Article Text

PDF
Exacerbation of murine ileitis by Toll-like receptor 4 mediated sensing of lipopolysaccharide from commensal Escherichia coli
  1. M M Heimesaat1,
  2. A Fischer1,
  3. H-K Jahn1,
  4. J Niebergall1,
  5. M Freudenberg2,
  6. M Blaut3,
  7. O Liesenfeld1,
  8. R R Schumann1,
  9. U B Göbel1,
  10. S Bereswill1
  1. 1Institut für Mikrobiologie und Hygiene, Charité, Universitätsmedizin Berlin, Berlin, Germany
  2. 2Max Planck Institut für Immunbiologie, Freiburg, Germany
  3. 3Deutsches Institut für Ernährungsforschung (DIFE) Potsdam-Rehbrücke, Nuthetal, Germany
  1. Correspondence to:
    S Bereswill
    Institut für Mikrobiologie und Hygiene, Charité, Universitätsmedizin Berlin, Campus Charité Mitte, Dorotheenstr. 96,D-10117 Berlin, Germany; stefan.bereswill{at}charite.de

Abstract

Background: In the course of inflammatory bowel diseases (IBD) and acute murine ileitis following peroral Toxoplasma gondii infection, commensal Escherichia coli accumulate at inflamed mucosal sites and aggravate small intestinal immunopathology.

Aim: To unravel the molecular mechanisms by which commensal E coli exacerbate ileitis.

Methods: Ileitis was investigated in mice that lack Toll-like receptors (TLR) 2 or 4, specific for bacterial lipoproteins (LP) or lipopolysaccharide (LPS), respectively. Gnotobiotic mice, in which any cultivable gut bacteria were eradicated by antibiotic treatment, were used to study the role of LPS in ileitis.

Results: Microbiological analyses revealed that E coli increase in the inflamed ileum. TLR4−/−, but not TLR2−/−, mice displayed reduced mortality and small intestinal immunopathology. Decreased interferon (IFN)-γ and nitric oxide (NO) levels in the inflamed terminal ileum of TLR4−/− mice indicated that TLR4 signalling aggravates ileitis via local mediator release from immune cells. E coli strains isolated from the inflamed ileum activated cultured mouse macrophages and induced TLR4-dependent nuclear factor κB activation and NO production in human embryonic kidney 293 cells and in peritoneal macrophages, respectively. Most strikingly, in contrast with wild-type mice, gnotobiotic TLR4−/− mice were protected from induction of ileitis by treatment with purified E coli lipid A or colonisation with live E coli. Finally, prophylactic treatment with the LPS scavenger polymyxin B ameliorated T gondii-induced ileitis.

Conclusion: These findings highlight the innate immune system as a key player in T gondii-induced ileal immunopathology. Treatment with LPS or TLR4 antagonists may represent a novel strategy for prophylaxis and/or therapy of small intestinal inflammation in IBD.

  • CD, Crohn’s disease
  • cfu, colony-forming units
  • DGGE, denaturing gradient gel electrophoresis
  • HEK, human embryonic kidney cells
  • IBD, inflammatory bowel disease
  • LP, lipoprotein
  • LPS, lipopolysaccharide
  • IFN-γ, interferon gamma
  • MLN, mesenteric lymph nodes
  • NFκB, nuclear factor κB
  • NO, nitric oxide
  • pi, postinfection
  • RPMI, Rosewell Park Memorial Institute
  • PBS, phosphate-buffered saline
  • PM, peritoneal macrophage
  • SNP, single-nucleotide polymorphisms
  • TLR, Toll-like receptor
View Full Text

Statistics from Altmetric.com

Footnotes

  • Published Online First 25 January 2007

  • Funding: This work was supported by grants from the Deutsche Forschungsgemeinschaft to UBG, OL, RRS (KFO104/project 6; SFB633/projects A7, B6) and MF (DFG-SPP1110/project Fr448/4-3).

  • Competing interests: None.

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

Linked Articles

  • Digest
    BMJ Publishing Group Ltd and British Society of Gastroenterology