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Potentiation of TRPV4 signalling by histamine and serotonin: an important mechanism for visceral hypersensitivity
  1. Nicolas Cenac1,2,
  2. Christophe Altier3,
  3. Jean-Paul Motta1,2,
  4. Emilie d'Aldebert1,2,
  5. Sophie Galeano1,2,
  6. Gerald W Zamponi3,
  7. Nathalie Vergnolle1,2,3
  1. 1INSERM U563, Centre de Physiopathologie de Toulouse Purpan, Toulouse, France
  2. 2Université Toulouse III Paul Sabatier, Toulouse, France
  3. 3Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta, Canada
  1. Correspondence to Dr Nathalie Vergnolle, INSERM U 563, CHU Purpan, BP 3028, 31024 Toulouse Cedex, France; nathalie.vergnolle{at}inserm.fr

Abstract

Background Although evidence points to a role for histamine and serotonin in visceral hypersensitivity, activation of calcium channels such as transient receptor potential vanilloid 4 (TRPV4) also causes visceral hypersensitivity. We hypothesised that TRPV4 is important for the generation of hypersensitivity, mediating histamine- and serotonin-induced visceral hypersensitivity.

Methods In response to histamine, serotonin and/or TRPV4 agonist (4αPDD), calcium signals and TRPV4 localisation studies were performed on dorsal root ganglia (DRG) neurons projecting from the colon. To evaluate visceral nociception, colorectal distension (CRD) was performed in mice treated with serotonin or histamine and with 4αPDD. Intrathecal injection of TRPV4 silencer RNA (SiRNA) or mismatch SiRNA was used to target TRPV4 expression.

Results Pre-exposure of DRG neurons projecting from the colon, to histamine or serotonin, increased Ca2+ responses induced by 4αPDD by a protein kinase C (PKC), phospholipase Cβ (PLCβ), mitogen-activated protein kinase kinase (MAPKK) and phospholipase A2 (PLA2)-dependent mechanisms. Serotonin or histamine treatments enhanced TRPV4 expression at the plasma membrane by a MAPKK mechanism. Hypersensitivity induced by serotonin or histamine were both significantly inhibited by TRPV4 SiRNA intrathecal injection. Administration of sub-nociceptive doses of serotonin or histamine potentiated 4αPDD-induced hypersensitivity in response to CRD.

Conclusions Serotonin and histamine sensitise TRPV4 response to 4αPDD both in vivo (increased visceral hypersensitivity) and in vitro, in sensory neurons, by a PKC, PLA2, PLCβ and MAPKK-dependent mechanism. Serotonin and histamine caused a MAPKK-dependent increase in TRPV4 expression in colonic sensory neurons plasma membranes. Further, histamine- or serotonin-mediated visceral hypersensitivity depend on TRPV4 expression in sensory neurons. TRPV4 appears as a common mechanism to several known mediators of visceral hypersensitivity.

  • Visceral pain
  • transient receptor potential vanilloid
  • histamine
  • serotonin
  • sensory neurons
  • summary box
  • abdominal pain
  • ION channels
  • neurogastroenterology
  • serotonin

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Footnotes

  • NC and CA contributed equally to this work.

  • Funding This research was supported by grants from the Canadian Institute of Health Research (to GWZ and NV), the Crohn's and Colitis Foundation (to NV), the Fondation Bettencourt-Schueller (to NV), the INSERM-Avenir program (to NV), and the Fondation Schlumberger (to NV).

  • Competing interests None.

  • Ethics approval All procedures were approved by institutional Animal Care Committees and received an accreditation number (veterinary services).

  • Provenance and peer review Not commissioned; externally peer reviewed.

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