Article Text


Lysine kinetics in preterm infants: the importance of enteral feeding
  1. S R D van der Schoor1,
  2. P J Reeds,
  3. F Stellaard2,
  4. J D L Wattimena1,
  5. P J J Sauer2,
  6. H A Büller1,
  7. J B van Goudoever3
  1. 1Erasmus MC-Sophia Children’s Hospital, Department of Paediatrics, Dr Molewaterplein 60, 3015 GJ, Rotterdam, the Netherlands
  2. 2University Hospital Groningen, Department of Paediatrics, Hanzeplein 1, 9713 GZ Groningen, the Netherlands
  3. 3Erasmus MC-Sophia Children’s Hospital, Department of Paediatrics, Dr Molewaterplein 60, 3015 GJ, Rotterdam, the Netherlands, and USDA/ARS Children’s Nutrition Research Centre, Department of Paediatrics, Baylor College of Medicine, 1100 Bates St, Houston, TX, 77030, USA
  1. Correspondence to:
    Dr J B van Goudoever
    Erasmus MC-Sophia Children’s Hospital, Department of Paediatrics, Division of Neonatology, Dr Molewaterplein 60, 3015 GJ, Rotterdam, the Netherlands;


Introduction: Lysine is the first limiting essential amino acid in the diet of newborns. First pass metabolism by the intestine of dietary lysine has a direct effect on systemic availability. We investigated whether first pass lysine metabolism in the intestine is high in preterm infants, particularly at a low enteral intake.

Patients and methods: Six preterm infants (birth weight 0.9 (0.1) kg) were studied during two different periods: period A (n = 6): 40% of intake administered enterally, 60% parenterally; lysine intake 92 (6) μmol/(kg×h); and period B (n = 4): 100% enteral feeding; lysine intake 100 (3) μmol/(kg×h). Dual stable isotope tracer techniques were used to assess splanchnic and whole body lysine kinetics.

Results: Fractional first pass lysine uptake by the intestine was significantly higher during partial enteral feeding (period A 32 (10)% v period B 18 (7)%; p<0.05). Absolute uptake was not significantly different. Whole body lysine oxidation was significantly decreased during full enteral feeding (period A 44 (9) v period B 17 (3) μmol/(kg×h); p<0.05) so that whole body lysine balance was significantly higher during full enteral feeding (period A 52 (25) v period B 83 (3) μmol/(kg×h); p<0.05).

Conclusions: Fractional first pass lysine uptake was much higher during partial enteral feeding. Preterm infants receiving full enteral feeding have lower whole body lysine oxidation, resulting in a higher net lysine balance, compared with preterm infants receiving partial enteral feeding. Hence parenterally administered lysine is not as effective as dietary lysine in promoting protein deposition in preterm infants.

  • enteral nutrition
  • lysine metabolism
  • parenteral nutrition
  • LRP, lysine release from protein breakdown
  • NOLD, non-oxidative lysine disposal
  • PDB, Pee Dee Belemnite

Statistics from

The primed constant intravenous infusion of amino acids labelled with stable isotopes has become the reference method for studying whole body protein dynamics.1–3 By measuring oxidation rates of specific amino acids directly, conclusions can be drawn on the excessive supply of a particular amino acid. Because of limitations in studying nutrient metabolism in preterm neonates, several studies in neonatal animals have been performed to quantify protein metabolism in vivo. These studies have shown that the portal drained viscera (intestines, pancreas, spleen, and stomach) utilise more than 50% of dietary protein intake for protein synthesis and biosynthetic pathways,4–7 and is a major site of lysine oxidation.8 Of particular importance is the utilisation rate by the intestine of essential amino acids. A high utilisation rate of essential amino acids by the intestine results in lower systemic availability of essential amino acids. We were specifically interested in lysine, which is the first limiting amino acid in milk fed mammalian newborns.9 Thus if the amount of protein in the diet is not adequate, the rate of protein synthesis and growth will be determined by the amount of lysine.

The neonatal piglet is generally considered to be a good model for the human infant with respect to rate and pattern of gastrointestinal tract development.10 In addition, amino acid patterns are similar between piglets and infants in terms of both plasma concentrations and requirements.11,12 However, there are no data on lysine kinetics in infants while in adults splanchnic lysine uptake has been found to vary between 5% and 35% of lysine intake.7,13

Largely as a result of their immature gastrointestinal tract, all preterm infants face a period of compromised enteral intake in their first weeks of life, and they frequently receive a combined parenteral and enteral nutrient supply. Based on our finding that absolute lysine utilisation by the intestine of neonatal pigs appears to be independent of lysine intake,8 we postulated that fractional first pass lysine utilisation would be much higher during a restricted enteral intake. Importantly, this would result in a lower systemic availability of dietary lysine. Therefore, we measured first pass lysine uptake in response to two different enteral intakes in neonates.

Apart from first pass lysine utilisation, the magnitude of intestinal lysine catabolism is also important to the nutrition of preterm newborns. The energy needed to sustain the high rate of protein turnover in the gut is largely derived from oxidation of non-essential amino acids.4,14 However, some essential amino acids, including lysine, are also oxidised, which leads to irreversible loss.8,15,16 Despite the general belief that the intestinal mucosa does not possess lysine catabolic enzymes,17,18 we showed that intestinal oxidation of dietary lysine in pigs accounted for approximately 30% of whole body lysine oxidation.6 Whether dietary lysine is oxidised by splanchnic tissues in humans has not yet been investigated. Quantification of the response of splanchnic and whole body lysine oxidation on the level of enteral protein intake in preterm infants was the second objective of this study.



The study protocol was approved by the Erasmus University Institutional Review Board. Written informed consent was obtained from parents. Splanchnic and whole body lysine kinetics were quantified in preterm infants during partial enteral feeding (period A) and full enteral feeding (period B). Patients eligible for this study were premature infants with a birth weight ranging from 750 g to 1250 g who were appropriate for gestational age according to the charts of Usher and McLean.19 Excluded from the study were infants who had congenital anomalies, or gastrointestinal or liver diseases. CRIB scores20 on the first day of life were all below 5. The clinical characteristics of the neonates are shown in table 1. They received a nutrient regimen according to our feeding protocol: a combination of breast feeding or formula (Nenatal; Nutricia, Zoetermeer, the Netherlands) and parenteral nutrition containing glucose, amino acids (Primene 10%; Clintec Benelux NV, Brussels, Belgium), and lipids (Intralipid 20%; Fresensius Kabi, Den Bosch, the Netherlands). Nenatal was given as sole enteral nutrition 12 hours before the start of the study and during the study days.

Table 1

Subject characteristics


The study design consisted of two periods of two consecutive study days (period A—study days 1 and 2, and period B—study days 3 and 4) (fig 1). During period A, infants received 40% enteral and 60% parenteral feeding, and during period B they received full enteral feeding. During period A, infants were implanted with both an arterial and intravenous catheter for infusion of tracers and withdrawal of blood samples. During period B, a peripheral intravenous catheter was available for infusion of tracers; blood samples were collected by heelstick. Withdrawing blood via a heelstick is obtaining blood from arterial capillaries while the heel is heated before collection to ensure easy collection. We assume that similar to the heated hand box technique, there is no difference in plasma enrichment.21

Figure 1

Schematic overview of study days 1 and 2 during period A, and study days 3 and 4 during period B.

In order to collect breath samples from these infants, we used the method described by Perman et al who used a nasal tube.22 This method has been used in children22 and preterm infants for collection of expiratory CO2 recovery after administration of 13C labelled substrates.23,24 We have validated this technique for the use of oxidation studies in preterm infants (Van der Schoor, unpublished data). Briefly, a 6 Fr gastric tube (6 Ch Argyle; Sherwood Medical, Tullamore, Ireland) was placed 1–1.5 cm into the nasopharynx, and a sample of 15 ml end tidal breath was taken slowly with a syringe. Duplicate aliquots of expired air from each sampling point were stored in vacutainers for analysis.

Three different stable isotope infusions were performed during each study day. Firstly, a primed two hour infusion (10.02 μmol/kg and 10.02 μmol/(kg×h)) of [13C]bicarbonate (99.0 mol% 13C; Cambridge Isotopes, Woburn, Massachusetts, USA) dissolved in sterile saline was administered at a constant rate. The 13C labelled bicarbonate infusion was immediately followed by two primed five hour infusions (9.77 μmol/kg and 9.77 μmol/(kg×h)) of [U-13C]lysine (97.0 mol% 13C; Cambridge Isotopes) and (9.31 μmol/kg and 9.31 μmol/(kg×h)) of [2H4]lysine (98.0 mol% 2H; Cambridge Isotopes). During both periods A and B, we administered the tracers in the following order: on study days 1 and 3, [U-13C]lysine was given via an intravenous catheter and [2H4]lysine via the intragastric catheter; on study days 2 and 4, the intravenous and intragastric routes were switched. All isotopes were tested and found to be sterile and pyrogen free before use in our studies. At time 0, baseline blood and breath samples were collected. During the last hour of each tracer infusion, breath samples were collected at 15 minute intervals and blood samples were obtained at 390 and 420 minutes. The total amount of blood withdrawn during a study day was 1.5 ml, which is less than 2% of blood volume in a 1000 g infant. Blood was centrifuged immediately and stored at −70°C for further analysis.

Analytical methods

Small aliquots of plasma (100 μl) were taken for measurement of plasma lysine concentrations by an Amino Acid Analyser (Biochrom Ltd, Cambridge, UK). Enrichment of [U-13C]lysine and [2H4]lysine in plasma was determined by gas chromatography-mass spectrometry as the N(O,S)-methoxycarbonylmethylester derivative according to Husek,25 with minor modifications. Breath samples were analysed for enrichment of 13CO2 on an isotope ratio mass spectrometer (ABCA; Europa Scientific, Van Loenen Instruments, Leiden, the Netherlands).26


The rate of lysine turnover was calculated by measuring tracer dilution at steady state as modified for stable isotope tracers, as previously described.27,28 The equations used to obtain the results are detailed in the appendix.


Data are expressed as mean (SD) values obtained from samples taken over the last hour of each tracer infusion. Statistical comparisons were performed using the Student’s t test. A value of p<0.05 was taken as statistically significant.


Six patients were studied during period A and four during period B (table 1). Body weight at the time of period A was 1.0 (0.1) kg and 1.3 (0.2) kg during period B. All infants were clinically stable with no clinical signs of septicaemia. Routine blood chemistry and haematology were all within normal limits, and there were no significant changes in these parameters 24 hours before and after the study. During period A, five of six infants received supplemental oxygen by a nasal prong, while during period B none of the four infants needed supplemental oxygen. Mean lysine, protein, glucose, fat, and energy intakes during periods A and B are presented in table 2.

Table 2

Lysine, protein, carbohydrate, fat, and energy intakes during periods A and B

Isotopic plateau

Lysine kinetics were calculated from the plateau enrichment values for plasma lysine and breath carbon dioxide (table 3). Background recovery of the [13C]label in expiratory air was not significantly different between both periods (period A −19.80 (1.28) v period B −20.24 (0.99) 13C Pee Dee Belemnite (PDB)). There was also no difference in background recovery of the [13C]label in expiratory air between the two consecutive study days of each period (A: day 1: −20.61 (1.23) v day 2: −18.99 (1.43) PDB; and B: day 1: −21.58 (0.35) v day 2: −18.89 (2.59) PDB). Although we took two blood samples after four hours of tracer infusion, we are sure that steady state was reached as we found an isotopic plateau in CO2 excretion. Before a plateau in breath is reached, a plateau has to be reached at the site of lysine oxidation (that is, intracellularly). 13CO2 enrichment in breath during [13C]sodium bicarbonate infusion rose rapidly during the first hour of infusion in both periods, to become constant in all infants by 120 minutes, with <5% variation in the plateau (period A 4.1 (1.0) v period B 3.4 (0.8)%). The coefficient of variation of breath [13C]lysine enrichment above baseline at plateau was 6.2 (1.5)% during period A and 6.8 (1.6)% during period B.

Table 3

Baseline and steady state plasma isotopic enrichments on study days 1 and 2 during period A and on study days 3 and 4 during period B

Lysine kinetics

Plasma lysine concentration was significantly higher during partial enteral feeding compared with full enteral feeding (period A 231 (108) v period B 133 (48) μmol/(kg×h); p<0.05). As expected, whole body lysine fluxes were higher for the oral than for the intravenous tracer group during both periods (period A flux intragastric 396 (49) v flux intravenous 267 (35) μmol/(kg×h); period B flux intragastric 279 (42) v flux intravenous 231 (40) μmol/(kg×h)). First pass lysine uptake as a fraction of dietary lysine intake was significantly higher during restricted enteral lysine intake (period A 32 (10) v period B 18 (7)%; p<0.05). However, splanchnic lysine uptake in absolute amounts was not significantly different during full enteral intake (period A 10 (3) v period B 18 (6) μmol/(kg×h); p = 0.17). Splanchnic tissues did not oxidise dietary lysine during partial enteral feeding or during full enteral feeding (period A −4 (23) v period B −18 (20) mol/(kg×h)).

Whole body lysine oxidation was significantly lower during full enteral feeding (fig 2). Whole body lysine oxidation accounted for 50 (19)% of total lysine intake during partial enteral nutrition whereas only 17 (3)% of total lysine intake was oxidised during full enteral feeding (p<0.05). The non-oxidative disposal of lysine (NOLD; period A 222 (36) v period B 169 (36) µmol/(kg×h)) and lysine release of protein breakdown (LRP; period A 214 (39) v period B 131 (38) μmol/(kg×h)) were not significantly different during both periods. The resulting net lysine balance was significantly higher during full enteral feeding (fig 2).

Figure 2

Whole body lysine oxidation and balance in absolute amounts during period A (n = 6) and period B (n = 4). *p<0.05.


The main purpose of this study was to measure lysine kinetics in preterm infants to gain insight into the importance of splanchnic tissues in relation to whole body lysine kinetics. In addition, we wished to quantify first pass lysine metabolism during both full enteral feeding and significantly lower enteral intake. Our data demonstrated that in preterm infants receiving full enteral feeding, whole body lysine oxidation was significantly lower and lysine balance significantly higher than in preterm infants receiving partial enteral feeding. Approximately one third of dietary lysine is removed by the splanchnic region during partial enteral feeding compared with a lower fraction (18%) during full enteral feeding. Furthermore, we could not detect any first pass lysine oxidation by splanchnic tissues in either feeding period.

It is of interest to note our results in neonatal pigs receiving a low protein diet,6 with regard to the high fractional first pass lysine uptake observed under a restricted enteral amino acid supply in preterm infants. The high splanchnic extraction as a fraction of dietary intake in preterm infants receiving partial enteral feeding is also consistent with the high values for protein turnover observed in immature rats,29 with a maximal contribution of the intestine to whole body protein synthesis attained at weaning.

A high first pass uptake may also be caused by incomplete digestion and absorption of dietary proteins and amino acids. However, studies in adults and infants have shown a high digestibility of approximately 98% of milk proteins and cereal protein.30,31 This indicates that probably all dietary milk proteins are absorbed by the intestinal mucosa and are not lost in faeces. Thus lysine that does not appear in the systemic circulation is utilised by the intestine, and is not lost via faeces. Indeed, Stoll et al have demonstrated in neonatal pigs that splanchnic tissues have high requirements for amino acids and are proving to be an important modulator of whole body amino acid availability.6 High splanchnic lysine extraction in preterm infants in their first weeks of life may be necessary to provide amino acids for their high intestinal protein synthesis during a period of rapid adaptation to enteral feeding. It is well known, for example, that the presence of nutrients in the intestinal lumen provides a marked stimulus to intestinal growth.32

In contrast with our finding in piglets, intraluminal lysine or lysine taken up by splanchnic tissues is apparently not oxidised, implying that intestinal lysine metabolism in preterm infants only includes protein synthesis. In other words, these results suggest that the premature gut has a most efficient use of the essential amino acid lysine, and plays an important role in channelling dietary amino acids to peripheral tissues.

Our second aim was to determine whole body lysine kinetics in relation to two different enteral lysine supplies. Surprisingly, in preterm infants on full enteral feeding, whole body lysine oxidation was significantly lower in comparison with partial enteral feeding. Decreased whole body lysine oxidation suggests a protein sparing mechanism during full enteral feeding in preterm infants. Consequently, lysine balance increased significantly as dietary lysine intake was augmented. In concordance with these oxidation results, plasma lysine concentration was significantly higher during partial enteral feeding. During this feeding period, amino acids were administered via both the venous circulation and the stomach, in contrast with full enteral feeding. The higher plasma lysine concentration during partial enteral feeding may be a result of our direct intravenous infusion and significantly lower first pass lysine uptake by the gut. In addition, lower whole body lysine oxidation during partial enteral feeding may have contributed to a higher plasma lysine level. Another explanation for this observation may be that total amino acid intake was nutritionally better balanced during full enteral feeding, which resulted in an improved lysine balance. In other words, it might indicate that lysine is not the first limiting essential amino acid in the diet under the conditions of partial enteral feeding. Otherwise, lysine oxidation would have been lower during partial enteral feeding.

The apparent lower whole body lysine oxidation and higher lysine balance during full enteral feeding were similar to values obtained by Duffy and Pencharz.33 Their study compared the effect of feeding route (intravenous or enteral) on protein metabolism of the neonate and concluded that orally fed neonates utilise amino acids for synthesis more efficiently than total parenterally fed infants.33 Early parenteral supply of amino acids is given to prevent protein loss but the present study clearly shows that enteral feeding is a far more efficient way of feeding preterm infants. Unfortunately, intestinal immaturity often impairs early full enteral feeding but our data suggest that maximal effort should be paid to initiate enteral feeding as soon as possible in preterm infants.34

There were some limitations in our study design. Energy intake is one of the factors that influence protein synthesis rate. Although lysine intakes during both feeding periods were almost identical, energy intake was significantly different between the feeding periods. Intake of metabolic energy by partial enterally fed infants was 74% of the intake of enterally fed infants. Parenterally fed neonates appear to require only 75% of the energy of enterally fed infants to achieve the same growth rate35 because of the lower energy expenditure of the splanchnic tissues during parenteral nutrition. In addition, enterally fed infants have some energy losses in stool due to malabsorption. In a previous study, we found no significant effect of reducing energy intake from 120 to 100 kcal/(kg×day) on nitrogen excretion and protein deposition in preterm infants.36 Van Goudoever et al showed in preterm infants that the overall pattern of fuel utilisation was unaffected by the mode of feeding.37 Therefore, we speculate that the higher energy intake during full enteral feeding did not significantly influence lysine kinetics.

Another issue relevant to the study design is the different postnatal ages at both study periods. Infants were older during period B, which may have influenced lysine kinetics. However, it was not feasible in our study design to have infants on full enteral feeding on day 7. On the other hand, it was not ethically justified to have infants on partial enteral feeding at a postnatal age of three weeks for a period of at least five days in order for them to adjust to a reduced enteral feeding rate. In addition, we wanted to evaluate lysine kinetics in preterm infants who were treated according to standard neonatal intensive care feeding regimens.

In conclusion, we have shown that splanchnic tissues have a high rate of metabolism and use almost one third of dietary lysine intake in preterm infants undergoing partial enteral feeding. However, although lysine is catabolised by the whole body to a great extent, especially during partial enteral feeding, it is not catabolised by splanchnic tissues during first pass in preterm infants. Furthermore, lysine balance was much higher during full enteral feeding compared with partial enteral feeding. Taken together, the results indicate that parenterally administered lysine is not as effective as dietary administered lysine in promoting protein deposition in preterm infants in their first weeks of life.


Lysine flux was calculated as:

Embedded Image

where Qiv is flux of the intravenous lysine tracer (μmol/(kg×h)), iL is the lysine infusion rate (μmol/kg×h)), and Ei and Ep are the enrichments (mol% excess) of [U-13C or 2H4]lysine in the lysine infusate and in plasma at steady state, respectively.

First pass lysine uptake was calculated as:

Embedded Image

where U is first pass lysine uptake (μmol/(kg×h)), Qig is flux of the intragastric lysine tracer (μmol/(kg×h)), and I is enteral lysine intake (μmol/(kg×h)).

At steady state, the amount of lysine entering the plasma pool should be equal to the amount of lysine leaving the pool. Lysine can enter the pool either by being released from proteins as result of breakdown or through diet. Lysine leaving the pool may be either oxidative disposal or non-oxidative disposal.

Embedded Image

where Q is flux of the lysine tracer (μmol/(kg×h)), LRP is the amount of lysine released from protein via protein breakdown (μmol/(kg×h)), Ox is the rate of lysine oxidation (μmol/(kg×h)), and NOLD is the rate of non-oxidative lysine disposal (μmol/(kg×h)).

Net lysine balance, an index of protein deposition, was calculated as:

Embedded Image

where B is lysine balance (μmol/(kg×h)).

Whole body CO2 production was estimated as:

Embedded Image

where iB is the infusion rate of [13C]sodium bicarbonate (μmol/(kg×h)), EiB is enrichment (mol% excess) of [13C]bicarbonate in the bicarbonate infusate and IEB is 13CO2 enrichment at plateau during the [13C]sodium bicarbonate infusion (mol% excess). This equation does not correct for retention of [13C]bicarbonate in different body pools and will overestimate CO2 production rate. However, the same correction factor has to be applied to quantify the lysine oxidation rate (eqn (6)). Consequently, lysine oxidation rate is not overestimated.38

As described previously, lysine oxidation was calculated by multiplying recovery of [13C]lysine in expiratory air with the rate of appearance of lysine.38 The fraction of lysine oxidised was measured according to the following equation, assuming a constant VCO2 (rate of CO2 production) during the study, that lasted seven hours38:

Embedded Image

where IEL and IEB are 13CO2 enrichments (mol% excess) at steady state during intravenous [U-13C]lysine and [13C]bicarbonate infusion. The iL is multiplied by a factor of 6 to account for the number of C atoms that are labelled.

Whole body lysine oxidation was then calculated as:

Embedded Image

Calculation of the metabolism of enterally administered [U-13C]lysine to CO2 is complicated by the fact that some of the lysine tracer is absorbed and thereby labels the arterial pool. Thus the non-first pass oxidation of [13C]labelled lysine that was administered enterally but was absorbed and entered the body at study day 2 can be calculated as:

Embedded Image

where IE13C ig is the 13C enrichment (mol% excess) in plasma during intragastric [13C]lysine infusion on study day 2, and IE13C iv is the 13C enrichment in plasma during intravenous [13C]lysine infusion on study day 1.

Total lysine oxidation on study day 2 is calculated as described previously:

Embedded Image

where Qiv is the flux of the intravenous [2H4]lysine tracer (μmol/(kg×h)).

First pass lysine oxidation can thus be calculated as:

Embedded Image


The Sophia Foundation of Scientific Research (Kröger Foundation) and the Royal Netherlands Academy of Arts and Science (Ter Meulen Fund) supported this work. We thank J Francke for laboratory analysis. We thank Professor Dr D Tibboel for helpful comments and review of the manuscript.


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  • This paper is dedicated to the memory of P J Reeds. His passing in late 2002 represented an enormous loss for everyone who had the privilege of working with him. Dr Reeds was a great mentor and noble role model, who continues to provide inspiration to his former colleagues.

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