Article Text

other Versions

PDF
Adiponectin Suppresses Colorectal Carcinogenesis under the High-fat Diet Condition
  1. Toshio Fujisawa (tossshy{at}ybb.ne.jp)
  1. Yokohama City University School of Medicine, Japan
    1. Hiroki Endo (t066011b{at}yokohama-cu.ac.jp)
    1. Yokohama City University School of Medicine, Japan
      1. Ayako Tomimoto (ayatomi{at}hotmail.co.jp)
      1. Yokohama City University School of Medicine, Japan
        1. Michiko Sugiyama (t076038e{at}yokohama-cu.ac.jp)
        1. Yokohama City University School of Medicine, Japan
          1. Hirokazu Takahashi (hirokazu{at}med.yokohama-cu.ac.jp)
          1. Yokohama City University School of Medicine, Japan
            1. Satoru Saito (ssai1423{at}yokohama-cu.ac.jp)
            1. Yokohama City University School of Medicine, Japan
              1. Masahiko Inamori (inamorim{at}med.yokohama-cu.ac.jp)
              1. Yokohama City University School of Medicine, Japan
                1. Noriko Nakajima (tenko{at}nih.go.jp)
                1. National Institute of Infectious Diseases, Japan
                  1. Masatoshi Watanabe (jes76{at}med.yokohama-cu.ac.jp)
                  1. Graduate School of Engineering, Yokohama National University, Japan
                    1. Naoto Kubota (hiraga{at}yokohama-cu.ac.jp)
                    1. Graduate School of Medicine, University of Tokyo, Japan
                      1. Toshimasa Yamauchi (chiduccyo{at}yahoo.co.jp)
                      1. Graduate School of Medicine, University of Tokyo, Japan
                        1. Takashi Kadowaki (yokohamaikyoku{at}yahoo.co.jp)
                        1. Graduate School of Medicine, University of Tokyo, Japan
                          1. Koichiro Wada (kwada{at}dent.osaka-u.ac.jp)
                          1. Graduate School of Dentistry, Osaka University, Japan
                            1. Hitoshi Nakagama (hnakagam{at}gan2.res.ncc.go.jp)
                            1. National Cancer Center Research Institute, Japan
                              1. Atsushi Nakajima (nakajima-tky{at}umin.ac.jp)
                              1. Yokohama City University School of Medicine, Japan

                                Abstract

                                Background & Aims: The effect of adiponectin on colorectal carcinogenesis has been proposed but not fully investigated. We investigated the effect of adiponectin deficiency on the development of colorectal cancer.

                                Methods: We generated three types of gene-deficient mice; adiponectin-deficient, Adiponectin receptor 1-deficient and Adiponectin receptor 2-deficient, and investigated chemical-induced colon polyp formation and cell proliferation in colon epithelium. Western blot analysis was performed to elucidate the mechanism which affected colorectal carcinogenesis by adiponectin deficiency.

                                Results: The numbers of colon polyps were significantly increased in adiponectin-deficient mice compared with wild-type mice fed with high-fat diet. However, no difference was observed between wild-type and adiponectin-deficient mice fed with basal diet. A significant increase in cell proliferative activity was also observed in the colonic epithelium of the adiponectin-deficient mice when compared with wild-type mice fed with high-fat diet, however, no difference was observed between wild-type and adiponectin-deficient mice fed with basal diet. Similarly, an increase in epithelial cell proliferation was observed in Adiponectin receptor 1-deficient mice, but not in Adiponectin receptor 2-deficient mice. Western blot analysis revealed activation of mammalian target of rapamycin, p70 S6 kinase, S6 protein and inactivation of AMP-activated protein kinase in the colon epithelium of adiponectin-deficient mice fed with high-fat diet.

                                Conclusions: Adiponectin suppresses colonic epithelial proliferation via inhibition of mammalian target of rapamycin pathway under the high-fat diet, but not under basal diet. These studies indicate a novel mechanism of suppression of colorectal carcinogenesis induced by western-style high-fat diet.

                                Statistics from Altmetric.com

                                Request permissions

                                If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

                                Linked Articles

                                • Digest
                                  Robin Spiller Magnus Simren