Nuclear factor κB inactivation in the rat liver ameliorates short term total warm ischaemia/reperfusion injury
- H Suetsugu1,
- Y Iimuro2,
- T Uehara1,
- T Nishio1,
- N Harada1,
- M Yoshida1,
- E Hatano1,
- G Son2,
- J Fujimoto2,
- Y Yamaoka1
- 1Department of Gastroenterological Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan
- 2First Department of Surgery, Hyogo College of Medicine, Nishinomiya, Japan
- Correspondence to:
Dr Y Iimuro
First Department of Surgery, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan;
- Accepted 9 November 2004
- Revised 7 October 2004
Background: In hepatic ischaemia/reperfusion injury, activated liver macrophages (Kupffer cells) are dominantly regulated by a transcription factor, nuclear factor κB (NFκB), with respect to expression of inflammatory cytokines, acute phase response proteins, and cell adhesion molecules.
Aims: We assessed whether inactivation of NFκB in the liver could attenuate total hepatic warm ischaemia/reperfusion injury.
Methods: We studied rats with hepatic overexpression of inhibitor κBα super-repressor (IκBα SR) caused by a transgene introduced using an adenoviral vector. Hepatic ischaemia/reperfusion injury was induced under warm conditions by total occlusion of hepatoduodenal ligament structures for 20 minutes, followed by reperfusion. Controls included uninfected and control virus (AdLacZ) infected rats.
Results: IκBα SR was overexpressed in Kupffer cells as well as in hepatocytes, blocking nuclear translocation of NFκB (p65) into the nucleus after reperfusion. Gene transfection with IκBα SR, but not with LacZ, markedly attenuated ischaemia/reperfusion injury, suppressing inducible nitric oxide synthase and nitrotyrosine expression in the liver. Moreover, no remarkable hepatocyte apoptosis was detected under IκBα SR overexpression.
Conclusions: Adenoviral transfer of the IκBα SR gene in the liver ameliorates short term warm ischaemia/reperfusion injury, possibly through attenuation of hepatic macrophage activation.
- NFκB, nuclear factor κB
- IκB, inhibitor κB
- IκBα SR, IκBα super-repressor
- ROS, reactive oxygen species
- TNF-α, tumour necrosis factor α
- HA, haemagglutinin
- AST, aspartate aminotransferase
- ALT, alanine aminotransferase
- LDH, lactate dehydrogenase
- iNOS, inducible nitric oxide synthase
- HNE, 4-hydroxy-2′-nonenal
- RT-PCR, reverse transcriptase-polymerase chain reaction
- NPC, non-parenchymal liver cells
- ssDNA, single stranded DNA
Conflict of interest: None declared.