the muscle layers of the gastrointestinal tract. This implies that sympathetic control of the smooth muscle layers is to a large extent not a direct but an indirect action, except for the direct effect of blood borne catecholamines released from the adrenal glands. There is experimental evidence for adrenergic inhibition of local excitatory motor reflexes and/or extrinsic excitatory parasympathetic nervous activity. Hence the magnitude of the sympathetic effect is dependent on ongoing activity in, for example, the vagal excitatory nerves to the smooth muscle layers in a manner similar to that described for sympathetic control of fluid and electrolyte transport (fig 1C).9

    It is apparent from figure 1 that sympathetic nervous control of epithelial transport and motility is organised in a similar way, namely control is exerted at a synaptic site. The sympathetic nerve may exert its action at a synapse in at least two ways. It may inhibit the release of acetylcholine, for example, from an intramural synapse, and/or noradrenaline released from sympathetic nerves may hyperpolarise the membrane potential of the postsynaptic neurone. There is experimental evidence for both mechanisms. ENS control of blood flow, lacking at least a cholinergic synapse, is not influenced by sympathetic neurones.

    Enterochromaffin cells

    The gastrointestinal tract is the largest endocrine organ in the body, containing a large number of peptides/amines located in endocrine cells dispersed among the transporting epithelial cells. It is well established that different chemicals in the intestinal lumen can evoke the release of specific peptides. Hence glucose causes release of gastric inhibitory peptide, and long chain fatty acids evoke release of cholecystokinin and neurotensin. Endocrine cells may also be sensitive to luminal physical variables. EC cells have been proposed as mechanoreceptors, releasing 5-HT on distortion of the villi.

    Based on studies performed mainly in EC cells, it seems probable that release of hormones from endocrine cells of the gastrointestinal tract are under control of the autonomic nervous system. Figure 2 summarises, in a schematic manner, the adrenergic and cholinergic control of release of 5-HT from EC cells. There are both α and β adrenergic receptors on EC cells. Release studies performed in vivo indicate that α adrenergic agonists inhibit release of 5-HT from cells whereas the cholinergic influence has the opposite effect.10 As suggested in figure 2, these effects may be mediated via changes in intracellular cyclic adenosine monophosphate and/or Ca2+concentrations.

    Figure 2

    Adrenergic and cholinergic control of the release of 5-hydroxytryptamine (5-HT) from enterochromaffin cells. cAMP, cyclic adenosine monophosphate.

    Autonomic nervous control of endocrine cells of the gastrointestinal tract may have interesting functional implications. It is well established that the sensitivity of the muscle spindles in skeletal muscle is controlled from the central nervous system (CNS) via efferent fibres influencing the so called intrafusal muscle cells. One can envisage similar CNS control of the sensitivity of endocrine cells of the gastrointestinal epithelium via the receptors illustrated in fig2. Activation of these receptors may change the sensitivity of endocrine cells to luminal stimuli by influencing membrane potential. Thus the sympathetic nervous system may indirectly influence the activity of ENS neurones via control of luminal sensory receptors. In the context of the present symposium, this may be particularly interesting for those endocrine cells which, via release of peptides/amines, activate afferent neurones located adjacent to their basolateral membrane.

    References

    View Abstract

    Footnotes

    • Corresponding author: Professor O Lundgren.ove.lundgren{at}fysiologi.gu.se

    • Abbreviations used in this paper:
      5-HT
      5-hydroxytryptamine
      EC
      enterochromaffin
      CNS
      central nervous system
      ENS
      enteric nervous system

    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.