Abstract
A simple filter-paper method forin vivo assessment of cytokines in intestinal mucosa of patients with ulcerative colitis (UC) was developed and evaluated. Twenty-eight patients were included. Twenty-three patients had ulcerative colitis (UC) and five irritable bowel syndrome (IBS). Inflammation was assessed endoscopically. Through a rectoscope, filter paper was applied to the macroscopically most inflamed area of the rectal mucosa until soaked. The filter paper was transferred to a buffer solution, and IL-1β and IL-1ra were assessed using ELISA. Positive correlations between endoscopic grading and ln(IL-1β) (P<0.0001) and ln(IL-1ra) (P<0.001) and a negative correlation between endoscopic grading and ln(IL-1ra/IL-1β) (P<0.02) were found. In measurements during and after a flare-up no significant change in IL-1ra but a significant decrease in IL-1β was detected, which is in agreement with investigations on biopsies. In conclusion, the filter-paper technique is an easily applicable, low-risk method that provides a means of monitoring cytokinesin vivo in UC.
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This work was sponsored by P. Carl Petersens fond and Fonden af 1860.
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Hendel, J., Nielsen, O.H., Madsen, S. et al. A simple filter-paper technique allows detection of mucosal cytokine levelsin vivo in ulcerative colitis. Digest Dis Sci 41, 1775–1779 (1996). https://doi.org/10.1007/BF02088744
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DOI: https://doi.org/10.1007/BF02088744