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OC-041 Changes in the expression of cellular iron transport machinery occur in pre-neoplastic lesions within the colon in both sporadic and familial polyposis
  1. M J Brookes1,
  2. S Ford2,
  3. G Caldwell2,
  4. C Jones2,
  5. T Iqbal2,
  6. C Tselepis2
  1. 1Department of Gastroenterology, New Cross Hospital, Wolverhampton, UK
  2. 2Cancer Studies, University of Birmingham, Birmingham, UK

Abstract

Introduction Sporadic colorectal adenocarcinoma (CRC) develops through the adenoma-carcinoma sequence involving in the tumour suppressor gene adenomatous polyposis coli (APC). Familial adenomatous polyposis coli (FAP) is a condition in which germline mutations in APC lead to early adenoma and tumour formation. Mutations in APC lead to increased Wnt signalling, the main oncogenic pathway in colon cancer, upregulating c-myc.

Increased expression of the cellular iron import proteins (TfR1 and DMT-1) are found in CRC, and lead to increased intracellular iron. The aim of this study is to determine whether these changes can be found in pre-cancerous adenomatous polyps in sporadic and familial adenomatous polyposis.

Methods Colonic tissue from i) sporadic adenomas (n=15) and ii) familial adenomatous polyposis coli (n=10) was collected with matched normal colon. Real-time PCR was used to determine the expression of the iron transport proteins (TFR1, DMT1, ferroportin and ferritin) and c-myc.

Results Sporadic and familial adenomatous tissue showed a significant fold increase in TfR1, DMT1 and c-myc expression in 15/25, 16/25 and 14/25 tissue samples, respectively. Furthermore both TfR1 and DMT1 were tightly correlated with c-myc expression (R2>0.94 and 0.81, respectively).

Conclusion This study demonstrates that changes in the expression of iron transport machinery occur in the pre-cancerous adenomatous polyps in both sporadic and familial polyposis and may be regulated by the oncogene c-myc.

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