Article Text
Abstract
Background: Gastric mucosa associated lymphoid tissue (MALT) lymphomas are clinically subdivided into Helicobacter pylori dependent and independent, according to H pylori infection and the therapeutic course. In previous reports it has been suggested that H pylori independent cases develop from H pylori dependent cases, and sometimes transform into high grade diffuse large B cell lymphomas (DLBCLs).
Methods: To better understand the pathogenesis of H pylori dependent and independent MALT lymphomas, we analysed the methylation profiles of eight independent CpG islands, including p15, p16, p73, hMLH1, death associated protein kinase, MINT1, MINT2, and MINT31 in H pylori dependent and independent MALT lymphomas, DLBCLs, and H pylori associated chronic gastritis.
Results: We first confirmed that H pylori independent cases had a high incidence of t(11;18)(q21;q21) (4/8 cases) and aberrant BCL10 expression (7/8 cases) compared with H pylori dependent cases and gastric DLBCLs. In the methylation pattern study, all 13 H pylori dependent MALT lymphomas had more than four methylated loci while H pylori independent cases had less than two. According to the previous criterion, all H pylori dependent MALT lymphomas (13/13, 100%) and five of 10 (50%) DLBCLs were classified as CpG island methylator phenotype positive (CIMP+). In contrast, all H pylori independent MALT lymphomas were CIMP−.
Conclusion: The distinct methylation pattern together with lack of chromosomal translocation in H pylori dependent MALT lymphomas suggest that H pylori dependent and independent MALT lymphomas have a different pathogenesis.
- mucosa associated lymphoid tissue
- Helicobacter pylori
- methylation
- lymphoma
- MALT, mucosa associated lymphoid tissue
- DLBCLs, diffuse large B cell lymphomas
- CIMP, CpG island methylator phenotype
- API2, apoptosis inhibitor 2
- PCR, polymerase chain reaction
- RT, reverse transcriptase
- GAPDH, glyceraldehydes-3-phosphate dehydrogenase
- MSP, methylation specific PCR
- ROS, reactive oxygen species
- DAP, death associated protein
- MINT, methylated in tumours
- hMLH, human Mut L homologue
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- MALT, mucosa associated lymphoid tissue
- DLBCLs, diffuse large B cell lymphomas
- CIMP, CpG island methylator phenotype
- API2, apoptosis inhibitor 2
- PCR, polymerase chain reaction
- RT, reverse transcriptase
- GAPDH, glyceraldehydes-3-phosphate dehydrogenase
- MSP, methylation specific PCR
- ROS, reactive oxygen species
- DAP, death associated protein
- MINT, methylated in tumours
- hMLH, human Mut L homologue