Article Text


P60 Pre-treatment IP-10 concentrations are associated with a sustained virological response in HIV/HCV co-infected patients
  1. D Joshi,
  2. M Bruce,
  3. A Suddle,
  4. C Taylor,
  5. I Carey,
  6. K Agarwal
  1. King's College Hospital


Introduction Treatment of chronic HCV (cHCV) in HIV positive patients with pegylated interferon (PEG-IFN) and ribavirin (RIB) is associated with poorer treatment outcomes and an increased side effect profile. Strong immune T helper type 1 responses towards HCV determine outcome of infection. Interferon γ inducible protein 10 (IP-10) has been shown to correlate with treatment response in cHCV mono-infection but limited data are available for HIV co-infected patients.

Aim To investigate whether pre-treatment plasma levels of cytokines/chemokines differ between HIV/HCV co-infected and HCV mono-infected patients and whether they can predict response to PEG-IFN and RIB therapy.

Method Pre-treatment plasma samples were studied in HIV positive patients co-infected with cHCV and HIV negative cHCV patients. Plasma levels of IFN-γ, interleukin (IL)-2, IL-4, IL-6, IL-8, IL-10 and IP-10 (all pg/ml) were measured by ELISA. Patients were matched according to genotype, fibrosis stage and age. All patients were treated with PEG-IFN α 2a and weight based RIB for 24 weeks or 48 weeks according to genotype. All patients were previously treatment naive. Virological response was divided into SVR, non-response (NR) and responder relapse (RR). All results are presented as medians.

Results 37 HIV positive patients (CD4 count 495 cells/ml, undetectable HIV viral load in 86%) co-infected with HCV ([HCV viral load (VL) 1.68E6 IU/ml, 54% genotype 1, 7 (19%) patients with advanced fibrosis on biopsy (Ishak F>5)] were divided according virological response [n=23 SVR (62%), n=7 RR, n=7 NR]. 35 HIV negative patients with cHCV [HCV VL 2.12E6, 64% genotype 1, 5 (14%) patients with advanced fibrosis (Ishak F>5) were also divided according to virological response [n=23 SVR (66%), n=7 RR, n=6 NR]. Irrespective of HIV status, IP-10 (62 vs 105, p=0.006) and IL-6 (4 vs 7, p=0.03) levels were lower at baseline in patients who achieved an SVR. Baseline IP-10 (92 vs 49, p=0.009) and IL-8 (39 vs 22, p=0.01) were higher in the HIV positive group but the concentrations of IFN-γ, IL-2, IL-4, IL-6, IL-10 were similar between the two groups. In HIV positive patients baseline IP-10 concentrations were lower in those who achieved a SVR (84 vs 107 p=0.02) and also differed according to treatment response (SVR, 84 vs RR, 124 vs NR, 107 p=0.03).

Conclusion In our cohort IP-10 acted as a robust predictor of SVR among HIV/HCV co-infected and HCV mono-infected patients. IP-10 could serve as a pre-treatment biomarker to help identify patients who will achieve a SVR.

Statistics from

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.