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Original article
Effects of HDV infection and pegylated interferon α treatment on the natural killer cell compartment in chronically infected individuals
  1. Sebastian Lunemann1,
  2. David F G Malone2,
  3. Jan Grabowski1,
  4. Kerstin Port1,
  5. Vivien Béziat2,
  6. Birgit Bremer1,
  7. Karl-Johan Malmberg2,3,4,
  8. Michael P Manns1,
  9. Johan K Sandberg2,
  10. Markus Cornberg1,
  11. Hans-Gustaf Ljunggren2,
  12. Heiner Wedemeyer1,
  13. Niklas K Björkström2,5
  1. 1Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany
  2. 2Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden
  3. 3Institute for Cancer Research, Oslo University Hospital, Oslo, Norway
  4. 4Institute for Cancer Research, Institute of Clinical Medicine, University of Oslo, Oslo, Norway
  5. 5Liver Immunology Laboratory, Department of Medicine, Karolinska Institutet, Stockholm, Sweden
  1. Correspondence to Dr Niklas K Björkström, Center for Infectious Medicine, F59, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm S-14186, Sweden; niklas.bjorkstrom{at}ki.se

Abstract

Objective Although hepatitis delta is considered an immune-mediated disease, adaptive immune responses to hepatitis delta virus (HDV) are hardly detectable. Thus, the role of other immune responses, including those mediated by natural killer (NK) cells, must be considered in HDV pathogenesis and in treatments with immune-stimulating agents such as interferon (IFN)α. However, the phenotype and function of NK cells in chronic HDV infection, or in HDV-infected individuals undergoing IFNα treatment, have not been extensively studied.

Design We performed an extensive analysis of NK cells in chronically HDV-infected patients before and during treatment with IFNα, and compared the results with those for patients with HBV mono-infection as well as healthy controls.

Results In untreated HDV-infected patients, a higher than normal frequency of NK cells was observed in peripheral blood with unaltered phenotypic NK cell differentiation status. In contrast, long-term IFNα treatment of HDV-infected patients caused a significant change in NK cell differentiation status, with selective loss of terminally differentiated NK cells and, in parallel, a relative enrichment in immature NK cell subsets. Treatment was associated with marked functional impairment of the NK cells, which was independent of the changes in NK cell differentiation status. Furthermore, treatment polarised NK cell IFN signalling from STAT4 towards STAT1 dependency. Strikingly, a high frequency of CD56dim NK cells at baseline was positively associated with IFNα treatment outcome in the patients.

Conclusions We describe in detail how HDV infection, and IFNα treatment of this infection, affects the NK cell compartment and what consequences this has for the functional capacity of NK cells.

  • Hepatitis D
  • Interferon-Alpha
  • Immunology

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