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PWE-150 “The Early Growth Response Genes Induce Apoptosis in Colon Cancer Cells”
  1. A Koulouris1
  1. 1Gastroenterology-Experimental Immunology, Queen Mary, University of London, London, UK

Abstract

Introduction The Egr familly (Early Growth Respone Genes) of zinc finger transcription factors, which consists of four members; Egr-1, –2, –3 and –4, have been proved to have dynamic functions in the regulation of cell growth and the immune responses.Moreover, in a number of malignancies-which is a cell growth and immune related process- it seems that Egr1 and 2 induce apoptosis leading to the inhibition of the tumour growth. The present study was designed to answer the question whether colon cancer cells undergo apoptosis when the EGR genes are exogenously introduced and if the presence of a mutant p53 can affect this apoptotic pathway.

Methods Two cell lines deriving from human colon cancer; one p53 negative (DLD1) and another p53 positive (HCT116) were transfected with Egr-1, –2 and –3 and a fluorescent protein which was the marker of the transfection. The transfected cells were incubated for 48 hours. Flow cytometry was used to create a pure population of transfected cells and 24hours later these cells were examined in the fluoroscopic microscope and compared with the controls.

Results We found that all three Egr members can suppress tumour cell growth suggesting that the function of Egr in the control of cell growth is not associated with the function of p53. In addition to the growth arrest, the transfected cells changed morphology to round shape indicating of senescence.

Conclusion This may suggest that Egr molecules are important to control the unwanted growth in response to malignant transformation. Our results not only demonstrated an important function of Egr molecules and also indicate the therapeutic potential for the treatment of tumour.

Disclosure of Interest None Declared.

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