Article Text

PDF

The putative role of inflammation in the irritable bowel syndrome
  1. S M COLLINS,
  2. T PICHE,
  3. P RAMPAL
  1. McMaster University, Hamilton, Ontario, Canada and
  2. Federation des Maladies de l'Appareil Digestif
  3. Centre Hospitalier Universitaire de Nice
  4. Faculte de Medecine, Universitaire de Nice, Nice, France
  1. Professor S Collins, Federation des Maladies de l'Appareil Digestif, Centre Hospitalier Universitaire de Nice, Hopital de L'Archet 2, 151 Route de Saint-Antoine de Ginestiere, BP 3079, 06202 Nice Cedex 3, France. scollins{at}mcmaster.ca

Statistics from Altmetric.com

The irritable bowel syndrome (IBS) may be defined as a painful chronic abdominal symptom complex which is usually associated with altered bowel habit, and for which there is no discernible underlying structural abnormality. It is generally accepted that symptoms are generated by abnormalities of gut function, including altered sensory perception, abnormal motility and, in some patients, abnormalities of epithelial function. Behavioural factors are important, particularly in the reporting of symptoms. What is lacking is an understanding of the pathogenetic mechanisms that alter gut function. IBS is a heterogeneous condition, not only in its clinical presentation and pathophysiology, but also in terms of its pathogenesis. While both central and peripheral factors have been implicated in the pathogenesis of IBS, this article will restrict itself to an evaluation of the evidence supporting inflammation as a basis for altered gut function in IBS.

In considering the role of inflammation in IBS, one is prompted to make a comparison with asthma. Like IBS, asthma was once considered a psychosomatic disorder, particularly in non-atopic children. For more than 50 years the treatment of asthma focused on the pharmacological correction of abnormal end organ physiology (that is, airways hyperresponsiveness), an approach that is similar to current therapeutic approaches to IBS, and which are aimed at modulating motor activity or sensory perception. The subsequent discovery of inflammatory cells in the normally sterile broncheoalveolar lavage led to the recognition of asthma as an inflammatory condition. There are two reasons why a similar shift in thinking in IBS will be difficult. Firstly, the gut is normally in a state of controlled inflammation and the challenge of identifying a subtle increase in inflammatory cell number or composition is not to be underestimated. Secondly, IBS is intuitively more heterogeneous than asthma, and inflammation is unlikely to be a factor in all cases. Nevertheless, there is emerging evidence for a role of inflammation in the pathogenesis of a least a subset of IBS patients.

From a clinical point of view, there are two scenarios that prompt consideration of a role for inflammation in the pathogenesis of IBS. The first is the development of IBS in patients following gastroenteritis (post-infective IBS (PI-IBS)). This occurs in 7–31% of patients with gastroenteritis from a variety of microbial agents1-3 including parasites.4 Some studies have shown a persistent increase in the cellularity of the lamina propria and mucosa or an increase in lymphocytes in the colon of these patients.5 6 Taken together, these findings suggest that the inflammatory response to infection, rather than the infective agent itself, is the important factor in induction of altered colonic physiology and generation of IBS symptoms.7 The second scenario is that of IBS-like symptoms that occur with a higher than expected frequency in patients in remission from inflammatory bowel disease (IBD), particularly ulcerative colitis.8 It is likely that the inflammatory process during acute exacerbation of IBD induces sensory-motor changes in the colon that have been shown to persist during remission9 and which may be a basis for generation of IBS-like symptoms.

The observations made in these patient groups are supported by studies in animal models of infection and inflammation. It is well recognised that an inflammatory response, which is largely restricted to the intestinal mucosa, may cause profound changes in the function of smooth muscle,10 enteric nerves,11 and interstitial cells of Cajal.12 Some of these changes also occur at remote non-inflamed sites,13 producing extensive disturbances in gut physiology. These studies also demonstrate that the changes in gut physiology result from specific components of the inflammatory response, including T lymphocytes10 and macrophages,11 rather than simply the presence of the infective agent. Of particular relevance to the above described clinical scenarios are demonstrations of persistence of altered physiology after recovery from infection and resolution of the mucosal inflammatory response.14-17 In studies of primary nematode infection in the mouse, successful expulsion of the parasite and resolution of the inflammatory response was accompanied by dysfunction of intestinal muscle and enteric nerves14 for 4–6 weeks. In addition, there was evidence of increased substance P levels and altered sensory perception following balloon distension of the colon 4–6 weeks post-infection.15 16 While T cells are required for the initiation of the changes in muscle contractility during the acute infection, the changes that persist post-infection are maintained by cyclooxygenase 2 derived prostaglandin E2production by resident cells, including smooth muscle.17Since the persistent changes in muscle function could be reversed by a short course of corticosteroid, administered after recovery from infection, they reflect a low grade inflammatory process in the muscularis externa.

The concept that IBD may predispose to IBS is supported by recent work in animals. Changes in colonic smooth muscle contraction have been observed in animals recovering from hapten induced colitis; muscle hypercontractility emerged at 21 days post-colitis and was initiated by T cells.18 Other studies using models of experimental colitis have shown an increase in serotonin containing enterochromaffin cells19 but it is not known whether these persist after resolution of the inflammatory response, as has been observed in patients with PI-IBS.6

Animal studies provide clear demonstrations of the abilities of immune or inflammatory cells to infiltrate and alter function in the deeper neuromuscular layers.10 11 The limited data from humans suggest that a similar process may occur in some IBS patients. These studies were performed on full thickness biopsies or surgical specimens, and are therefore restricted to patients with severe functional disturbances. Full thickness surgical specimens from the colon of patients with “spastic colitis” revealed mast cell infiltration of the myenteric plexus and muscularis externa.20 Recently, laparoscopic assisted biopsies from patients with severe IBS revealed a prominent lymphocytic infiltration of the myenteric plexus.21 The extent to which these findings apply to milder forms of IBS is not known.

In the majority of studies on IBS, histological examination has been restricted to the mucosa and lamina propria but have nevertheless demonstrated an inflammatory presence in some cases. Increases in mast cell number have been demonstrated in the colon or terminal ileum of IBS patients.22 23 Other studies have identified lymphocytes and other cell types6 24 or simply an increased cellularity of the lamina propria.25Interestingly, in several of these studies the changes were more marked in the ileum or right colon, and the rectum often tended to be normal. In a recent report, Barbara et al in Bologna identified a 30% increase in cellularity, including mast cells, in the colonic mucosa of IBS patients compared with controls, using quantitative microscopy. In addition they found, using electron microscopy, that there was a closer anatomical proximity between nerve trunks and lymphocytes or mast cells, suggesting neuroimmune interactions in the pathogenesis of IBS.26

There is limited insight into the presence of inflammatory mediators in IBS. A previous study on IBS patients with specific food intolerances suggested increased prostaglandin production on double blind food challenge.27 Studies on unselected IBS patients have demonstrated increased inducible nitric oxide synthase and nitrotyrosine expression which was associated with lymphocyte activation24 and increased expression of interleukin (IL)-1β mRNA in mucosal biopsies.28 While no mechanistic interpretation can be placed on these findings, they suggest an inflammatory presence in the IBS patients studied.

A recent preliminary report has suggested that some IBS patients may be genetically susceptible to inflammatory conditions. The study examined 140 unselected IBS patients for the frequency of alleles that encode the high production of the counter inflammatory cytokines IL-10 (−1082*G/A) and transforming growth factor β (TGF-β) (+915*G). They found that the frequency of the IL-10 high producer (−1082*G) allele and the TGF-β high producer allele were both significantly reduced in IBS patients compared with healthy controls.29As these cytokines are anti-inflammatory, low secretors may be less efficient in downregulating responses to inflammatory stimuli such as enteric infection. It is possible that such an abnormality could determine which patients develop IBS following gastroenteritis. This is supported by the demonstration of higher IL-1β mRNA expression in the colon of patients with PI-IBS compared with those who recovered fully from the infection.28 The notion that patients with PI-IBS are more prone to inflammatory conditions is indirectly supported by the observation that patients with a more protracted acute illness are at higher risk for the development of PI-IBS.3

Stress and behavioural factors have also been shown to influence the development of IBS after gastroenteritis. While behavioural factors are clearly important in symptom reporting in IBS, it is also possible that the development of PI-IBS reflects a convergence of behavioural and inflammatory components. For example, prior stress has been shown to enhance the response to an inflammatory stimulus in animals30 and to increase intestinal permeability.31 These factors, in the presence or absence of a genetically determined susceptibility, could lead to a protracted inflammatory response to enteric infection and induce PI-IBS (see fig1). The increased permeability of the intestine recently described in patients with PI-IBS6 might provide a mechanism for maintenance of the condition by exposing the gut to luminal antigen and activating the mucosal immune system, as reflected by demonstration of increased numbers of lymphocytes in colonic biopsies from these patients.6

Figure 1

Schematic representation of the relationship between inflammation and the irritable bowel syndrome (IBS). Citations represent evidence, from basic or clinical research, that support the proposed model. IBD, inflammatory bowel disease.

In conclusion, animal studies have shown that mild inflammatory stimuli can perturb the sensory-motor system of the gut and that under certain conditions these functional perturbations may persist after resolution of the inflammatory response. This process may underlie the development of IBS in patients recovering from acute gastroenteritis or a relapse of IBD. Genetic as well as behavioural factors may influence susceptibility to inflammatory signals and play a role in the development of IBS in these situations. The extent to which inflammation contributes to the pathogenesis of the remainder of the IBS population remains unclear but there are morphological data implicating immune activation in the myenteric plexus of patients with severe IBS. Future research should seek to identify markers of inflammatory based IBS, particularly in PI-IBS patients, and explore new therapeutic options aimed at suppressing the ongoing low grade inflammatory/immune response.

View Abstract

Footnotes

  • Leading articles express the views of the author and not those of the editor and editorial board.

  • Abbreviations used in this paper:
    IBS
    irritable bowel syndrome
    PI-IBS
    post-infective IBS
    IBD
    inflammatory bowel disease
    IL
    interleukin
    TGF-β
    transforming growth factor β

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

References