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Rectal sensorimotor dysfunction in patients with urge faecal incontinence: evidence from prolonged manometric studies
  1. C L H Chan1,
  2. P J Lunniss1,
  3. D Wang2,
  4. N S Williams1,
  5. S M Scott1
  1. 1Centre for Academic Surgery, Barts and the London, Queen Mary’s School of Medicine and Dentistry, London, UK
  2. 2Medical Statistics Unit, London School of Hygiene and Tropical Medicine, London, UK
  1. Correspondence to:
    Dr S M Scott
    Centre for Academic Surgery, GI Physiology Unit, 3rd Floor Alexandra Wing, Royal London Hospital, Whitechapel, London E1 1BB, UK; m.scottqmul.ac.uk

Abstract

Background and aims: Although external anal sphincter dysfunction is the major cause of urge faecal incontinence, approximately 50% of such patients have evidence of rectal hypersensitivity and report exaggerated stool frequency and urgency. The contribution of rectosigmoid contractile activity to the pathophysiology of this condition is unclear, and thus the relations between symptoms, rectal sensation, and rectosigmoid motor function were investigated.

Methods: Fifty two consecutive patients with urge faecal incontinence, referred to a tertiary surgical centre, and 24 volunteers, underwent comprehensive anorectal physiological investigation, including prolonged rectosigmoid manometry. Patients were classified on the basis of balloon distension thresholds into those with rectal hypersensitivity (n = 27) and those with normal rectal sensation (n = 25). Automated quantitative analysis of overall rectosigmoid contractile activities and, specifically, high amplitude contractions and rectal motor complex activity was performed.

Results: External anal sphincter dysfunction was similar in both patient groups. Overall, phasic activity and high amplitude contraction frequency were greater, and rectal motor complex variables significantly altered, in those with rectal hypersensitivity. Symptoms, more prevalent in the rectal hypersensitivity group, were also more often associated with rectosigmoid contractile events. For individuals, reduced compliance and increased rectal motor complex frequency were only observed in patients with rectal hypersensitivity.

Conclusions: We have identified a subset of patients with urge faecal incontinence—namely, those with rectal hypersensitivity—who demonstrated increased symptoms, enhanced perception, reduced compliance, and exaggerated rectosigmoid motor activity. Comprehensive assessment of rectosigmoid sensorimotor function, in addition to evaluation of anal function, should be considered in the investigation of patients with urge faecal incontinence.

  • ANOVA, analysis of variance
  • AUC, area under the curve
  • CS, control subjects
  • EAS, external anal sphincter
  • DDV, defecatory desire volume
  • HAC, high amplitude contraction
  • HADS, hospital anxiety and depression scale
  • HAPC, high amplitude propagated contraction
  • IAS, internal anal sphincter
  • IBS, irritable bowel syndrome
  • MTV, maximum tolerable volume
  • NS, normal rectal sensation
  • PNTML, pudendal nerve terminal motor latency
  • RH, rectal hypersensitivity
  • RMC, rectal motor complex
  • SCID, structured and clinical interview for DSM-III-R
  • UFI, urge faecal incontinence
  • rectal sensorimotor dysfunction
  • urge faecal incontinence
  • manometry

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Maintenance of bowel continence involves coordination between anorectal and colonic function, and psychobehavioural factors. Faecal incontinence is a major cause of social and psychological disability, reported in approximately 2% of the adult population,1 although this likely represents an underestimate as sufferers are often reluctant to volunteer such symptoms.2,3 Pathophysiology may be multifactorial.4 Urge faecal incontinence (UFI), where incontinent episodes occur against the patient’s will, due to lack of voluntary control, is the most common presenting symptom.4 External anal sphincter (EAS) dysfunction, either secondary to compromised structural integrity, neurological injury, or a combination of both, is recognised as the major cause of UFI.5–7 Nevertheless, patients with an anatomically intact and normal functioning EAS also experience episodes of UFI,8 indicating that other pathophysiological mechanisms may contribute to symptoms. It is known that alterations in suprasphincteric mechanisms influence continence9 but their precise role in UFI remains undetermined. The reservoir function of the colorectum may be compromised, for example, by disturbance of sensorimotor function.10–12 Furthermore, as visceral sensory and motor mechanisms of the anorectum and colon are themselves inextricably linked, alterations in the motor component may effect change in sensory function, and vice versa.13 This interaction may be further modulated and modified by higher cortical centres.14

Evaluation of rectal sensory function in patients with UFI has demonstrated that up to ∼50% of patients have evidence of rectal hypersensitivity (RH) to simple volumetric balloon distension—that is, reduced sensory thresholds.5,15 We have recently shown that in patients with UFI, RH is associated with increased bowel frequency, reduced ability to defer defecation, increased pad usage, and negative lifestyle effects.5

Motor function of the colon and rectum is an integrated process involving myoelectrical and contractile activity, tone, compliance, wall tension, and stool transit.16 Although some information is available regarding the influence of alterations in certain motor components, such as compliance,10–12,17 tone,18 and transit19 in UFI, little is known about the contribution of colorectal contractile activity to the pathophysiology of this condition. Numerous studies have used prolonged ambulatory manometry to investigate colonic contractile activity in normal subjects and patients with constipation,19–23 but few studies have used this technique to address possible colorectal dysmotility in faecal incontinence.19,24 Colorectal motility comprises a number of distinct phasic contractile activities, both isolated and in recognisable patterns.25 Two components, high amplitude contractions and rectal motor complexes (RMC), have been shown to be functionally important in patients with faecal incontinence. In one study, a strong correlation between the urge to defecate (represented by depression of an event marker) and high amplitude propagated contractions (HAPC) was demonstrated in both healthy individuals and in the six patients with faecal incontinence.19 All episodes of urge incontinence were associated with propagation of HAPCs from the transverse colon to the rectum.19 HAPCs are the major motor correlates of mass intraluminal movement,26 and there is a clear association with both the urge to defecate22,27 and faecal expulsion.28,29 In a separate study, Santoro et al showed RMC frequency to be increased in a proportion of patients with idiopathic faecal incontinence.24 The RMC is a subconscious intrinsic motor programme comprising regular cyclical bursts of phasic pressure waves.19,23,30–32 The function of the RMC remains unclear but is thought to represent localised segmental activity.31,32 It now appears that the RMC, although predominant in the rectosigmoid region, is indeed manifest throughout the colon, where it has been termed the colonic motor complex.20,23,31,32 Like the small bowel migrating motor complex, which has been characterised to a much greater extent over the past three decades,16,33 it has been proposed that the RMC may also be used as a marker of enteric neuromotor function, as its presence is independent of intact extrinsic innervation.34–36 Prolonged ambulatory manometry, which is now a well recognised clinical tool for the investigation of small intestinal dysmotility,37,38 as well as a research tool for the study of colorectal motility,25 has demonstrated that both qualitative and quantitative abnormalities of cyclical motor activity may be of pathological significance.21,38–40

The primary aim of this study was to evaluate rectosigmoid motor activity over a prolonged period in a large cohort of patients with the specific symptom of UFI. The secondary aim was to investigate the relationships between rectal sensation and colorectal motor function, to test the hypothesis that the exaggerated symptoms observed in patients with rectal hypersensitivity are associated with differences in contractile activity.

MATERIALS AND METHODS

Subjects

Patients with urge faecal incontinence (UFI)

The study population consisted of 52 patients with UFI (38 females; median age 45.5 years (range 18–72)) referred consecutively to a tertiary surgical coloproctology centre. All patients had a detailed clinical history taken and underwent investigations to exclude organic gastrointestinal pathology. From their histories, details regarding symptom onset, bowel frequency, and frequency of incontinence episodes were recorded. All patients also underwent a structured clinical interview (SCID) for DSM-III-R (Diagnostic and Statistical Manual of Mental Disorders) to screen for psychopathology41 and completed validated screening questionnaires: the bowel disease questionnaire42 and the hospital anxiety and depression scale (HADS).43 These questionnaires were used to identify the presence of the irritable bowel syndrome (IBS), as defined by the Rome II criteria,44 and anxiety or depressive disorders. A score of up to 7 in either the anxiety or depression scale of the HADS is regarded as normal, 8–10 as mild anxiety or depression, 11–14 as moderate anxiety or depression, and 15–21 as severe anxiety or depression.43

Normal healthy volunteers

Twenty four healthy volunteers (16 females; median age 29 years (range 18–55)), recruited by advertisement, were used as control subjects (CS). There was no evidence in any of the subjects of organic or functional gastrointestinal disorder as assessed by detailed clinical history, bowel symptom questionnaire,42 or physical examination. Anxiety and depressive disorders were excluded through the same structured clinical interview (SCID),41 and completion of the HADS.43

Approval for these studies was obtained from the East London and City Health Authority Research Ethics Committee (P01/84), with written informed consent obtained from all patients.

Anorectal physiological investigation

Standard techniques

All subjects underwent detailed standard anorectal physiological investigations, which included station pull through manometry of the anal canal, evaluation of rectal sensory thresholds using a volumetric based balloon distension technique, assessment of pudendal nerve terminal motor latencies, and endoanal ultrasonography.

Manometry was performed using a single channel side hole catheter linked to an Arndorfer-type pneumohydraulic water perfusion system; a pull back technique allowed assessment of functional anal canal length, maximum resting tone, and maximum voluntary squeeze pressures.45 Anal resting tone and squeeze pressures were considered abnormal if they were below 50 cm H2O, which are the lower limits of normal for our unit, as determined from investigation of the 24 control subjects involved in this study plus 32 further controls (56 in total). Rectal sensation was tested by inflating a latex balloon with air at 1 ml/s and determining the threshold volumes for first constant sensation, defecatory desire volume (DDV), and maximum tolerable volume (MTV).46 Patients were considered to have RH if MTV was <100 ml in females or <80 ml in males (determined in 56 healthy control subjects). Pudendal nerve terminal motor latencies (PNTML) were recorded with the St Mark’s pudendal stimulating electrode (Dantec Electronics Ltd, Bristol, UK).47 PNTML are known to increase with age48; patients were considered to have a pudendal neuropathy (either unilateral or bilateral) if PNTMLs exceeded 2.3 ms in those <40 years of age, and exceeded 2.5 ms in those ⩾40 years of age. These values represent the upper limit of normal for our unit. Endoanal ultrasound (10 MHz transducer; B&K Medical, UK) was used to assess sphincter integrity.49

Advanced techniques

Barostat study

An electronic barostat (Synectics Visceral Stimulator; Synectics Medical, Stockholm, Sweden) was used to measure rectal compliance. Employing a stepwise isobaric distension protocol,50 analogue signals from the barostat were amplified and digitised by an interface converter (PC Polygraph HR; Synectics Medical, Enfield, Middlesex, UK) and transmitted to a PC at a sampling rate of 32 Hz for online display and subsequent storage to hard disk. A dedicated software program (Polygram for Windows version 1.1; Synectics Medical, UK) was used for online monitoring and analysis purposes.

With no sedation or bowel preparation, and with subjects lying in the left lateral position, sigmoidoscopy was performed to ensure an empty rectum (all cases). An “infinitely” compliant (that is, within the pressure-volume range studied) barostat bag (maximum capacity 500 ml; Medtronic Functional Diagnostics Zinectics Inc, Utah, USA) mounted on a manometric catheter (internal diameter 3 mm) and fixed at both ends, was then inserted into the rectum, after ensuring that there was no leakage from the system. The catheter was connected to the barostat with an inflation and deflation port. Maximal airflow was 38 ml/s. In an attempt to stabilise basal tone, reduce variability in sensory thresholds and compliance (that is, to improve reproducibility), and familiarise subjects with the procedure, a conditioning distension protocol was performed.51 When inflated, the bag became spherical with a length of 8 cm.

After allowing the system to equilibrate for a further five minutes, bag pressure was then increased from 0 to 32 mm Hg (or maximum toleration) in 2 mm Hg steps and continued for one minute, followed by a one minute rest. At each pressure step, the mean bag volume over the last 30 second segment was recorded.51 Static rectal wall compliance (ml/mm Hg) was calculated as the slope (δV/δP) of the compliance curve between the pressure thresholds of first constant and maximum tolerable distension volumes.50 From 56 control subjects, the normal range for rectal compliance was taken as 8.6–19.1 ml/mm Hg.

Prolonged rectosigmoid manometry

Recording system

Prolonged manometry was carried out using a portable ambulatory recording system (Flexilog 3000; Oakfield Instruments Ltd, Eynsham, Oxon, UK), connected to a Konigsberg solid state manometry catheter (Konigsberg Instruments Inc, Pasadena, California, USA)8 incorporating six transducers, spaced 5 cm apart, and calibrated over a range of 0–200 mm Hg.

No prior bowel preparation or sedation was used. All medications known to affect bowel function, such as loperamide, were discontinued for at least 48 hours prior to the study. A strong suture was tied to the tip of the catheter and grasped by biopsy forceps passed down the channel of a flexible sigmoidoscope. With the patient in the left lateral position, the catheter was then introduced into the rectosigmoid in tandem with the sigmoidoscope, using minimal air insufflation. Under direct vision, the catheter was sited with the most proximal transducer (+25 cm) located in the sigmoid and the most distal transducer (0 cm) in the anal canal. The sigmoidoscope was then carefully withdrawn, ensuring the catheter’s position was maintained. This procedure took no longer than five minutes in all cases. The catheter was then secured in place with tape (Mefix; SCA Molynlycke, UK) and connected to the portable recording system, which was secured in a shoulder harness. Recorded data were stored on a memory card and downloaded to a personal computer for subsequent display and analysis.

Study protocol

In general, prolonged manometric studies were commenced between 13:00 and 15:00. Recordings were started immediately once all the equipment was in place and the patient ambulant. If the catheter was not expelled during defecation, recordings were continued for approximately 20 hours until the next morning when the catheter was removed electively using gentle traction. Fluoroscopic examination in a subgroup of patients (n = 4) confirmed that the position of the catheter was maintained overnight. Subjects were allowed to go home and were encouraged to engage in their normal daily activities. Fluid intake was allowed ad libitum. Neither meal composition nor subject activities was standardised to avoid inducing further stress which may in turn influence colonic motility.52 Instructions were given to retire to bed at 23:00. Subjects were informed to depress an event marker on the recorder when the urge to defecate was perceived. A diary of events recording bedtime, time of wakening, and meal and drink times were carefully recorded.

Data analysis

Overall activity

Automated (quantitative) analysis of overall rectosigmoid contractile activity was initially performed using a validated commercially available computer software program (Flexisoft III, Data Display & Analysis v 2.6.0; Oakfield Instruments Ltd., Oxon, UK). A pressure wave exceeding a threshold of 5 mm Hg, without a simultaneous pressure event occurring in the other four rectal/sigmoid recording channels, was assessed by the computer algorithm as being the consequence of a sigmoid/rectal contraction. These pressure events were predominantly monophasic elevations that had a discernible onset, peak, and offset, and that did not have the features of strain artefact.

For the purposes of analysis, each recording was divided into nocturnal and diurnal periods. The nocturnal period was defined by diary entries. Given the volume of the data recorded, three of the six catheter recording sites were chosen for analysis, at +25 cm, +15 cm, and +10 cm above the mid anal canal, to represent the sigmoid colon, rectosigmoid, and mid-rectum, respectively. As it is very difficult to maintain a point sensor accurately within the high pressure zone of the anal canal,19 marked fluctuations in anal pressure were seen to occur secondary to movement artefact. Consequently, a detailed quantitative analysis of anal motility (that is, from the most distal sensor) was not performed. For rectosigmoid activity, recorded measurements included:

  1. per cent of recording time comprising phasic contractile activity;

  2. contraction frequency;

  3. median contraction amplitude;

  4. maximum contraction amplitude; and

  5. area under the pressure curve.

Specific contractile events

The automated analysis of two specific contractile events, namely high amplitude contractions (HAC) and RMCs, was performed using a separate computer program that has previously been developed and validated “in house” for the computerised assessment of small bowel motility.40,53 Analysis was performed from recording sites at +25 cm, +15 cm, and +10 cm. HACs were defined as individual phasic events which exceeded 50 mm Hg34,52,54 in amplitude. For each subject, the frequency of HACs during the recording period was determined. RMCs were defined as bursts of phasic pressure waves lasting ⩾3 minutes, with a contraction frequency of ⩾2/min.23,30–32 Each RMC was identified visually and demarcated manually as a “region of interest” from which RMC frequency (No/h) could be calculated. Automated analysis of contractions within each region of interest allowed calculation of five further separate variables: (i) periodicity, (ii) complex duration, (iii) contraction frequency, (iv) median contraction amplitude, and (v) area under the pressure curve.

Symptoms

The temporal relationship between the symptom of urgency, as defined by an “event” as recorded by the patient, and rectal/sigmoid motor events (HAC, RMC) was assessed visually. A temporal association was defined as occurring within 60 seconds (30 seconds either side) of depression of the event marker. Qualitative analysis was performed on all recordings by two investigators.

Statistical analysis

Two test groups were defined, based on the results of rectal sensory function testing: those with RH and those with normal sensation (NS). Of the 52 patients with UFI, 27 were found to have RH (22 females; median age 49 years (range 28–72)) and 25 had NS (16 females; median age 45.5 years (range 18–63)).

Clinical, anorectal physiology, and barostat data

Data are expressed as mean (SD) or median (range), depending on whether the recorded values assumed a Gaussian distribution. Results were compared between test groups and CS using one way analysis of variance (ANOVA) or the Kruskal-Wallis test for parametric or non-parametric data, where appropriate, using a commercially available statistical software package (Prism 3.0; GraphPad Software Inc., San Diego, California, USA). For each test, the number of individual subjects in whom recorded values fell outside the respective normal ranges was recorded. Whether contingencies differed between groups was tested using the χ2 or Fisher’s exact test.

Prolonged rectosigmoid manometry

Overall contractile activity and HAC

Data were expressed and results compared in the same way as for clinical, physiological, and barostat data.

RMC variables

Due to the cyclical nature of rectosigmoid motility, prolonged manometric studies produce repeated measures of each RMC variable (periodicity, duration, frequency, amplitude, and area under the curve (AUC)) for each individual subject studied. As a result, conventional statistical methods, which assume a simple independent and non-repeated data structure, should not be employed for such data analysis. Failure to take into account the hierarchical structure in the analysis will give misleading results.55 To account for the non-independence between such repeated measures, we used a mixed effects model for the data analysis, which is a generalisation of an ANOVA model designed to be especially powerful under these circumstances.56 Through application of the mixed effects model, an estimate for the effect of RH or NS patients over CS, its 95% confidence interval (CI), and its statistical significance were calculated.57 In order to maintain normal distribution of residuals (random errors) in the mixed effects model, data for each RMC variable were first transformed logarithmically to derive a single summary statistic for each individual, and the expected effect and its 95% confidence interval from the model were then antilog transformed. Using this methodology, the estimated effect for a variable should be interpreted as relative change. To assess whether the effect for a variable depends on time (day and night), an interaction between group (RH, NS, CS) and time was fitted for each variable. Effect was presented separately according to time if the interaction was significant at the 5% level. The mixed effects model was estimated through an SAS PROC MIXED procedure56 in SAS version 8.2 (SAS Institute, Cary, North Carolina, USA).

RMC frequency, during both nocturnal and diurnal periods, was compared between groups using one way ANOVA. From previous studies,23,24,30–32,39 the upper limit of normal for RMC frequency is reported as 2.0/h during the day and 3.3/h at night. The proportions of individuals in the test groups, with an RMC frequency exceeding the upper limit of normal, were recorded, and contingencies were compared using Fisher’s exact test. The other five RMC variables were evaluated using repeated measures ANOVA by means of the mixed effects model.

Correlations with other variables

Direct examinations of the presence of any correlations between prolonged manometric measurement variables and anorectal physiology and clinical history were made. Linear correlation or regression was used to compare the covariation of two numerical variables. When correlation was applied, parametric (Pearson correlation) or non-parametric (Spearman correlation) methods were used as appropriate.

For all tests, a p value of less than 0.05 (two sided test) was considered to be statistically significant. As this was an exploratory study, no adjustment for multiple comparisons were made in the analysis. Caution must therefore be exercised when a p value is near to 0.05, whereas when a p value is small, the observed difference is unlikely to be spurious.

RESULTS

Clinical history

Duration of symptoms was equivalent between those patients with RH and those with NS (48 months (range 9–432) v 48 months (range 2–216)). In female patients, parity was equivalent between study groups (RH: median 2 (range 0–10); NS: median 2 (range 1–5)).

Reported bowel frequency (fig 1) was significantly higher in patients with RH (median 4.75 bowel actions/24 hours (range 1–8)) than those with NS (median 2 bowel actions/24 hours (range 0.15–9); p = 0.002).

Figure 1

 Reported bowel frequency in the rectal hypersensitivity (RH) and normal rectal sensation (NS) groups. A significantly higher median stool frequency was reported in the RH compared with the NS group (4.75 v 2.0).

Psychological and psychiatric assessment

The structured clinical interview (SCID) for DSM-III-R41 did not reveal the presence of any psychological or psychiatric illnesses in any of the patients or healthy volunteers. The bowel symptom questionnaire and application of the Rome II criteria did not identify any patient or healthy volunteer as having IBS.

The anxiety scale was similar for the RH and NS groups (RH median 7 (range 0–17) v NS 7 (1–14)). Individually, 37% of patients with RH (10/27) were classified as suffering from anxiety compared with 40% in the NS group (10/25). There were also no significant differences in the depression scale between the RH and NS groups (RH median 4 (range 0–9) v NS 4 (0–13)). Again, the proportion of individuals with depression did not differ between the RH and NS groups (11% v 20%). There was no evidence of increased anxiety (median 0 (range 0–4)) or depression (median 1 (range 0–3)) in any of the control subjects.

Anorectal physiology

Anal sphincter function

Twenty one patients with RH (78%; data from one subject missing) and 15 patients with NS (60%; data from one subject missing) had reduced anal squeeze pressures (<50 cm H2O). Only four patients with RH (15%) and four with NS (16%) had both a structurally intact EAS on ultrasound and normal PNTMLs. Pathophysiology of EAS dysfunction was similar between the two groups.

Although there was a significantly greater incidence of internal anal sphincter (IAS) defects identified on endoanal ultrasound in those with RH (46%) than in patients with NS (17%; p = 0.04), IAS function, as defined manometrically, was similar between those with RH and NS. Anal resting tone was reduced (<50 cm H2O) in 58% of patients with RH and 48% of patients with NS.

Rectal sensory function and compliance

By definition, rectal sensory thresholds were significantly lower in the group with RH (mean DDV 48 (SEM 3) ml; MTV 74 (4) ml) than those with NS (DDV 119 (12) ml, p<0.001; MTV 193 (12) ml; p<0.001) and control subjects (DDV 107 (7) ml, p<0.001; MTV 176 (12) ml, p<0.001). There were no differences in rectal sensitivities between patients with NS and CS.

Rectal compliance (fig 2) was reduced (that is, the rectum was “stiffer”) in patients with RH compared with those with NS (mean 10.8 (1.1) ml/mm Hg v 17.8 (1.4) ml/mm Hg; p = 0.001). Patients with NS had elevated rectal compliance in comparison with control subjects (compliance 13.9 (0.4) ml/mm Hg; p<0.05), with three patients having values above the normal range. Although grouped data for rectal compliance were similar between patients with RH and healthy volunteers, 11/27 RH patients (41%) had a compliance value <8.6 ml/mm Hg, which is the lower limit of normality (mean −2SD), as determined previously in our unit. No patient with NS had rectal compliance below that of the normal range (p = 0.0003 v patients with RH).

Figure 2

 Rectal compliance in control subjects (CS) and in those with rectal hypersensitivity (RH) and normal rectal sensation (NS). Compliance of 12 patients with RH and three with NS lay outside the normal range (broken line: 8.6–19.1 ml/mm Hg (mean (2SD)). Rectal compliance was significantly lower in patients with RH compared with NS (p<0.001).

Prolonged rectosigmoid manometry

All subjects tolerated catheter insertion, and no complications were noted during insertion, monitoring, or withdrawal. Although sex matched, control subjects were significantly younger than both RH (p = 0.001) and NS (p = 0.004) patients.

Recording time

Overall recording time in the 76 subjects was 1243 hours (by study group: CS 445 hours; RH 429 hours; NS 369 hours). Mean recording time was 18.6 (0.5) hours in control subjects, which was equivalent to that in patients with RH (15.9 (1.2) hours) but greater than that in patients with NS (15.1 (0.9) hours; p<0.05). Six studies in patients with RH (p<0.03 v control subjects) and five studies in patients with NS (p = 0.05 v control subjects) were terminated prematurely because of catheter expulsion during defecation. In none of these was nocturnal activity recorded. In healthy volunteers, all recording catheters were removed electively. The start time of recordings was similar between groups (RH 13:30 ± 30 minutes; NS 14:30 ± 20 minutes; CS 14:30 ±20 minutes).

Symptoms of urgency

Patients with RH were more frequently symptomatic than either patients with NS or controls. The median number of times per hour the event (symptom) marker was depressed in patients with RH was 0.5 (range 0–4), which was significantly greater that in patients with NS (0.24 (range 0–1.3); p<0.01) and controls (0.05 (range 0–0.3); p<0.001).

Temporal association between event marker and rectal contractile activity

In patients with RH, the event marker was depressed a total of 326 times (median 11 (range 0–57)). Urge to defecate was associated with a HAC in 128 cases (39%), which was significantly higher than in the NS group in whom depression of the event marker correlated with a HAC in 21% of instances (25/117; p = 0.004). In controls, the event marked was depressed in association with a HAC in 28% of instances (9/32), similar to those with NS. Urge to defecate was associated with RMC activity in 21% (67/326), 19% (22/117), and 16% (5/32) in the RH, NS, and CS groups, respectively, with no differences between the groups. Overall, in patients with RH, there was an association between the symptom of urgency and rectosigmoid motor events in 72% of cases compared with 45% and 50% of the NS and CS groups, respectively (p<0.0001 and p<0.03, respectively).

Overall contractile activity (table 1)

Table 1

 Parameters of overall contractile activity at the recording site: +15 cm above the mid anal canal (representing the rectosigmoid) in control subjects (CS) and in those with rectal hypersensitivity (RH) and normal rectal sensation (NS)

The percentage recording time taken up by contractile activity and overall contraction frequency were similar between the groups at all three levels studied. However, median contraction amplitude was significantly greater in patients with RH and CS compared with NS patients at +15 cm (p<0.05 and p<0.05, respectively). Maximum contraction amplitude was also significantly greater in patients with RH and CS compared with NS patients at both +25 cm and +15 cm (+25 cm: p<0.01 and p<0.05, respectively; +15 cm: p<0.01 and p<0.01, respectively). Consequently, the AUC was greater in RH patients than those with NS at all levels (+25 cm: p<0.05; +15 cm: p<0.01; +10 cm: p<0.01). AUC in controls was also greater than in patients with NS at +15 cm (p<0.05). AUC was similar between CS and patients with RH.

HACs

Daytime frequency of HAC (table 2) was increased in patients with RH compared with patients with NS at all levels studied (p<0.01). In addition, nocturnal frequency of HAC was higher in RH patients than in NS patients at both +25 cm (p<0.05) and +10 cm (p<0.05). In comparison with control subjects, the frequency of contractions >50 mm Hg was higher in RH patients at both +25 cm and +15 cm during the day (p<0.05, respectively) and at +15 cm at night (p<0.05).

Table 2

 Frequency of high amplitude contractions (>50 mm Hg/h) during the day and night in control subjects (CS) and in those with rectal hypersensitivity (RH) and normal rectal sensation (NS), at the recording site +15 cm above the mid anal canal

Rectal motor complex (RMC) activity

RMC frequency (fig 3)
Figure 3

 Diurnal (D) and nocturnal (N) rectal motor complex (RMC) frequency in control subjects (CS) and in those with rectal hypersensitivity (RH) and normal rectal sensation (NS), recorded at +15 cm above the mid anal canal. *p<0.05; **p<0.01; ***p<0.001. For data at other recording levels, please refer to the Gut website at http://www.gutjnl.com/supplemental.

For grouped data, RMC frequency in RH patients was significantly increased both during the daytime and at night compared with control subjects at all levels of the study segment (diurnal: +25 cm, p<0.001; +15 cm, p<0.05; +10 cm, p<0.01; nocturnal: p<0.001 at all levels). Likewise, RMC frequency was significantly higher in RH patients than NS patients at all levels during the night (p<0.001) and at +25 cm (p<0.01) and +10 cm (p<0.05) during the day. The frequency of RMCs was similar between NS patients and control subjects both in the diurnal and nocturnal periods. Within groups, RMC frequency was greater at night in both control subjects and patients with NS at +15 cm and +10 cm (+15 cm, p<0.01 and p<0.01, respectively; +10 cm, p<0.001 and p<0.05, respectively). In patients with RH, RMC frequency was similar during the day and at night at all levels.

By site of recording, 15 individual patients with RH (56%) had a diurnal RMC frequency elevated beyond the normal range at +25 cm and +15 cm, and 20 RH patients (74%) had an increased RMC frequency at +10 cm. By comparison, the numbers of patients with NS who had an elevated diurnal RMC frequency were: 2 (8%) at +25 cm (p = 0.0007 v RH), 0 at +15 cm (p<0.0001 v RH), and 3 (12%) at +10 cm (p<0.0001 v RH). Because of defecation (that is, catheter expulsion), it should be noted that of the six RH patients in whom the recording was terminated prematurely, four had increased RMC frequency during the day compared with none of those five patients with NS in whom no nocturnal data were acquired.

At night, the numbers of patients with RH who had an RMC frequency elevated above the normal range was 11 (52%) at +25 cm, 5 (24%) at +15 cm, and 4 (19%) at +10 cm. Only four patients with NS (20%) at +25 cm (p = 0.05 v RH) but none at either +15 cm (p<0.05 v RH) or +10 cm (p = 0.11 v RH) had an abnormally elevated RMC frequency.

RMC variables

Various differences existed between the study groups with regard to four of the five RMC variables—namely periodicity, duration, contraction, frequency, and median contraction amplitude. These results are summarised in tables 3 and 4. For the principal study group (RH), RMC duration was significantly shorter than in controls (mean decrease in duration 13% (95% CI 9–18%); p<0.001), contraction frequency was higher than in CS (mean increase in frequency 14% (5–22%); p<0.01) or NS (mean increase in frequency 14% (3–27%); p<0.05), and median contraction amplitude was lower during the day than in patients with NS (mean decrease in amplitude 9% (1–15%); p<0.05) but higher at night in comparison with controls (mean increase in amplitude 9% (2–15%); p<0.05). For RMC periodicity, results reflected those of RMC frequency (see above). For a full table of all data relating to RMC variables at the three recording levels for the three groups, please refer to the Gut website at http://www.gutjnl.com/supplemental.

Table 3

 Results from the mixed model analysis of individual rectal motor complex parameters in control subjects (CS) and in those with rectal hypersensitivity (RH) and normal rectal sensation (NS)

Table 4

 Results from the mixed model analysis of individual rectal motor complex parameters in control subjects (CS) and in those with rectal hypersensitivity (RH) and normal rectal sensation (NS)

Relationship between RMC variables and clinical history

There was no correlation between RMC frequency and bowel frequency, rectal compliance, or rectal sensation.

DISCUSSION

The findings of this study contribute to our knowledge of the pathophysiology of UFI, providing new information regarding the relationship between rectal sensory and motor function and symptomatology in this condition. We have identified that in a subset of patients with UFI, namely those with RH (as defined by “simple” balloon distension),58 abnormalities of rectosigmoid motor physiology (reduced rectal compliance, increased high amplitude contractions, and altered cyclical contractile activity) exist that are not present in patients with UFI and normal rectal sensation. Such findings may underlie the clinical severity observed in the RH group, including more frequent sensations of urgency and increased bowel frequency, which is in agreement with a recent audit study of over 250 patients with this condition.5

Psychological assessment

It is well documented that increased stress can influence colonic motility,52 and thus an elevated state of anxiety could contribute to the increased reporting of urgency in patients with RH. However, in the current study, there were no differences in the proportions of patients with either RH or NS that suffered from anxiety or depression. RH has been considered to be a marker of IBS58 but based on the Rome II criteria,44 none of the patients in the present study could be diagnosed as having IBS.

Rectal sensation and anal sphincter function

The definition of RH was based on data from healthy subjects whose ages were significantly younger than those of the study population. It has been shown that rectal sensory thresholds may increase with age,59 and therefore there is little risk that patients classified as RH were misclassified in the current study. However, if the control group were older, some patients with NS may have been reclassified as RH. Studies of HAC and RMC activity, stratified by age, are lacking but differences in ages between the control and patient groups may constitute a limitation of this study.

Nearly all patients with UFI were noted to have abnormal EAS function, irrespective of rectal sensory thresholds, supporting the observation that EAS dysfunction is the major pathophysiological factor in this condition.5–7 In contrast, 50% of patients with RH had evidence of IAS disruption, three times higher than in patients with NS. Although the proportions of patients with a functionally poor IAS (as reflected by reduced anal resting tone) were similar between groups, recording solely maximum resting tone does not reflect “global” IAS function. The presence of a weakened unstable IAS may lead to an increased number of internal sphincter relaxations60 and spontaneous anorectal sampling reflexes,61 so producing more frequent sensations of urgency.60 The IAS is a direct continuation of the circular muscle layer of the rectum and thereby provides a possible link between anal and rectal sensorimotor mechanisms. Such a hypothesis remains to be fully explored.

Rectal sensation and compliance

Although not in accordance with guidelines on standardised practice,62 we elected not to administer a rectal enema prior to assessment of compliance on the basis that such action may well disturb the “normal” physiological sensorimotor activity of the rectum, especially in the RH group; indeed, all patients at sigmoidoscopy had essentially empty rectums. As a group, patients with UFI and RH have reduced compliance compared with patients with UFI and NS, with 41% having compliance below the normal range. There was no correlation, however, between compliance and bowel frequency in RH.

That some patients with RH had normal compliance (and normal rectosigmoid contractile activity) suggests that, in a proportion, hypersensitivity may truly involve abnormalities of visceral afferent mechanisms.63 This is supported by the observation that RH patients exhibited enhanced perception of rectal and sigmoid motor events compared with NS and CS, and also from immunohistochemical studies which have suggested the possibility of sensitisation of peripheral mechanisms.64,65

In addition to demonstrating reduced compliance in a proportion of patients with UFI, the finding of compliance greater than normal (that is, hypercompliance) in three patients from the NS group and one patient from the RH group may be a clue to the symptoms of rectal evacuatory difficulty concomitantly reported by some patients with UFI,4,66 and following continence restoring procedures.8,67

Prolonged rectosigmoid manometry

Methods

Prolonged manometric recording of enteric phasic contractile activity is becoming an increasingly recognised clinical tool, especially in the evaluation of upper gastrointestinal function.37,38 In this study, the absence of bowel preparation, and a fully ambulant subject, in whom extrinsic influences were minimised, meant that as close to normal physiological conditions were met. In addition, the use of semi- and fully automated quantitative methods of analysis has been shown to be superior in reliability and reproducibility than manual visual analysis of recordings.53 The computer algorithm overcomes not only problems of variable baseline and sudden changes in pressure due to body movements but also permits objectivity and eliminates observer bias.53 Furthermore, appropriate statistical methodology was employed, taking into account the non-independent and repeated nature of the recorded data. One limitation of prolonged manometry is possible catheter migration. However, maintenance of catheter position was confirmed radiologically in four subjects, and since results were similar at the three levels measured, this issue would not seem of great concern in respect of conclusions which may be drawn.

Symptoms

Not only was the frequency of urgency greater in patients with RH but there was a significantly stronger association between rectosigmoid motor events (notably HACs) and symptoms. This suggests that patients with RH may have enhanced perception of gut stimuli. Hypervigilance to intestinal motor events has also been reported in a proportion of patients with IBS,68,69 in whom RH is a frequent finding.70

Overall activity

The results of this study suggest that the hindgut of patients with RH is hypercontractile compared with those with NS and healthy volunteers. Overall median contraction amplitude, maximum contraction amplitude, and AUC were significantly greater in RH compared with NS, with the frequency of HACs proportionally double in RH compared with NS. High amplitude propagated contractions (because of the short study segment used in the current recordings the term “propagation” was omitted from the description) are the motor correlate of mass intraluminal movement,19,26,54 and the urge to defecate.22,27 It is possible that exaggerated HAC activity may underlie symptomatology (for example, increased bowel frequency, secondary to more rapid colonic transit) in patients with UFI and RH, as has been suggested in patients with IBS.71 It must be stated however that in the present study, recording of pressure activity was confined to the rectosigmoid, and thus abnormal “colonic” motility or transit was not assessed. Assessment of “pancolonic” motor activity might provide more information as to whether the changes observed are confined to the distal hindgut or are more generalised.

A methodological concern regarding manometric recording is that recorded amplitudes are dependent on the luminal diameter of the viscus under study25; the above observations therefore may be artefactual in that a less compliant (as in the RH group) and/or narrower calibre rectum would give higher recorded values than a more compliant greater diameter rectum (or one which contains a significant volume of stool).72 Prolonged recordings of tonic and phasic activity using the barostat may be considered to overcome this limitation.25

RMC activity

The most striking finding of this study was that RMC activity, notably frequency, was significantly higher in RH compared with NS and controls. Such observations should be independent of the methodological concerns described above. Fifty two per cent of patients with RH had a frequency of RMCs exceeding the upper limit of normal.23,30,31 The function and origin of the RMC is still incompletely understood but it has been reported to be triggered by propagating pressure waves from the proximal colon.20,32 It has been further suggested that RMC activity occurs in response to the arrival of stool or gas from the colon. Whether RMCs themselves truly propagate is the subject of debate. RMCs have been observed to move predominantly in a retrograde direction, with less than 5% propagating aborally.32 It is postulated that this would act as a “braking mechanism” to untimely flow of colonic contents32 and so keep the rectum empty.27,31 The increased RMC activity seen in patients with RH may represent a form of protective mechanism to increased flow of colonic contents and/or merely reflect proximal colonic dysmotility. RMCs have also been suggested to be involved in the stimulus to defecate,30 and this may contribute to increased urgency in those RH patients who exhibited overall increased RMC frequency. If the RMC is indeed to be considered a marker of enteric neuromotor function, the abnormalities of various RMC variables demonstrated in this study, using appropriate statistical analyses, suggest a true intrinsic hindgut dysmotility, at least in a proportion of patients with UFI and RH.

In healthy subjects, rectal pressure is thought to increase during a RMC contraction.73 This is invariably accompanied by a rise in anal sphincter tone so that anal canal pressure is always greater than rectal pressure, thus maintaining continence.73 In UFI patients with RH, who have compromised anal sphincter function, the more frequent rises in rectal pressure which may occur as a result of increased RMC activity could afford some explanation as to the greater number of episodes of urgency experienced by these patients.5

Clinical implications

The importance of this study is identification of a distinct pathophysiological subgroup of patients with UFI who exhibit exaggerated symptomatology, which may have important clinical implications for the management of UFI. It is becoming increasingly recognised that advances in the understanding of pathophysiology, rather than taxonomy based on symptoms alone, remain key to improved management.74 In patients with UFI and RH, therapeutic options may include amitriptyline, which has previously been reported to reduce RMC frequency,24 sacral nerve stimulation, which has been shown to reduce urgency,75 and the recently described surgical technique of rectal “augmentation”.8 By contrast, bowel retraining would seem to be less efficacious in the presence of RH.76

In conclusion, this study has attempted to evaluate the relations of rectal sensation and rectal motor activity in patients with UFI through comparison of three homogenous study groups. Only patients with RH have reduced compliance and rectosigmoid dysmotility, as well as enhanced perception of rectal and sigmoid motor events which may be peripherally64 or centrally mediated.77 This sensory and motor disturbance appears to contribute to the symptomatology in such patients. Demonstration of extrasphincteric dysfunction in approximately 50% of all patients with UFI, in whom traditionally solely an anal pathology has been implicated and addressed, cannot be ignored. The association between RH and internal anal sphincter defects warrants further investigation. Finally, this study may have identified a clinical utility for prolonged rectosigmoid manometry in patients with UFI, in whom optimum management may involve more than simple repair of an anatomically disrupted anal sphincter. Further insight may be gained by evaluation of colonic function proximal to the rectosigmoid. Consideration to such comprehensive investigation should be given to patients presenting with UFI, in whom rectal hypersensitivity is demonstrable.

Acknowledgments

CLHC was supported by a Medical Research Council (UK) Clinical Training Fellowship.

REFERENCES

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Footnotes

  • Published online first 24 May 2005

  • Conflict of interest: None declared.

  • This work was presented at the 19th International Symposium on Gastrointestinal Motility, Barcelona, Spain, 5–8 October 2003, and has been previously published in abstract form (

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