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Probiotic and postbiotic activity in health and disease: comparison on a novel polarised ex-vivo organ culture model
  1. Katerina Tsilingiri1,
  2. Theolis Barbosa1,
  3. Giuseppe Penna1,
  4. Flavio Caprioli2,
  5. Angelica Sonzogni3,
  6. Giuseppe Viale3,
  7. Maria Rescigno1
  1. 1Department of Experimental Oncology, European Institute of Oncology, Milan, Italy
  2. 2U.O. Gastroenterologia 2, Fondazione IRCCS Ca' Granda Ospedale Policlinico di Milano, Milan, Italy
  3. 3Department of Pathology and Laboratory Medicine, European Institute of Oncology, Milan, Italy
  1. Correspondence to Dr Maria Rescigno, Department of Experimental Oncology, European Institute of Oncology, Via Adamello 16, 20141 Milan, Italy; maria.rescigno{at}ifom-ieo-campus.it

Abstract

Background and aims Probiotics and their metabolic products, here called postbiotics, have been proposed as food supplements for a healthier intestinal homeostasis, but also as therapeutic aids in inflammatory bowel disease (IBD) with, however, very little clinical benefit. This may be due to the lack of reliable preclinical models for testing the efficacy of different strains.

Methods The activity of three probiotic strains of Lactobacillus (or a postbiotic) was analysed and compared with a pathogenic strain of Salmonella on a novel organ culture system of human healthy and IBD intestinal mucosa developed in our laboratory. The system maintains an apical to basolateral polarity during stimulation due to the presence of a glued cave cylinder. The cylinder is detached at the end of the experiment and the tissue is processed for histology and immunohistochemistry. Cytokines released from the basolateral side are analysed.

Results The model system provides several physiological characteristics typical of a mucosal microenvironment including the presence of an organised mucus layer and an apical to basolateral polarity. Polarised administration of bacteria is critical to control the ensuing immune response as it mimics the physiological entrance of bacteria. The authors show that probiotics are not always beneficial for the healthy host and can also be detrimental in inflamed IBD. This study shows that a potent postbiotic can protect against the inflammatory properties of invasive Salmonella on healthy tissue and also downregulate ongoing inflammatory processes in IBD tissue.

Conclusions Probiotics can have inflammatory activities in both healthy and IBD tissue. Valid preclinical data on proper model systems should therefore be obtained before specific probiotic strains enter the clinics, especially if administered during acute inflammatory responses. Postbiotics may be a safe alternative for the treatment of patients with IBD in the acute inflammatory phase.

  • Immunology
  • immunoregulation
  • immunotherapy
  • infectious disease
  • intestinal bacteria
  • Crohn's disease
  • ulcerative colitis
  • gut immunology
  • gut inflammation
  • immune response
  • anti-bacterial mucosal immunity
  • antigen presentation
  • epithelial barrier

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Footnotes

  • Funding This work was supported by grants from the 7th EU framework program (IBDase, ERC: Dendroworld) and Fondazione Cariplo to MR and support by a Marie Curie international training mobility network (Cross-Talk, grant agreement No: 21553-2) to KT.

  • Competing interests None.

  • Ethics approval Only material that was not required for diagnosis was used and all patients signed an informed consent approved by the IEO Institutional Review Board which allows researchers to use exceeding material for research purposes.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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