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Prion infectivity in variant Creutzfeldt–Jakob disease rectum
  1. J D F Wadsworth,
  2. S Joiner,
  3. K Fox,
  4. J M Linehan,
  5. M Desbruslais,
  6. S Brandner,
  7. E A Asante,
  8. J Collinge
  1. MRC Prion Unit and Department of Neurodegenerative Disease, Institute of Neurology, University College London, National Hospital for Neurology and Neurosurgery, London, UK
  1. Correspondence to:
    Professor J Collinge
    MRC Prion Unit and Department of Neurodegenerative Disease, Institute of Neurology, University College London, National Hospital for Neurology and Neurosurgery, Queen Square, London WC1N 3BG, UK; j.collinge{at}prion.ucl.ac.uk

Abstract

Background: Disease-related prion protein (PrPSc) is readily detectable in lymphoreticular tissues in variant Creutzfeldt–Jakob disease (vCJD), but not in other forms of human prion disease. This distinctive pathogenesis, with the unknown population prevalence of asymptomatic vCJD infection, has led to significant concerns that secondary transmission of vCJD prions will occur through a wide range of surgical procedures. To date PrPSc:prion infectivity ratios have not been determined in vCJD, and it is unknown whether vCJD prions are similar to experimental rodent prions, where PrPSc concentration typically reflects infectious prion titre.

Aim: To investigate prion infectivity in vCJD tissue containing barely detectable levels of PrPSc.

Methods: Transgenic mice expressing only human PrP (Tg(HuPrP129M+/+Prnpo/o)-35 and Tg(HuPrP129M+/+Prnpo/o)-45 mice) were inoculated with brain or rectal tissue from a previously characterised patient with vCJD. These tissues contain the maximum and minimum levels of detectable PrPSc that have been observed in vCJD.

Results: Efficient transmission of prion infection was observed in transgenic mice inoculated with vCJD rectal tissue containing PrPSc at a concentration of 104.7-fold lower than that in vCJD brain.

Conclusions: These data confirm the potential risks for secondary transmission of vCJD prions via gastrointestinal procedures and support the use of PrPSc as a quantitative marker of prion infectivity in vCJD tissues.

  • BSE, bovine spongiform encephalopathy
  • CJD, Creutzfeldt–Jakob disease
  • PBS, phosphate-buffered saline
  • PrP, prion protein
  • PrPSc, disease-related prion protein
  • vCJD, variant Creutzfeldt–Jakob disease

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Footnotes

  • Published Online First 8 June 2006

  • Funding: This work was funded by the UK Medical Research Council and was carried out under the approval from the Institute of Neurology/National Hospital for Neurology and Neurosurgery Local Research Ethics Committee.

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