Gut-derived lipopolysaccharide augments adipose macrophage accumulation but is not essential for impaired glucose or insulin tolerance in mice
- Robert Caesar1,2,
- Christopher S Reigstad1,2,
- Helene Kling Bäckhed1,2,
- Christoph Reinhardt1,2,
- Maria Ketonen1,2,
- Gunnel Östergren Lundén1,2,
- Patrice D Cani3,
- Fredrik Bäckhed1,2
- 1Sahlgrenska Center for Cardiovascular and Metabolic Research/Wallenberg Laboratory, Gothenburg, Sweden
- 2Department of Molecular and Clinical Medicine, University of Gothenburg, Gothenburg, Sweden
- 3Université catholique de Louvain, Louvain Drug Research Institute, Metabolism and Nutrition Research Group, Brussels, Belgium
- Correspondence to Dr Fredrik Bäckhed, Wallenberg Laboratory, Sahlgrenska University Hospital, S-413 45 Gothenburg, Sweden;
Contributors RC: contributed to the experimental plan, performed research, analysed data, wrote the manuscript; CSR: contributed to the experimental plan, performed research, analysed data, commented on the manuscript; HKB, CR, PDC: performed research, analysed data, commented on the manuscript; MK, GÖL: performed research, analysed data; FB: conceived the overall research plan, analysed data, wrote the manuscript.
- Revised 27 February 2012
- Accepted 12 March 2012
- Published Online First 25 April 2012
Background Obesity is associated with accumulation of macrophages in white adipose tissue (WAT), which contribute to the development of insulin resistance. Germ-free (GF) mice have reduced adiposity and are protected against diet-induced obesity,
Objective To investigate whether the gut microbiota and, specifically, gut-derived lipopolysaccharide (LPS) promote WAT inflammation and contribute to impaired glucose metabolism.
Method Macrophage composition and expression of proinflammatory and anti-inflammatory markers were compared in WAT of GF, conventionally raised and Escherichia coli-monocolonised mice. Additionally, glucose and insulin tolerance in these mice was determined.
Results The presence of a gut microbiota resulted in impaired glucose metabolism and increased macrophage accumulation and polarisation towards the proinflammatory M1 phenotype in WAT. Monocolonisation of GF mice for 4 weeks with E.coli W3110 or the isogenic strain MLK1067 (which expresses LPS with reduced immunogenicity) resulted in impaired glucose and insulin tolerance and promoted M1 polarisation of CD11b cells in WAT. However, colonisation with E.coli W3110 but not MLK1067 promoted macrophage accumulation and upregulation of proinflammatory and anti-inflammatory gene expression as well as JNK phosphorylation.
Conclusion Gut microbiota induced LPS-dependent macrophage accumulation in WAT, whereas impairment of systemic glucose metabolism was not dependent on LPS. These results indicate that macrophage accumulation in WAT does not always correlate with impaired glucose metabolism.
- gnotobiotic mice
- gut microbiota
- metabolic inflammation, inflammation
- E coli
- energy metabolism
- inflammatory diseases
- macrophages, obesity
- colonic microflora
- intestinal permeability
- mucosal immunity
- cardiovascular disease
- intestinal epithelium
- intestinal tract
- lipid metabolism
Funding This work was supported by the Swedish Research Council, Swedish Foundation for Strategic Research, Swedish Diabetes Foundation, Petrus and Augusta Hedlund Foundation, Torsten and Ragnar Söderbergs Foundations, Novo Nordisk Foundation, TORNADO (FP7-KBBE-222720, http://www.fp7tornado.eu/), AstraZeneca through collaboration with the Sahlgrenska Academy, and a LUA-ALF grant from Västra Götalandsregionen. CSR is the recipient of a postdoctoral fellowship from the Wenner-Gren foundation. PDC is a research associate from the FRS-FNRS (Fonds de la recherche scientifique, Belgium) and is the recipient of grants from the FSR (Fonds Spéciaux de Recherche, UCL) and the FRSM (Fonds de la Recherche Scientifique Médicale).
Competing interests None.
Provenance and peer review Not commissioned; externally peer reviewed.
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