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Original article
Favouring modulation of circulating lipoproteins and lipid loading capacity by direct antiviral agents grazoprevir/elbasvir or ledipasvir/sofosbuvir treatment against chronic HCV infection
  1. Hung-Yu Sun1,
  2. Pin-Nan Cheng2,
  3. Chiung-Ying Tseng1,
  4. Wei-Jen Tsai1,
  5. Yen-Cheng Chiu2,
  6. Kung-Chia Young1,3,4
  1. 1Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
  2. 2Department of Internal Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
  3. 3Center of Infectious Disease and Signaling Research, College of Medicine, National Cheng Kung University, Tainan, Taiwan
  4. 4Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan
  1. Correspondence to Dr Kung-Chia Young, No. 1 University Rd., Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan ; t7908077{at}mail.ncku.edu.tw

Abstract

Objective Lipid homoeostasis is disturbed in patients with HCV infection. Direct-acting antiviral agent (DAA) treatment eradicates chronic HCV viraemia, but the dynamics of lipid components remain elusive. This study investigates the clinical manifestation and mechanistic relevance of plasma triglyceride (TG), cholesterol (Chol), lipoproteins and apolipoproteins (apos) after DAA treatment.

Design Twenty-four patients with chronic genotype 1 (GT1) HCV treated with elbasvir/grazoprevir or ledipasvir/sofosbuvir for 12 weeks, and followed-up thereafter, were recruited. Their TG, Chol, apoAI and apoB levels were quantified in plasma samples and individually fractionated lipoprotein of various classes. Liver fibrosis was evaluated using the FIB-4 Score. The TG and Chol loading capacities were calculated with normalisation to apoB, which represents per very low density lipoprotein (VLDL) and LDL particle unit

Results DAA treatment achieved a sustained virological response rate of 91.7% and reduced the FIB-4 Score. Relative to the baseline, the plasma TG level was reduced but the Chol level increased gradually. Plasma apoB levels and apoB/apoAI ratio were transiently downregulated as early as the first 4 weeks of treatment. The TG and Chol loading capacities in VLDL were elevated by ~20% during the period of DAA treatment and had steadily increased by 100% at follow-up. Furthermore, the TG-to-Chol ratio in VLDL was increased, while the ratio in LDL was reduced, indicating an efficient catabolism.

Conclusion The DAA treatment of patients with chronic hepatitis C might lead to efficient HCV eradication and hepatic improvement concomitantly evolving with favouring lipoprotein/apo metabolisms.

  • direct-acting antiviral agent
  • hepatitis C virus
  • lipoprotein metabolism
  • apolipoprotein

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Footnotes

  • Contributors H-YS: Study concept and design; acquisition of data; analysis and interpretation of data; drafting of the manuscript; P-NC: Clinical sample collection; analysis and interpretation of data; drafting of the manuscript; C-YT and W-JT: Perform experiments; Y-CC: Clinical sample collection; K-CY: Study concept and design; analysis and interpretation of data; drafting of the manuscript; critical revision of the manuscript for important intellectual content; obtained funding; study supervision.

  • Funding The Ministry of Science and Technology of the Republic of China, Taiwan, supported the research under Contract No. MOST 102–2628-B-006–006-MY3 and 105–2811-B-006–031-MY3.

  • Competing interests None declared.

  • Patient consent Detail has been removed from these case descriptions to ensure anonymity. The editors and reviewers have seen the detailed information available and are satisfied that the information backs up the case the authors are making.

  • Ethics approval Institutional Review Board of National Cheng Kung University Hospital.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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