Objectives Abdominal pain is a serious cause of morbidity in patients with inflammatory bowel disease. To better understand the mechanisms and potentially identify new targets for treatment, the effects of inflammatory supernatant from colonic biopsies of patients with active ulcerative colitis (UC) on mouse colonic nociceptive dorsal root ganglia neurons were examined.
Methods Acutely dissociated dorsal root ganglia neurons innervating the mouse colon were incubated in supernatants obtained from colonic biopsies from patients with UC. Whole-cell patch clamp recordings were obtained to examine the effects on neuronal excitability. The role of tumour necrosis factor α (TNFα) was studied using TNFα receptor (TNFR) knockout mice and comparing supernatant and TNFα actions.
Results UC supernatants significantly decreased the rheobase and increased action potential discharge, indicating increased neuronal excitability. Human biopsies exhibited high levels of TNFα, and mouse colonic neurons only exhibited TNFR1 mRNA. Incubation with TNFα recapitulated the supernatant effects on neuronal excitability, and supernatant and TNFα actions were almost completely blocked in TNFR knockout mice. In voltage clamp studies, transient IA and IK currents were suppressed and Nav 1.8 currents were enhanced by TNFα and UC supernatant, suggesting that multiple underlying mechanisms contributed to the enhanced excitability.
Conclusions UC supernatants enhance neuronal excitability of sensory dorsal root ganglia neurons innervating the colon. TNFα is a key mediator which acts at neuronal TNFR1 to modulate Kv and Nav currents. Together these data provide a number of potential new targets for pain management in UC.
- Abdominal pain
- inflammatory bowel disease TNFα
- ulcerative colitis
Statistics from Altmetric.com
Funding Crohn's and Colitis Foundation of Canada, and Canadian Institutes of Health Research.
Competing interests None.
Ethics approval This study was conducted with the approval of the Queen's University Human Ethics Committee.
Provenance and peer review Not commissioned; externally peer reviewed.
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.