Identification of a genetic marker of Helicobacter pylori strains involved in gastric extranodal marginal zone B cell lymphoma of the MALT-type
- P Lehours1,
- S Dupouy1,
- B Bergey1,
- A Ruskoné-Foumestraux2,
- J C Delchier3,
- R Rad4,
- F Richy1,
- J Tankovic3,
- F Zerbib1,
- F Mégraud1,
- A Ménard1
- 1Laboratoire de Bactériologie, Université Victor Segalen Bordeaux 2, Bordeaux, France
- 2Service de Gastroentérologie, Hôpital Hôtel-Dieu, AP-HP, Paris, France
- 3Service d’Hépatologie-Gastroentérologie, Hôpital Henri Mondor, Créteil, France
- 4Department of Internal Medicine II and Gastroenterology, Bogenhausen Academic Teaching Hospital, Technical University of Munich, Munich, Germany
- Correspondence to:
Dr A Ménard
Université Victor Segalen Bordeaux 2, Laboratoire de Bactériologie, Bat 2B RDC Zone Nord, 33076 Bordeaux Cedex, France;
- Accepted 7 January 2004
Background and aims: Gastric extranodal marginal zone B cell lymphoma of the mucosa associated lymphoid tissue (MALT)-type (MZBL) is a rare complication of Helicobacter pylori infection. Currently, no bacterial factor has been associated with the development of this disease. Our aim was to identify genes associated with lymphoma development.
Methods: We used subtractive hybridisation as a tool for comparative genomics between H pylori strains isolated from a patient with gastric MZBL and from a patient with gastritis only.
Results: When gastric MZBL strains were compared with gastritis strains, two open reading frames (ORFs) were significantly associated with gastric MZBL: JHP950 (74.4% v 48.7%, respectively; p = 0.023) and JHP1462 (25.6% v 2.6%, respectively; p = 0.004). The prevalence of JHP950 was 48.8% (p = 0.024) in duodenal ulcer strains and 39.3% (p = 0.006) in gastric adenocarcinoma strains, which makes this ORF a specific marker for gastric MZBL strains. In contrast, the prevalence of JHP1462 was 16% (p = 0.545) and 35.7% (p = 0.429) in duodenal ulcer and adenocarcinoma strains, respectively. These ORFs were present in reference strain J99 but not in reference strain 26695. JHP950 is located in the plasticity zone whereas the other, JHP1462, is located outside. Both encode for H pylori putative proteins with unknown functions.
Conclusion: Despite its low prevalence, the ORF JHP1462 can be considered a candidate marker for H pylori strains involved in severe gastroduodenal diseases. In contrast, the ORF JHP950 has a high prevalence, and is the first candidate marker for strains giving rise to an increased risk of gastric MZBL strains. Further confirmation in other studies is needed.
- RT-PCR, reverse transcription-polymerase chain reaction
- MALT, mucosa associated lymphoid tissue
- gastric MZBL, gastric extranodal marginal zone B cell lymphoma of the MALT-type
- MCA, multiple correspondence analysis
- ORF, open reading frame