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Original article
Age-dependent shift in macrophage polarisation causes inflammation-mediated degeneration of enteric nervous system
  1. Laren Becker1,
  2. Linh Nguyen1,
  3. Jaspreet Gill1,
  4. Subhash Kulkarni2,
  5. Pankaj Jay Pasricha2,
  6. Aida Habtezion1
  1. 1Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University, Stanford, California, USA
  2. 2Division of Gastroenterology and Hepatology, Department of Medicine, Johns Hopkins University, Baltimore, Maryland, USA
  1. Correspondence to Dr Laren Becker, Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA; lsbecker{at}stanford.edu

Abstract

Objective The enteric nervous system (ENS) undergoes neuronal loss and degenerative changes with age. The cause of this neurodegeneration is poorly understood. Muscularis macrophages residing in close proximity to enteric ganglia maintain neuromuscular function via direct crosstalk with enteric neurons and have been implicated in the pathogenesis of GI motility disorders like gastroparesis and postoperative ileus. The aim of this study was to assess whether ageing causes alterations in macrophage phenotype that contributes to age-related degeneration of the ENS.

Design Longitudinal muscle and myenteric plexus from small intestine of young, mid-aged and old mice were dissected and prepared for whole mount immunostaining, flow cytometry, Luminex immunoassays, western blot analysis, enteric neural stem cell (ENSC) isolation or conditioned media. Bone marrow derived macrophages were prepared and polarised to classic (M1) or alternative (M2) activation states. Markers for macrophage phenotype were measured using quantitative RT-PCR.

Results Ageing causes a shift in macrophage polarisation from anti-inflammatory ‘M2’ to proinflammatory ‘M1’ that is associated with a rise in cytokines and immune cells in the ENS. This phenotypic shift is associated with a neural response to inflammatory signals, increase in apoptosis and loss of enteric neurons and ENSCs, and delayed intestinal transit. An age-dependent decrease in expression of the transcription factor FoxO3, a known longevity gene, contributes to the loss of anti-inflammatory behaviour in macrophages of old mice, and FoxO3-deficient mice demonstrate signs of premature ageing of the ENS.

Conclusions A shift by macrophages towards a proinflammatory phenotype with ageing causes inflammation-mediated degeneration of the ENS.

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Footnotes

  • Contributors LB: designing research studies, conducting experiments, acquiring data, analysing data and writing manuscript; LN and JG: conducting experiments; SK and PJP: experimental design; AH: designing research studies, analysing data, overall guidance and writing manuscript.

  • Funding Supported by NIH grants AG045098 (LB), DK103966 (LB) and AG049622 (AH).

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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