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Targets of enteric motor neurones: smooth muscle cells
  1. R K Goyal
  1. ACOS Research and Development, VA Boston Health Care System, VA Medical Center, 1400 VFW Parkway, West Roxbury, Massachusetts 02132, USA
  1. Dr R K Goyal. raj_goyal{at}hms.harvard.edu

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The cellular targets of enteric motor neurones are gastrointestinal smooth muscle cells, interstitial cells of Cajal (ICC), motor neurones themselves, neuroendocrine cells, mucosal secretory and absorptive cells, secretory glands, blood vessels, and immune cells.1 The effects of motor neurones on smooth muscles, particularly the circular muscle cells and ICCs, are precise in timing whereas their effects on other targets may be somewhat diffuse and long lasting. The enteric motor neurones themselves are targets for intrinsic sensory neurones, enteric interneurones, motor neurones, axons of extrinsic primary afferents, vagal-sacral preganglionic neurones, sympathetic postganglionic neurones, and local and endocrine mediators.

The major physiological neurotransmitters of motor neurones are acetylcholine (ACh), adenosine triphosphate (ATP), tachykinins (substance P and neurokinin (NK) A), vasoactive intestinal peptide (VIP)-pituitary activating cyclic AMP peptide, and nitric oxide (NO).1 Release of neurotransmitter such as ACh is inhibited by presynaptic muscarinic M2 receptors, tachykinin NK2 and NK3 receptors, and by NO.

Each of the motor neurotransmitters has a large number of receptor subtypes coupled to a diverse array of intracellular signalling pathways, sometimes producing opposing actions. Some of the important issues related to motor neuromuscular transmissions are:

(1)
Differences between the actions of endogenous neurotransmitters and the exogenously administered transmitter candidate.
(2)
Regional differences in the actions of motor neurone activation on different tissue layers and different regions of the same tissue layer of the same organ. There are also obvious differences based on organ system and animal species.
(3)
In several systems there may be redundancy so that block of one pathway may be compensated for by another parallel pathway serving a similar function.
(4)
Finally, during events such as peristalsis, there is a precisely timed sequential activation of inhibitory and excitatory neurones.

The major excitatory motor pathway involves acetylcholine and tachykinins (substance P and NKA) that are frequently colocalised in motor nerve endings. Prejunctional autoreceptors for acetylcholine (M2 muscarinic) and TK (NK2 and NK3) act to inhibit and therefore limit acetylcholine release. On electrical field stimulation, the cholinergic component of the action starts earlier and lasts for a shorter period of time than the tachykinergic component. The TK responses become more prominent with greater intensities of electrical stimulation. Finally, there are marked regional differences in cholinergic and TK innervation.2 Another interesting regional difference shown by cholinergic neurotransmission is the observation that while M2 muscarinic receptor coupled to Gi mediates contraction of oesophageal circular muscle, M3 muscarinic receptor coupled to Gq/11 mediates contraction of the lower oesophageal sphincter smooth muscle.3

The main inhibitory neurotransmitters are ATP, NO, and VIP (fig1).4 5 The nerve endings that release ATP are not known and nitric oxide synthase (NOS) and VIP are frequently colocalised. Based on their characteristics, two types of inhibitory junction potentials (IJP) can be identified: fast and slow (fig 2). ATP is responsible for fast IJP and the associated mechanical relaxation is mediated via the P2y purinoreceptor and apamin sensitive small conductance calcium activated potassium channel.6 7There are marked regional variations in the distribution of the purinergic inhibitory pathway. NO (NO redox form) derived from neuronal NOS is responsible for the slow IJP.8 The nitrergic slow IJP and associated mechanical relaxation is mediated via the cyclic guanosine monophosphate pathway that acts by suppressing resting chloride conductance.9Peptide VIP acts to cause on demand synthesis and release of NO from NOS containing nerve endings. VIP may also act as a parallel neurotransmitter to cause pharmacomechanically coupled relaxation via the cyclic adenosine monophosphate signalling pathway.10-12

Figure 1

Inhibitory pathways mediating relaxation of smooth muscle. IJP, inhibitory junction potentials; ATP, adenosine triphosphate; ACh, acetylcholine; cGMP, cyclic guanosine monophosphate; NO, nitric oxide; NOS, nitric oxide synthase; AP, action potential; VIP, vasoactive intestinal peptide; -R, receptor.

Figure 2

Pharmacology of fast and slow inhibitory junction potentials (IJPs). L-NA, Nw-nitro L-arginine; NFA, niflumic acid; ODQ, 1 H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one.  

In producing peristaltic contractions, the inhibitory and excitatory nerves work together to produce a sequence of inhibition followed by excitation (fig 3).13 Activation of inhibitory purinergic nerves (where operative) produces fast inhibition followed by a more prolonged inhibition due to activation of nitrergic nerves. On cessation of activation of the inhibitory nerves, a rebound excitation ensues. Moreover, after activation of the inhibitory nerves is over, excitatory nerves are activated. Excitation due to ACh and TK is superimposed on the rebound contraction that results in a contraction wave with cholinergic, tachykinergic, and rebound components. The contribution of these three components may vary depending on the experimental conditions and region of the gut.

Figure 3

Sequence of inhibition and excitation which may explain peristalsis.

References

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Footnotes

  • Abbreviations used in this paper:
    ICC
    interstitial cells of Cajal
    IJP
    inhibitory junction potentials
    ATP
    adenosine triphosphate
    ACh
    acetylcholine
    TK
    tachykinins
    VIP
    vasoactive intestinal peptide
    NK
    neurokinin
    NO
    nitric oxide
    NOS
    nitric oxide synthase

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