Table 1
Primer pairs used to quantify iron related transcripts expressed in the duodenum
| Gene | Sense primer | Antisense primer | GenBank accession No (position) |
|---|---|---|---|
| Dcytb, duodenal cytochrome b; DMT1, divalent metal transporter 1; SFT, stimulator of Fe transport; FPN1, ferroportin 1; TfR1, transferrin receptor 1. | |||
| Dcytb | 5′-GCAGCGGGCTCGAGTTTA-3′ | 5′-TTCCAGGTCCATGGCAGTCT-3′ | AF354666 (137–235) |
| DMT1 | 5′-GGCTTTCTTATGAGCATTGCCTA-3′ | 5′-GGAGCACCCAGAGCAGCTTA-3′ | L33415 (289–385) |
| SFT | 5′-CTGTGCTCATTGAAGAGGACCTT-3′ | 5′-TCTGGTTGCTTTCTCAGTCACG-3′ | AA178012 (232–329) |
| FPN1 | 5′-TTGCAGGAGTCATTGCTGCTA-3′ | 5′-TGGAGTTCTGCACACCATTGAT-3′ | AF226613 (1670–1789) |
| Hephaestin | 5′-TTGTCTCATGAAGAACATTACAGCAC-3′ | 5′-CATATGGCAATCAAAGCAGAAGA-3′ | AF082567 (3803–3963) |
| HFE | 5′-CTGAAAGGGTGGGACTACATGTTC-3′ | 5′-GGACACCACTCCCAACTTCGT-3′ | U66849 (362–454) |
| TfR1 | 5′-TCATGAGGGAAATCAATGATCGTA-3′ | 5′-GCCCCAGAAGATATGTCGGAA-3′ | X57349 (2018–2118) |
| β-Actin | 5′-GACGGCCAAGTCATCACTATTG-3′ | 5′-CCACAGGATTCCATACCCAAGA-3′ | M12481 (652–740) |
