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Mutations in serine protease inhibitor Kazal type 1 are strongly associated with chronic pancreatitis
  1. J P H Drenth,
  2. R te Morsche,
  3. J B M J Jansen
  1. Department of Medicine, Division of Gastroenterology and Hepatology, University Medical Center St Radboud, Nijmegen, the Netherlands
  1. Correspondence to:
    Professor J B M J Jansen, Department of Medicine, Division of Gastroenterology and Hepatology, University Medical Center St Radboud, PO Box 9101, 6500 HB Nijmegen, the Netherlands;


Background: Although chronic pancreatitis is associated with risk factors such as alcoholism, hyperparathyroidism, and hypertriglyceridaemia, little is known of the actual aetiology of the disease. It is thought that inappropriate activation of trypsinogen causes pancreatitis, and indeed in cases of hereditary pancreatitis mutations of cationic trypsinogen (PRSS1) have been described. As serine protease inhibitor Kazal type 1 (SPINK1) is a potent natural inhibitor of pancreatic trypsin activity, we hypothesised that SPINK1 mutations would be more common than expected among an unselected cohort of adult chronic pancreatitis patients.

Aims: To detect the prevalence of SPINK1 mutations in a cohort of chronic pancreatitis patients.

Methods: DNA was isolated from a cohort of 115 adult patients with chronic pancreatitis of alcoholic (n=72), hereditary (n=10), idiopathic (n=24), and miscellaneous (n=9) origin. We performed mutational analysis for two PRSS1 mutations (R122H, N29I) and four specific SPINK1 gene mutations (M1T, L14P, N34S, P55S) and compared the results with a control group of 120 healthy Dutch subjects.

Results: In six of the 10 patients that fulfilled the criteria for hereditary pancreatitis, but in none of the control subjects, mutations in the PRSS1 gene were found. In 14 patients we detected a SPINK1 mutation. Eleven patients were heterozygous for the N34S mutation and sequencing confirmed the homozygous state of N34S in a brother and sister. Two patients carried the P55S mutation, one as a compound heterozygote with N34S. The M1T and L14P SPINK1 mutations were not found in our cohort. The N34S mutation was detected in only two of 120 controls, while the P55S, M1T, and L14P mutations were absent in the same group. Patients with the N34S allele had a later onset of disease than those with PRSS1 gene mutations but earlier onset compared with the mutation negative group.

Conclusion: Identification of SPINK1 mutations in 12.2% of patients with adult alcoholic and idiopathic chronic pancreatitis suggests an important role for SPINK1 as a predisposing factor in adult chronic pancreatitis.

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Chronic pancreatitis is a progressive inflammatory disorder ultimately leading to irreversible structural changes resulting in functional impairment of exocrine and/or endocrine physiology.1 It is relatively frequent and the prevalence has been estimated at 50–75 cases per 100 000 inhabitants. The mortality rate approaches 50% within 20 years after diagnosis.2 It is an important problem as most patients with chronic pancreatitis require frequent and intensive medical care because of relapsing attacks of incapacitating abdominal pain.3 In the Western world, alcohol abuse is generally considered as a substantial risk factor for the development of chronic pancreatitis. In addition, other aetiological factors such as heredity, cigarette smoking, anatomical variations, and various metabolic disorders have been identified. In up to 30% of patients, association with any of the aforementioned risk factors is lacking and the disease is classified as idiopathic.1

A major breakthrough in the understanding of the pathogenesis of chronic pancreatitis stemmed from results obtained in families with hereditary pancreatitis. Hereditary pancreatitis (Mendelian Inheritance of Men 167800, occurs as an autosomal dominant hereditary trait and positional cloning mapped the susceptibility gene for chronic pancreatitis on chromosome 7q.4,5 A further study identified the cationic trypsinogen gene (protease, serine 1; PRSS1) as a causative gene. Mutational analysis of this gene in affected individuals revealed a missense mutation resulting in an arginine to histidine substitution at codon 122 (R122H).6 Subsequent efforts in separate families identified a second missense mutation, N29I.7,8 It was speculated that these defects lead to a “gain of function” and result in increased trypsin activity leading to autodigestion and pancreatitis. Although these findings may explain to some extent the aetiopathogenesis of pancreatitis in these patients, two observations indicate that other mechanisms may also be important in this respect. Firstly, some families with hereditary pancreatitis do not carry mutations in the PRSS1 gene and secondly, PRSS1 gene mutations are not frequently found in individuals without a family history of chronic pancreatitis.

Pancreatic serine protease inhibitor Kazal type 1 (SPINK1, also called pancreatic secretory trypsin inhibitor, PSTI) is a 79 amino acid polypeptide (AF286028) that operates as a potent inhibitor of trypsin activity. In a recent study in children with idiopathic or familial pancreatitis, substitution of asparagine by serine at codon 34 of the SPINK1 gene (N34S) was detected in 18 of 96 patients.9 It is not known whether the association of SPINK1 mutations is limited to children with pancreatitis or whether this concept can be extended to other forms of (adult) chronic pancreatitis. We therefore set out to study whether mutations in SPINK1 are associated with chronic pancreatitis in a non-selected population of adult patients and investigated the presence of possible genotype-phenotype correlations.


Selection of patients

The study was approved by the hospital's ethics committee. We first designed a computerised database of patients with chronic pancreatitis. We reviewed the records of all patients who had been referred from 1980 to 2000 to the University Medical Center St Radboud because of symptoms of chronic pancreatitis. A diagnosis of chronic pancreatitis was based on the presence of a typical history (recurrent upper abdominal pain, radiating to the scapula tip, relieved by leaning forward or sitting upright and increased after eating), suggestive radiological findings, such as pancreatic calcifications or pseudocysts, and/or pathological findings (pancreatic ductal irregularities and dilatations) revealed by endoscopic retrograde pancreaticography or magnetic resonance imaging of the pancreas before and after stimulation with secretin. In total, 165 records were available for review. We collected data with special emphasis on the cause of chronic pancreatitis, onset of symptoms, early (splenic vein thrombosis, pancreatic pseudocysts) and long term complications (steatorrhoea, diabetes mellitus), surgical procedures, use of narcotics, and smoking behaviour. Because our department actively participates in a project on pain management by thoracoscopic splanchnic denervation we were able to divide the characteristics of pain into two types.3 Type A pain represents recurrent pain attacks while type B pain indicates unrelenting continuous pain.10 Patients who had an estimated daily intake of alcohol of more than 60 g (females) or 80 g (males) for more than two years were classified as chronic pancreatitis of alcoholic origin.11 Idiopathic chronic pancreatitis was diagnosed when precipitating factors such as alcohol abuse, gall stones, trauma, medication, infection, metabolic disorders, and positive family history were absent.12 Hereditary pancreatitis was defined when chronic pancreatitis was present in two or more family members. A letter was sent to these patients and their general practitioner explaining the goal of the study and a request to submit a blood sample. In total, 115 DNA samples were available for this study and all patients gave informed consent before enrollment.

DNA studies

We extracted genomic DNA from whole blood according to established protocols using the Puregene DNA isolation kit (Gentra Systems, Minneapolis, Minnesota, USA). In order to identify disease associated mutations we applied the following approach. We first screened the most prevalent mutations (N29I and R122H) in the PRSS1 gene. The N29I mutation was detected using an allele specific polymerase chain reaction (PCR).13 Patients were screened for the R122H mutation using a PCR-restriction fragment length polymorphism. Briefly, the 50 μl reaction mixture contained 200 ng of genomic DNA, 10 mM Tris HCl (pH 9.0), 50 mM KCl, 0.1% Triton, 2 mM MgCl2, 0.25 mM dNTPs, 100 ng of sense primer (5`-GGTCCTGGGTCTCATACCTT-3`), 100 ng of antisense primer (5`-GTAATGGGCACTCGAAATGT-3`) and 3.0 U Taq-DNA polymerase to generate a 555 bp fragment. Cycling conditions were an initial step at 94°C for five minutes, then 35 cycles at 94°C for 30 seconds, 60°C for 30 seconds, 72°C for 30 seconds, and a final elongation step at 72°C for five minutes. PCR products were incubated with 10 U BbrPI (Roche Diagnostics, Mannheim, Germany) for two hours at 37°C. The digested PCR samples were electrophoresed on a 3% RESponse PCR agarose (Biozym, Landgraaf, the Netherlands) generating two fragments of 327 bp and 228 bp for the wild-type and two additional fragments of 208 bp and 20 bp when the R122H mutation was present.

In mutation negative patients with a positive family history for the disease, we sequenced all five exons of the PRSS1 using the primers shown in table 1. Cycling conditions were an initial step at 94°C for five minutes, then 35 cycles at 94°C for 30 seconds, 62.5°C/57.5°C (PCR 1/PCR 2) for 30 seconds, 72°C for one minute, and a final elongation step at 72°C for five minutes. The PCR products were purified after electrophoresis on an agarose gel with the Qiaex II gel extraction kit (Qiagen, Hilden, Germany) With the purified products we performed sequencing with the Thermo Sequenase CyTM5 Dye Terminator Kit (Amersham Pharmacia Biotech, Buckinghamshire, UK) according to the manufacturer's manual and loaded them onto the ALFexpress DNA sequencer (Amersham Pharmacia Biotech).

Table 1

Sequences of oligonucleotide primers for the PRSS1 gene

Next we analysed four specific mutations in the SPINK1 gene: M1T, L14P, N34S, and P55S. We looked for these mutations in all chronic pancreatitis patients using an allele specific PCR. Briefly, a 50 μl reaction was prepared containing 200 ng genomic DNA, 10 mM Tris HCl (pH 9.0), 50 mM KCl, 0.1% Triton, 2 mM MgCl2, 0.25 mM dNTPs, 3.0 U Taq-DNA polymerase, and 100 ng of sense, antisense, and mutation primers (see table 2). Cycling conditions were an initial step at 94°C for five minutes and then 35 cycles at 94°C for 30 seconds, 59°C for 30 seconds, 72°C for 30 seconds, and a final elongation step at 72°C for five minutes. This allele specific PCR generated two fragments when the N34S mutation was present and one fragment when the wild-type was present. Hetero- or homozygosity of the N34S and P55S mutations were confirmed by complete sequencing of exon 3 of the SPINK1 gene using the sense primer as sequencing primer.

Table 2

Sequences of oligonucleotide primers for the SPINK1 gene

Population screening

To exclude the possibility that the observed mutations might be present in the population at large as a natural polymorphism, a population based study was performed. To this end 240 chromosomes of 120 unrelated healthy individuals of Dutch extraction were sequenced to detect the SPINK1 variants and the PRSS1 mutations R122H and N29I. Mean age of these healthy subjects was 35±11 years and 65 were female.

Statistical analysis

We used Fisher's exact test to estimate differences in prevalence among the three groups. The Wilcoxon signed ranks test was used to compare differences in age at onset. All p values were based on two sided comparisons. A p value of 0.05 was considered the lowest level of significance.


Characteristics of the patients

The clinical characteristics of the patients are shown in table 3. The cohort of 115 patients included 72 with alcohol related pancreatitis, 24 with idiopathic pancreatitis, 10 with a familial form of pancreatitis, and nine with various causes. There were 71 male and 44 female patients. Recurrent abdominal pain as a diagnostic problem or a therapeutic dilemma was the usual reason for referral.

Table 3

Clinical details of chronic pancreatitis patients and controls, and results of screening for mutations in the PRSS1 and SPINK1 genes

DNA studies

Table 3 indicates the results from mutational screening of the PRSS1 and SPINK1 genes. We detected six patients who carried a mutant PRSS1 gene. Two patients who had the N29I allele were siblings while a third patient was their niece. The R122H mutation was detected in a mother and her daughter and in a third unrelated patient from a family with hereditary pancreatitis. We then sequenced the whole coding region of the PRSS1 gene in four other familial cases of chronic pancreatitis but were unable to demonstrate mutations in any of the five coding exons of PRSS1 in these patients. We then tested all patients for four SPINK1 mutations using an allele specific PCR. In total, 14 chronic pancreatitis patients (12.2%) had at least one SPINK1 mutation, and none of the PRSS1 mutation positive patients carried a SPINK1 mutation. We detected the N34S allele in 13 patients (fig 1). In 11 of these patients the N34S mutation was heterozygous (fig 2A) and in the remaining two, brother and sister, it was present in the homozygous state (fig 2B). Sequencing of the 240 control alleles from 120 patients identified two N34S heterozygous mutations (0.83% of alleles, 1.67% of the healthy cohort) (p<0.0001) A heterozygous P55S allele was found in two chronic pancreatitis patients. One patient was a compound heterozygote and carried the P55S and N34S on different alleles. The P55S variant was present in none of the control chromosomes tested (p<0.0001). The M1T and L14P mutations were not detected in any of the patient or control samples.

Figure 1

Allele specific polymerase chain reaction analysis of the N34S mutation on exon 3 of the SPINK gene. The wild-type SPINK band is located at 285 bp. If the N34S mutation is present as in patient Nos 2, 6, and 8, a second band at 190 bp appears. The left lane shows size markers

Figure 2

(A) DNA sequence electrospherograms demonstrating a disease associated N34S SPINK1 mutation. Note the double peak at base 94 which results in a heterozygote A to G transition resulting in a substitution of asparagine by serine (N34S). (B) DNA sequence electrospherograms demonstrating a disease associated homozygote N34S SPINK1 mutation. Note that at base 93 there is a single G indicating that A is replaced by G on both alleles. This results in a homozygous substitution of asparagine by serine (N34S).

Assessment of genotype-phenotype correlation

Table 4 shows the cohort of chronic pancreatitis patients grouped according to the results of the DNA studies. Three groups were distinguished: those with a SPINK1 mutation (14 patients), those with a PRSS1 mutation (six patients), and those without any of the listed mutations (95 patients). The causes of pancreatitis did not differ among the mutation negative group and those with SPINK1 mutations. In five patients with a mutated SPINK1 allele, chronic pancreatitis was associated with alcohol abuse although one patient reported an alcohol intake at the time of diagnosis of only 25 g/day, lower than that mostly associated with alcoholic pancreatitis. The other nine patients with a mutated SPINK1 allele were diagnosed as idiopathic (five patients), familial (two patients), hyperparathyroidism (one patient), and annular pancreas (one patient). The group that was heterozygous or homozygous for SPINK1 mutations had an earlier onset of symptoms (26 (18) years v 38 (11) years; p<0.05) but a similar number of hospitalisations per patient compared with mutation negative patients (table 4). The two N34S homozygous patients had an earlier onset of the disease (11.5 and 18 years) but the course and severity of the disease were similar to heterozygous patients. Their parents, obligate N34S heterozygotes, did not suffer from symptoms suggestive of chronic pancreatitis. The mutation negative patients were similar to patients with a SPINK1 mutation with respect to abnormal findings on radiographic studies. Pancreatic pseudocysts were detected in 42 of 95 patients in the mutation negative group and in seven of 14 patients with a SPINK1 but in only one patient with PRSS1 mutations. Approximately 75% of patients from both groups underwent pancreatic surgery during the course of their disease, most commonly for pain management, and one third of patients from both groups were treated with thoracoscopic splanchnic nerve denervation. Compared with the mutation negative patients, diabetes mellitus as a sign of pancreatic endocrine failure was more common in patients with a SPINK1 mutation, although this did not reach statistical significance (table 4). Compared with the two other groups, patients with a mutant PRSS1 allele had a much earlier onset of symptoms (6 (5) years), required more hospitalisations (16 per patient), and consumed more narcotics (p<0.05). All patients with PRSS1 gene mutations had a positive family history for the disease compared with two patients in the mutation negative group and two in the SPINK1 group. One patient who was compound heterozygote for the N34S and P55S SPINK1 mutations had a very severe disease. This 54 year old male suffered from alcoholic pancreatitis for eight years. Pancreatitis was complicated by exocrine and endocrine insufficiency, diffuse pancreatic calcifications, and severe type B pain. Because of exacerbation of his chronic pain computer tomography of the pancreas was performed which disclosed a 3 cm tumour compatible with a pancreatic adenocarcinoma. He died eight months later.

Table 4

Distribution of various clinical parameters among patients with chronic pancreatitis groped according to the results of mutational screening for the SPINK1 and PRSS1 genes


Our investigation had two main objectives. Firstly, to assess whether SPINK1 mutations are associated with chronic pancreatitis and secondly, to determine whether patients who carry a SPINK1 mutation follow a different disease course compared with SPINK1 negative patients. We found a strong association between chronic pancreatitis and SPINK1 alleles in a cohort of 115 patients who were referred to us because of symptoms of chronic pancreatitis. In these patients the frequency of a SPINK1 mutation was seven times higher than that of a group of healthy controls. Most patients with a SPINK1 mutation had the N34S variation (13/14) but there were two patients with the P55S allele. Possession of these alleles has clinical consequences as carriers develop their disease earlier in life than patients without this mutation but later than patients with PRSS1 gene mutations. Although we detected minor differences in the clinical presentation between the groups that were mutation negative and SPINK1 positive, the phenotype of chronic pancreatitis was very similar when judged on the basis of the individual patient. Our study extends previous work because we included adult patients with chronic pancreatitis due to various causes.9 Indeed, our chronic pancreatitis cohort reflects the panel of patients seen in daily clinical practice as it has been estimated that in developed countries 60–70% of patients with chronic pancreatitis have a history of excessive alcohol intake while in 30% the disease is regarded as idiopathic.2 Our data showed that the SPINK1 allele was also associated with idiopathic pancreatitis, which is in line with a recent study by Chen et al who detected a prevalence of 6.4% of the N34S mutation among a group of 187 unrelated French subjects with idiopathic chronic pancreatitis.14 However, we also detected SPINK1 allele carriers in alcoholic chronic pancreatitis. In our cohort with chronic pancreatitis, 74 patients fulfilled the set criteria for alcoholic chronic pancreatitis and SPINK1 mutations were detected in five.11 Another study yielded similar results as the N34S mutation was seen in 16 of 274 patients with alcoholic chronic pancreatitis.15 The diagnosis of alcoholic pancreatitis is based on a clinical history of recurrent episodes of acute pancreatitis and a history of excessive alcohol intake. Despite these criteria, there is considerable confusion about the amount of alcohol intake that actually increases the risk of developing pancreatitis. Data vary from 25 g/day to more than 80 g/day for more than five years.10,16 It might be reasonable to postulate that SPINK1 mutations render patients more susceptible to risk factors such as alcohol, hypercalcaemia, and anatomical variations for the development of pancreatitis.9

Trypsinogen is regarded as the initiating molecule in the current model of the pathogenesis of pancreatitis. Mutations in PRSS1 are thought to result in either reduced degradation of the active trypsin or facilitated activation of trypsinogen. In this pathogenetic model SPINK1 acts as an inhibitor of activated trypsin and our findings confirm the important role of premature protease activity in the pathogenesis of pancreatitis.17 Indeed, incubation of equimolar quantities of trypsin and SPINK1 produces an inactive complex and it has been estimated that SPINK1 can inactivate 10–20% of active trypsin.17 Mutations in the SPINK1 gene may have functional consequences. Probably the mutations alter the protein structure so that it affects the efficient construction of an inactive complex.18

It is not clear whether SPINK1 gene mutations lead to a distinct form of pancreatitis with a separate mendelian inheritance pattern. The M1T mutation which destroys the translational initiation codon of SPINK1 has been observed in an affected grandfather and unaffected father which might implicate an autosomal dominant inheritance.9 The situation seems different in the N34S mutation as it was detected in one French family (without PRSS1 mutations) but did not segregate with the disease.19 If we regard SPINK1 mutations as an autosomal recessive hereditary trait one would expect that homozygote patients would develop very severe disease. This was not the case in the two patients from our cohort. They developed symptoms at an earlier age compared with heterozygotes but the disease course was similar. If SPINK1 leads to an autosomal recessive disease, heterozygotes would not develop chronic pancreatitis as was the case in our patients. Further, if modelled as autosomal dominant, heterozygotes would be at risk of pancreatitis. We found that the gene frequency was about 1% and given the estimate of 0.05–0.07% chronic pancreatitis in the Western population this would indicate an unlikely high percentage of non-penetrance.1 Witt et al analysed affected families (having the N34S mutation) and demonstrated a strong linkage disequilibrium suggesting that this gene was indeed responsible for the disease in these families9 but a recent study was unable to confirm this.18 These data may suggest that SPINK1 mutations do not act as a disease inducer per se but rather as a modifier gene. This reminds us of previous studies investigating mutations of the cystic fibrosis gene in patients with chronic pancreatitis. In one study, Cohn et al estimated that in patients with idiopathic pancreatitis, the frequency of a single cystic fibrosis transmembrane conductance regulator (CFTR) mutation was 11 times the expected frequency and the frequency of two mutant alleles was 80 times the expected frequency.20 In a cohort of 134 patients with chronic pancreatitis due to alcoholism, metabolic disease, or idiopathic disease, the frequency of CFTR mutations was nearly 2.5 times the expected frequency.21 This last study included 30 patients with idiopathic pancreatitis, and CFTR mutations were detected in 30% of patients.22 It might be of interest to study whether mutations in the SPINK1 gene are associated with CFTR mutations in patients with chronic pancreatitis. This would explain in part the low penetrance of the N34S mutation. Similarly, although PRSS1 positive patients from our cohort did not have SPINK1 mutations, it would be tempting to speculate that such composition would lead to a severe phenotype as both gene alterations increase trypsin activity in different ways.

This study possibly underestimates the prevalence of SPINK1 mutations in chronic pancreatitis patients as we only examined four specific SPINK1 mutations. Another study investigating familial pancreatitis identified additional mutations in exon 3 of the SPINK1 coding region. This D50E was present in 1/112 affected individuals. Another mutation, G77G, located in exon 4, was also present in a single case. One intron mutation (IVS3–2T>C) was described in three patients from one study.18 Given the very low frequency of these mutations, it is unlikely that testing these mutations in our cohort would have significantly altered the results presented here.

In conclusion, our study identified mutations of the SPINK1 gene as a risk factor for chronic pancreatitis. Furthermore, it has increased our understanding of the pathogenesis of pancreatitis, and it may increase our understanding of the clinical behaviour of the disease.


We thank Dr H van Goor, Department of Surgery, University Medical Center St Radboud, Nijmegen, the Netherlands, for making his database of chronic pancreatitis patients who underwent thoracoscopic denervation of the splanchnic nerve available to us. We also thank Dr FM Nagengast and Dr AHJ Naber, University Medical Center St Radboud, Nijmegen, the Netherlands, for referral of patients to us. We thank the patients and their general practitioners for their cooperation who made it possible to execute this study. We are indebted to Tevfik Gunenc, Faculty of Medicine, Catholic University Nijmegen, the Netherlands who entered the clinical data in the database. This study was supported by the “Netherlands Digestive Diseases Foundation” (WS 00-21). Dr Joost PH Drenth is an Investigator of the Royal Netherlands Academy of Arts and Sciences

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