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Human visceral afferent recordings: preliminary report
  1. Madusha Peiris1,
  2. David C Bulmer1,
  3. Mark D Baker1,
  4. George Boundouki1,
  5. Sidhartha Sinha1,
  6. Anthony Hobson1,
  7. Kevin Lee2,
  8. Qasim Aziz1,
  9. Charles H Knowles1
  1. 1Neurogastroenterology Group, Centre for Digestive Diseases, Blizard Institute of Cellular and Molecular Science, Barts and the London School of Medicine and Dentistry, Queen Mary University London, London, UK
  2. 2Epinova DPU, Immuno-Inflammation CEDD, GlaxoSmithKline, Stevenage, Hertfordshire, UK
  1. Correspondence to Mr Charles H Knowles, Centre for Academic Surgery, 3rd Floor Alexandra Wing, Royal London Hospital, London E1 1BB, UK; c.h.knowles{at}qmul.ac.uk

Abstract

Background Conditions characterised by chronic visceral pain represent a significant healthcare burden with limited treatment options. While animal models have provided insights into potential mechanisms of visceral nociception and identified candidate drug targets, these have not translated into successful treatments in humans.

Objective To develop an in vitro afferent nerve preparation using surgically excised freshly isolated human colon and vermiform appendix-mesentery tissues.

Methods Non-inflamed appendix (n=18) and colon (n=9) were collected from patients undergoing right and left hemicolectomy. Electrophysiological recordings were made from mesenteric nerves and the tissue stimulated chemically and mechanically.

Results Ongoing neuronal activity was sparse and where units occurred peak firing rates were: colon (2.0±0.4 spikes/s, n=4) and appendix (2.4±0.6 spikes/s, n=9). Afferent nerves innervating the appendix responded with a significant increase in activity following stimulation with inflammatory mediators (73±10.6 vs 3.0±0.3 spikes/s, n=6, p<0.001, inflammatory mediator vs baseline) and capsaicin (63±15.8 vs 2±0.3 spikes/s, n=3, p<0.001, capsaicin vs buffer). Afferent nerves innervating the colon responded with increased activity to blunt probing of the serosal surface.

Conclusions This first-in-human study demonstrates afferent nerve recordings from human gut tissue ex vivo and shows that tissue may be stimulated both chemically and mechanically to study neuronal responses. Collectively, the results provide preliminary evidence to validate this in vitro human tissue model as one that may aid future disease mechanistic studies and candidate drug testing.

  • Visceral pain
  • human
  • afferent
  • electrophysiology
  • electrophysiology
  • nerve - gut interactions
  • visceral nociception

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Footnotes

  • See Commentary, p 151

  • MP and DB are joint first authors on this paper.

  • Funding This work was supported by CORE Digestive Diseases (CHK, QA and MP) and The Royal College of Surgeons (SS).

  • Competing interests None.

  • Ethics approval This study was conducted with the approval of the East London and The City HA Local Research Ethics Committee (NREC 09/H0704/2).

  • Provenance and peer review Not commissioned; externally peer reviewed.

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