To date, most concepts on the frequency of gastro-oesophageal reflux episodes and the efficiency of the antireflux barrier have been based on inferences derived from measurement of oesophageal pH. The development of intraluminal impedance monitoring has highlighted the fact that pH monitoring does not detect all gastro-oesophageal reflux events when little or no acid is present in the refluxate, even if special pH tracing analysis criteria are used. In November 2002, a workshop took place at which 11 specialists in the field of gastro-oesophageal reflux disease discussed and criticised all currently available techniques for measurement of reflux. Here, a summary of their conclusions and recommendations of how to achieve the best results from the various techniques now available for reflux measurement is presented.
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- GORD, gastro-oesophageal reflux disease
- LOS, lower oesophageal sphincter
- tLOSR, transient lower oesophageal sphincter relaxation
- DGOR, duodenogastro-oesophageal reflux
To date, most concepts on the frequency of gastro-oesophageal reflux episodes and the efficiency of the antireflux barrier have been based on inferences derived from measurement of oesophageal pH. The development of intraluminal impedance monitoring has highlighted the fact that pH monitoring does not detect all gastro-oesophageal reflux events when little or no acid is present in the refluxate, even if special pH tracing analysis criteria are used. The total rate of reflux episodes is an important indicator of the competence of the antireflux barrier and is therefore relevant when evaluating the effect of therapies directed at improving antireflux barrier function. In November 2002, a workshop took place at which 11 specialists in the field of gastro-oesophageal reflux disease (see list of contributors at the end of this article) discussed and criticised all currently available techniques for measurement of reflux. This paper presents a synthesis of their conclusions and recommendations of how to achieve the best results from the various techniques now available for reflux measurement.
It was concluded that intraluminal impedance monitoring is the only recording method that can achieve high sensitivity for detection of all types of reflux episodes while pH-metry is required for characterisation of reflux acidity. However, the role of intraluminal impedance in the management of patients with gastro-oesophageal reflux disease still needs to be defined from appropriately designed clinical trials.
The impact of new techniques on the definition of different types of reflux was also discussed extensively at the workshop, and new or slightly amended definitions are proposed—namely, “acid reflux”, “superimposed acid reflux”, “weakly acidic reflux”, and “weakly alkaline reflux”.
Gastro-oesophageal reflux disease (GORD) arises from increased exposure and/or sensitivity of the oesophageal mucosa to gastric contents. To date, most concepts on the frequency of gastro-oesophageal reflux episodes and the efficiency of the antireflux barrier have been based on inferences derived from measurement of oesophageal pH. However, pH monitoring does not detect all gastro-oesophageal reflux events even when special pH analysis criteria are used, particularly when little or no acid is present in the refluxate. This is the case in both adults and infants after eating, before the gastric contents have become acidified, and it also applies to reflux in patients undergoing antisecretory therapy. Not only the acidity but also the air-liquid composition of the refluxate could be relevant in the pathogenesis of GORD.
The total rate of reflux episodes is an important indicator of the competence of the antireflux barrier and is therefore relevant when evaluating the effect of therapies directed at improving the antireflux barrier function.1 Furthermore, oesophageal or extraoesophageal symptoms of GORD may be related to less acidic2–4 or gas reflux that is not detected by pH-metry.
“Oesophageal or extraoesophageal symptoms of GORD may be related to less acidic or gas reflux that is not detected by pH-metry”
Other methodologies have evolved to complement ambulatory pH monitoring for the detection and characterisation of gastro-oesophageal reflux. Intraluminal electrical impedance offers the potential to detect and monitor liquid or air movement within the oesophageal lumen,5 and Bilitec, a spectrophotometric method, can detect the presence of bilirubin in the refluxate.6 These new techniques have enabled more precise evaluation of GORD and offer the opportunity to conceive gastro-oesophageal reflux more broadly, both in terms of frequency and characteristics of the refluxate. As a consequence, new definitions of reflux and consistent terminology within the literature are required.
An international workshop that involved 11 experts working in the field of GORD was held in Oporto, Portugal, November 2002. The aims of this workshop were: (1) to review and discuss critically the performance of the various tools currently available for detection of gastro-oesophageal reflux events; and (2) to propose consensus based definitions of acid, non-acid, and gas reflux, applicable to both the adult and paediatric populations.
TECHNICAL ASPECTS OF GASTRO-OESOPHAGEAL REFLUX DETECTION (TABLE 1)
Oesophageal pH-metry consists of a continuous recording of pH in the distal oesophagus. Detection of periods of oesophageal acidification allows for a direct diagnosis of episodes of gastro-oesophageal reflux and quantification of the exposure of the distal oesophagus to acid.7 This technique is frequently presented as a gold standard for the diagnosis of GORD and for clinical pharmacology studies.8
The basic equipment requirements are a portable data logger for data storage, a pH electrode, a computer, and software for analysis of pH data. The main characteristics expected from the data logger are a frequency of sampling of the pH signal adjustable and great enough to detect short lasting falls in pH, and the availability of several event markers to signal symptoms and other events during the recording. The pH electrode should be stable, with a fast and linear response time. The pH probe measures acidity only at the level of the pH sensor. A pH of 4 corresponds to 0.12 mEq H+/l.9 The pH measurement gives no indication of the volume of the refluxate—that is, several litres of gastric contents with a pH 2 appears the same to the pH probe as a few millilitres of the same material. Routine clinical pH measurement is performed with one distal pH sensor and does not allow characterisation of the proximal extent of the refluxate. Experimental studies using multiple pH sensors permit evaluation of the proximal extent of acid reflux.10.
Sensitivity of pH monitoring
The sensitivity of pH monitoring to detect individual acid reflux episodes is determined by sampling frequency, duration threshold, pH threshold,11 and the recurrence of reflux prior to pH recovery. Sampling frequency does not affect measurement of per cent time that pH is below 4 but it does affect the number of reflux events detected when the sampling frequency is too low (table 1). When greater than 1 Hz, the sampling frequency becomes optimal (fig 1).12 Short lived pH drops may represent real reflux1 or a loss of pH signal (which can be caused by air passing the pH sensor). The prevalence and clinical significance of short lived reflux events is currently unknown. For routine pH-metry, most equipment uses 0.25 Hz8 but it was agreed that when a pH data logger digitisation frequency of less than 1 Hz is used, the sensitivity of pH monitoring for recognition of reflux episodes will be impaired in proportion to the actual digitisation frequency.
Specificity of pH monitoring
False positive counting of reflux episodes may be due to acidic food ingestion or non-reflux related fluctuations in pH of approximately 4 due to body movement, respiration, or electrode drift. Suggested strategies to improve specificity included the elimination of meals from scored intervals, defining a necessary increment of pH drop, and setting a recovery time for the pH to return to a value greater than pH 4 (table 1). However, these modifications would also affect the sensitivity of the method.
Stationary oesophageal manometry is performed either by water perfused assemblies connected to external transducers or by solid state catheters in which the microtransducers are built into the catheter itself. For prolonged measurement of lower oesophageal sphincter (LOS) pressure, perfused and solid state specialised sensors have been developed.13 The perfused sleeve sensor is widely used to measure LOS relaxations associated with gastro-oesophageal reflux.14–16
Using manometric parameters, Wyman et al judged that gastro-oesophageal reflux had definitely occurred with the concurrent finding of absent LOS pressure and a common cavity phenomenon that ended by primary or secondary peristalsis.1
A common cavity phenomenon consists of an abrupt increase in oesophageal body pressure to intragastric pressure level, observed in at least two distal oesophageal recording sites and persisting until the subsequent occurrence of either primary or secondary peristalsis (fig 2).15 For this phenomenon to occur, there should be relaxation and opening of both the LOS and crural diaphragm,17 and the refluxate should be of sufficient volume to envelop the distal oesophageal recording sites.
“In neonates and infants, common cavities occur during a substantially higher proportion of reflux episodes than in adults”
It was proposed at the workshop that measurement of common cavities could add secondary information on the mechanical features of individual reflux episodes but there was no consensus of agreement on this point. In neonates and infants, common cavities occur during a substantially higher proportion of reflux episodes than in adults.18 This is probably due to the fact that in this population the reflux volume relative to the luminal volume is greater and extends higher into the oesophagus.19
Sensitivity of manometry
Although common cavities are a manometric pattern clearly associated with liquid and gas reflux,9,20 they are a relatively insensitive measure of reflux. Acid reflux may occur without a common cavity if there is a refluxate of diminutive volume or a very low gastro-oesophageal pressure gradient, making it technically difficult to detect the common cavity. In a recent study by Shay and Richter, designed to assess the sensitivity of manometry, pH-metry, and impedance for detection of gastro-oesophageal reflux, manometry detected 76% of almost 1000 reflux episodes.21
Specificity of manometry
A common cavity pattern can be obtained by intraoesophageal insufflation without communication between the oesophagus and stomach. Therefore, it is a marker of oesophageal distension with gas or liquid rather than specifically of gastro-oesophageal reflux.
Intraluminal impedance monitoring
The term “intraluminal impedance monitoring” should be taken to include the concurrent measurement of impedance from multiple intraluminal recording segments. This method allows detection of gastro-oesophageal reflux based on changes in resistance to electrical current flow between two electrodes, when a liquid and/or gas bolus moves between them.5
“The term “intraluminal impedance monitoring” should be taken to include the concurrent measurement of impedance from multiple intraluminal recording segments”
The impedance catheter, with mounted cylinder shaped metallic electrodes, is swallowed and positioned within the oesophageal body. The impedance (or resistance) to the current flow delivered between two electrodes depends on the electrical conductivity of the environment surrounding the electrodes (luminal content, mucosa, wall thickness) as well as the cross sectional area. When a liquid bolus with high electrical conductivity bridges two electrode rings, impedance decreases. In contrast, a gas bolus with very low electrical conductivity increases impedance. Furthermore, opening of the lumen by a bolus passing along the measuring segment results in a drop in impedance whereas luminal closure causes impedance to increase.5 The difference in patterning of the electrical conductivity of liquid, gas, or mixed (liquid-gas) intraluminal content allows distinction among these luminal contents whereas the sequence of impedance changes in different segments allows recognition of flow in either aboral (swallow related) or oral (reflux) directions. Impedance does not measure the acidity of the intraluminal content.22
Gastro-oesophageal reflux is detected as an orally progressing drop in impedance, starting at the level of the LOS and propagating to more proximal impedance measuring segments (fig 3).19 Previous studies and an experimental validation study in cats20,23 using simultaneous impedance and fluoroscopy showed that liquid reflux produced an impedance drop in the oesophagus of 46.5% from baseline values, with the result that subsequent studies used drops of 50–60% in impedance to declare the presence of liquid within the oesophagus.9,19,20,24–29 Most participants felt that the 50% reduction was too stringent and that the pattern of reflux was as important. Impedance is very sensitive for detection of small volumes of liquid, and similar drops in impedance are observed with boluses of 1 ml and 10 ml.30 Therefore, to date, the intraluminal impedance technique cannot estimate the volume of the refluxate.
Intraluminal air (which has a very low electrical conductivity) provokes a rapid (3 kΩ/s) and pronounced rise in impedance.20 In the absence of swallowing, gastro-oesophageal reflux of gas is detected as an almost simultaneous or rapidly orally progressing rise in oesophageal impedance in at least two oesophageal impedance channels.10,20 In the upright position, intraluminal air is better detected by impedance recordings in the most proximal oesophagus.
Location of impedance electrodes
For reliable recognition of reflux episodes, it was agreed that the centre of the most distal impedance channel should be 1–2 cm above the proximal margin of the LOS (table 1). This location allows recognition of the so-called short segment reflux—that is, reflux restricted to the very distal oesophagus that does not reach 5 cm above the LOS.10,20,31
When there is an abnormally low baseline intraluminal impedance in the most distal oesophageal recording segment (for example, in patients with oesophagitis),32 the impedance drop recorded by this may not be sufficient to reach the defined threshold. It is possible to use the more proximal channels however and a liquid reflux episode is proven when there is an abrupt reduction in impedance in the next two adjacent recording segments. It was agreed therefore that reliable recognition of reflux episodes requires impedance recording from three adjacent 2 cm long segments in the distal oesophagus.
“During gastro-oesophageal reflux, drops in impedance in the mid and upper oesophagus indicate the proximal extent of the liquid refluxate”
During gastro-oesophageal reflux, drops in impedance in the mid and upper oesophagus indicate the proximal extent of the liquid refluxate.33,34 So far there are no studies that have compared the proximal extent of reflux measured with impedance with data on this from other techniques (that is, scintigraphy or radiology).
Sensitivity of impedance monitoring
The sensitivity of impedance has been determined by comparing its ability to detect reflux with that of manometry and pH-metry. In fasting recumbent patients with severe GORD, impedance detected 95% of 408 reflux events diagnosed by combined manometry and pH-metry.9,34 Compared with manometry, impedance detected reflux in 92–99% of common cavities.9 Compared with pH-metry, 97–98% of acid reflux events were detected by impedance under both stationary and ambulatory conditions.27,28 From published evidence, it was concluded that an optimal arrangement of impedance electrodes in adults has a sensitivity of at least 90% for detection of all reflux episodes (table 1). The sensitivity of impedance for reflux recognition may be affected by the occurrence of very low baseline impedance values, such as may be observed in the presence of oesophagitis (fig 4). Preliminary studies have found that impedance changes due to acute oesophagitis are reversed when this heals.35
While the presence of oesophagitis does not preclude the recognition of reflux during visual analysis, it can make automated recognition difficult. Another cause of low basal impedance is the presence of Barrett’s columnar epithelium (table 1). There is a reasonable correlation between the length of the columnar epithelium, as measured by impedance, and endoscopy.32
Specificity of impedance monitoring
During combined impedance-pH-manometric studies, false positive impedance changes (that is, a typical impedance retrograde flow reflux pattern without any pH or manometric suggestion of reflux) were rare (table 1). One source of error is swallow related drops in impedance.34 False negative impedance (that is, pH drops without impedance change) can be observed during slow pH drifts or during reflux events that occur soon after swallowing.
Bilitec is a monitoring system that can detect duodenogastro-oesophageal reflux (DGOR) by utilising the optical properties of bilirubin (table 1).36–41 Although Bilitec does not measure concentrations of duodenal components, a good correlation has been found between bilirubin content and the concentrations of pancreatic enzymes in aspirated refluxate, suggesting that bilirubin is a good tracer for duodenal components in the gastro-oesophageal refluxate.36,37 The working principle of Bilitec is that detection in the oesophageal lumen of any absorption near 450 nm suggests the presence of bilirubin, and therefore DGOR. There was a consensus therefore that the term “oesophageal bilirubin monitoring” should be applied to the use of continuous measurement of bilirubin concentration for recognition of reflux of duodenal contents into the oesophagus.
“Bilitec is a monitoring system that can detect duodenogastro-oesophageal reflux (DGOR) by utilising the optical properties of bilirubin”
DGOR data measured by Bilitec are usually presented as “% time bilirubin absorbance is higher than 0.14”(table 1). This cut off value has been selected because studies have shown that values below this number could be due to suspended particle and mucus present in the gastric contents.36,37
Sensitivity of bilirubin measurement
The sampling rate of the Bilitec probe is only once per eight seconds. The software averages between the absorbencies calculated over two successive samples to decrease the noise of the measurements.37 This is a very low sampling frequency compared with pH monitoring and suggests that this technique is unable to detect with accuracy the time of onset of bilirubin reflux into the oesophagus. However, there are no data comparing the number of DGOR events at different sampling frequencies.
Specificity of bilirubin measurement
The spectrophotometric capsule has a gap that is traversed by the measuring light beam. Solid material can be trapped in this gap and so interfere with the function of the sensor. Consistent with this, several studies have demonstrated the need to use a liquid special diet, as major meal artefacts, thought to be due to food being trapped in the capsule gap, are observed in up to 20% of the recordings where a solid diet has been used.42 A liquid diet also allows better correlation with simultaneous pH monitoring.43 It was agreed that it is impossible for bilirubin monitoring with the Bilitec apparatus to reflect with accuracy the number of DGOR episodes because of the several technical limitations in the functioning of this equipment (table 1).
REFLUX SUBCATEGORIES (TABLE 2)
To date, studies reported in the literature7,10,11 have used different definitions for acid reflux. Acid reflux is diagnosed by pH-metry. There was strong consensus agreement among the group that acid reflux should be defined as “reflux episodes that decrease oesophageal pH across 4, or reflux that occurs when oesophageal pH is already below 4” (table 2). A caveat has to be applied to the above definition of acid reflux however as a substantial proportion of pH drops to below 4 are not due to gastro-oesophageal reflux (fluctuations of pH around 4 due to movement, respiration, or electrode drift). It should be possible to increase the sensitivity of pH measurement by increasing the sampling frequency12 and by removing duration as a criterion of reflux. Additionally, increasing the pH threshold from 4 to 5 almost doubles the number of reflux events detected. The specificity of pH measurement may be improved by setting a minimum interval between events (10 seconds), minimum duration of events (three seconds), minimum drop in pH (1 pH unit), minimum pH drop rate (0.5 pH units per second), or the hysteresis criterion (for example, fall to 3.8, rise to 4.2). It was also suggested that the specificity of pH measurement could be improved if the pH recovery level was increased to 5 instead of 4, as many non-reflux related fluctuations in pH occur at around pH 4.44 The use of combined pH monitoring and impedance measurement would permit detection of acid reflux episodes and would also obviate the need to use duration criteria, as the impedance criteria define whether or not reflux has occurred.
The occurrence of reflux episodes when the basal oesophageal pH is already below 4 was discussed in some detail. These episodes represent a special category of acid reflux and consensus was obtained that they should have a unique descriptor. This type of reflux was previously described as “re-reflux”.1,45 However, this name implies that reflux occurred, it was cleared, and that the same material was then refluxed again. It was suggested that this type of acid reflux should be renamed more accurately as “superimposed acid reflux”, as it represents the occurrence of a further reflux episode before the contents of the oesophagus have been cleared from the previous reflux episode (table 2). This is an important phenomenon underlying the delay in oesophageal clearance, particularly in patients with a hiatal hernia.46,47 It was agreed that even if optimal pH criteria and digitisation frequencies were used, pH-metry would have a low sensitivity and a low specificity for the recognition of “superimposed acid reflux” (fig 3). As many as 33% of superimposed acid reflux events (detected by manometry) are associated with no change in pH.1 Conversely, it is also known that a number of pH drops occurring when the basal oesophageal pH is below 4 (which would be classified on the basis of pH as “superimposed” reflux) are due to fluctuations in pH due to movement, respiration, or electrode drift and are not actually due to reflux. Impedance monitoring is able to detect “superimposed” reflux episodes where no change in pH occurs or when the fall in pH is <0.5 units.9
Weakly acidic reflux
If an acid reflux episode is defined as a drop in pH across 4 for at least four seconds, it is likely that the number of reflux episodes will be underestimated by approximately 50% during both the interdigestive and first three postprandial hours in adults.1,48 Additionally, when these pH criteria are applied to neonates and infants, the high frequency of feeds would result in even more reflux episodes (>66%) not causing a drop in oesophageal pH across 4 (fig 5).18
The terminology used to describe reflux episodes in which the nadir pH lies between 4 and 7 was discussed in some detail (table 2). In cases where the pH falls by at least 1 unit, but does not fall below 4, it was decided to accept the term “weakly acidic reflux” and to reject the old terminology of “minor or low acid reflux”. The upper limit of pH 7 was chosen to define the limit of weakly acidic events, as any fall below pH 7 contains some acid. Some experts felt however that detectable pH falls usually start from pH 6.5, and so consensus agreement was not obtained on this point.
The use of combined pH monitoring and impedance measurement would permit more accurate detection of weakly acidic reflux episodes.
The literature contains different definitions of the term “non-acid” reflux. It has been used to refer to: (a) reflux episodes diagnosed by manometry or scintigraphy without pH drops across 449; (b) DGOR events diagnosed with Bilitec monitoring50; (c) reflux events diagnosed by impedance monitoring with no change in pH or a drop in pH that does not reach 428; and (d) reflux events diagnosed by impedance monitoring with no change in pH or pH fall of less that 1 pH unit.20
It was decided that definitions should be based on the pH of the refluxate, as defined chemically. A pH of 7 should be the cut off between “weakly acidic” and “weakly alkaline reflux” (table 2, fig 6).
In many cases, the basal oesophageal pH is between 5 and 6 and a reflux event detected by impedance does not change the oesophageal pH. This is frequently the case in infants (fig 7). It was suggested that it would be wrong to label these types of reflux as “non-acid”, as the refluxate is weakly acidic, even though it does not decrease the previous oesophageal pH.
“Based on the pH of gastric contents, the majority of reflux episodes constitute weakly acidic reflux”
In infants and neonates, gastric pH is often higher than oesophageal pH, and therefore it is difficult to define the type of reflux episode.18 The question arises as to whether a reflux episode should be defined as “non-acid reflux” because it increases oesophageal pH, or “weakly acidic reflux” as the refluxate content is still acidic. In neonates, generally, oesophageal pH does not rise as high as 7 and therefore it would not strictly meet the new definition of “weakly alkaline reflux”. Participants felt strongly however that terminology proposed by the consensus report should be applicable to both adults and neonates and it was decided that the term “weakly alkaline reflux” should be reserved for reflux episodes during which nadir oesophageal pH does not drop below 7 (table 2). Therefore, the previous descriptor “non-acid reflux” can be renamed as “weakly alkaline reflux” that by definition is a very exceptional event. Based on the pH of gastric contents, the majority of reflux episodes constitute weakly acidic reflux.
Bilirubin monitoring provides a different approach from the other methods of reflux monitoring as it is concerned with the chemical composition of the refluxate. It detects bilirubin in the refluxate, which indicates that DGOR has occurred. A bilirubin reflux event is defined as an increase in bilirubin absorbance to above 0.14, as recorded by Bilitec, and is considered to be terminated when the absorbance value returns to less than 0.14.37
Consensus was obtained that the term “oesophageal bilirubin monitoring” should be applied to the use of continuous measurement of bilirubin concentration for recognition of reflux of duodenal contents into the oesophagus. Impedance and pH-metry can detect acid and non-acid reflux but cannot describe its composition. In spite of its technical limitations, oesophageal bilirubin concentration monitoring adds information about the chemical nature of acid and non-acid material that refluxes into the oesophagus (fig 8).
Oesophageal reflux can now be defined both in terms of its acidity and its physical state (liquid-gas). gastro-oesophageal reflux The advantages of using impedance monitoring to differentiate between liquid and gas reflux were described above (see “Technical aspects of gastro-oesophageal reflux detection” above). “Liquid reflux” is defined by the pattern of an orally progressing drop in impedance of at least 50% in at least three adjacent distal oesophageal recording segments. Provided that only reflux can cause such a typical aborally progressive pattern of rapid drops in impedance, a revised threshold would be acceptable. If these drops are recorded from electrode pairs in the mid and upper oesophagus, this indicates the extent of travel of liquid up the oesophagus. Gas reflux, on the other hand, results in a pattern of abrupt retrograde or simultaneous increases in impedance over any two segments, being independent of swallowing.
Mixed reflux of gas and liquid is more frequent than pure liquid reflux, both in healthy subjects and in patients with GORD.33 When gas and liquid are present in the refluxate, the impedance increase (indicative of gas) can precede the impedance drop (indicative of liquid), or vice versa. Whether the gas-liquid or liquid-gas sequence represents small amounts of liquid in a large volume of gas, small amounts of gas in a large volume of liquid, or a fine mix of liquid and gas cannot be determined without another, more sensitive, marker of gas reflux as a gold standard.
Impedance monitoring is the only recording method that can achieve high sensitivity for detection of all types of reflux episode. Even with optimisation of methods, the sensitivity and specificity of pH monitoring will not approach that of impedance monitoring for recognition of occurrence of individual reflux episodes.
Reflux is best detected by impedance and its acidity characterised by pH-metry. A small percentage of acid reflux events may occur as slow pH drops that are not detected by impedance, suggesting that the combination of both techniques is better than pH-metry or impedance monitoring alone. Therefore, combined pH and intraluminal impedance monitoring allows detection of all reflux events and gives the best possible evaluation of the function of the antireflux barrier. Bilirubin measurement is useful to determine the presence of duodenal content in the refluxate but the current technique is limited in its ability to detect the onset or frequency of individual DGOR events.
“Reflux is best detected by impedance and its acidity characterised by pH-metry”
The different available techniques for reflux monitoring offer the opportunity to conceive gastro-oesophageal reflux more broadly, both in terms of frequency and characteristics of the refluxate. The criteria developed for their use apply to both adults and children or neonates and these techniques may be used alone or in combination, according to the clinical scenario. The decision to add impedance to pH monitoring should be determined by the primary aim of the particular investigation. For example, in diagnostic studies where patients with GORD are “off” antisecretory therapy, pH-metry alone may be sufficient in the majority of cases. However, in adult patients with GORD and persistent symptoms despite adequate therapy, or in neonates, combined pH-impedance monitoring may prove superior. To date, the lack of automated analysis remains a significant limitation of impedance monitoring. However, algorithms for automated analysis of prolonged pH-impedance monitoring are currently under development. Clinical studies and outcome data will ultimately define the usefulness of these new techniques and definitions for reflux monitoring.
We would like to thank Dr Madeline Frame for assistance with the preparation of this manuscript. The workshop was supported by an educational grant from Astra Zeneca
Professor L Akkermans, Department of Surgery, University Hospital, Utrecht PO Box 8500, 3508 GA Utrecht, the Netherlands; Professor D Castell, Director, Esophageal Disorders Program, Medical University of South Carolina, 96 Jonathan Lucas St STE 210, PO Box 250327, Charleston SC 29625, USA; Professor G Davidson, Centre for Paediatric and Adolescent Gastroenterology, Women’s and Children’s Hospital and Department of Paediatrics, University of Adelaide, North Adelaide, Australia; Professor J Dent, Department of Gastroenterology, Hepatology, and General Medicine, Level 7 North Wing, Royal Adelaide Hospital, North Terrace, Adelaide, South Australia 5000; Associate Professor R Holloway, Department of Gastroenterology, Hepatology, and General Medicine, Level 7 North Wing, Royal Adelaide Hospital, North Terrace, Adelaide, South Australia 5000; Professor PJ Kahrilas, Northwestern University, The Feinberg School of Medicine, Division of Gastroenterology, 676 North St Clair St, Suite 1400, Chicago, Illinois 60611-2951, USA; Dr T Omari, Centre for Paediatric and Adolescent Gastroenterology, Women’s and Children’s Hospital and Department of Paediatrics, University of Adelaide, North Adelaide, Australia; Dr S Shay, Gastroenterology Service, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA; Associate Professor D Sifrim, Center for Gastroenterological Research, Department of Gastroenterology, University Hospitals KU Leuven, Herestraat 49, B-3000 Leuven, Belgium; Professor A Smout, Academisch Ziekenhuis Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the Netherlands; Professor J Tack, Center for Gastroenterological Research, Department of Gastroenterology, University Hospitals KU Leuven, Herestraat 49, B-3000 Leuven, Belgium.
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