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Adiponectin plays a protective role in caerulein-induced acute pancreatitis in high-fat diet-fed mice
  1. Hiroshi Araki (araki{at}gh.med.osaka-u.ac.jp)
  1. Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Japan
    1. Tamao Nishihara (tamaon{at}gh.med.osaka-u.ac.jp)
    1. Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Japan
      1. Morihiro Matsuda
      1. Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Japan
        1. Atsunori Fukuhara
        1. Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Japan
          1. Shinji Kihara
          1. Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Japan
            1. Tohru Funahashi
            1. Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Japan
              1. Tatsuki R Kataoka
              1. Department of Stem Cell Pathology, Graduate School of Medicine, Osaka University, Japan
                1. Yoshihiro Kamada
                1. Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Japan
                  1. Tatsuya Kiyohara
                  1. Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Japan
                    1. Shinji Tamura
                    1. Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Japan
                      1. Norio Hayashi
                      1. Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Japan
                        1. Iichiro Shimomura
                        1. Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Japan

                          Abstract

                          Background: Obesity is a risk factor for acute pancreatitis (AP), but the molecular mechanism remains unclear. Adiponectin, an adipose tissue-derived secretory factor, has anti-inflammatory properties in addition to various biological functions, and its plasma concentrations are reduced in obese subjects. However, the role of adiponectin in AP has not been investigated.

                          Aim: To determine the effects of adiponectin on AP.

                          Methods: We investigated the effects of adiponectin on experimental AP by using adiponectin-knockout (APN-KO) mice and adenovirus-mediated adiponectin overexpression. AP was induced by 10 hourly intraperitoneal injections of low-dose caerulein (10 μg/kg) after 2-week feeding of normal chow or high-fat diet (HFD) in wild-type (WT) and APN-KO mice. We evaluated the severity of AP biochemically and morphologically.

                          Results: Low-dose caerulein treatment did not induce pancreatic damage, in both WT and APN-KO mice under normal chow feeding. APN-KO mice, but not WT mice, fed HFD then treated with caerulein developed pancreatic damage and inflammation, accompanied by increased macrophage/neutrophil infiltration and upregulation of pro-inflammatory mediators such as tumor necrosis factor-α in the pancreas. Adenovirus-mediated overexpression of adiponectin attenuated the severity of HFD/caerulein-induced AP in APN-KO mice.

                          Conclusions: Adiponectin plays a protective role in caerulein-induced AP in HFD-fed mice.

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