A monoclonal antibody specific for the hepatocyte receptor binding site on hepatitis B virus☆
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Cited by (34)
A monoclonal antibody specific to the non-epitope region of hepatitis B virus preS1 contributes to more effective HBV detection
2013, Clinical BiochemistryCitation Excerpt :The N-terminal region of preS1 is thought to mediate the attachment of the virus to cells and to possess neutralizing epitopes [4]. The aa 21–47 region of preS1 has been recognized as the epitope region based on the following observations: 1) the majority of the established preS1-specific mAbs were confirmed to bind to this region [18]; 2) several mAbs based on this region were proven to neutralize viral activity and block the entrance of virus into host cells [6–9]; and 3) all of the commercially available mAbs for clinical HBV LHBS detection are specific to this region [12,19]. The presence of an antibody specific to aa 21–47 of preS1 is also considered to be a marker of improved health in hepatitis B patients [20].
Hepatocellular Carcinoma Is Associated With an Increased Risk of Hepatitis B Virus Recurrence After Liver Transplantation
2008, GastroenterologyCitation Excerpt :Immunostaining was performed on sections of fixed liver tumor and nontumor samples using a commercial 3-step streptavidin-biotin technique, according to the manufacturer's instructions (Ventana Medical Systems, Strasbourg, France). The primary monoclonal antibodies employed were anti-HBsAg, anti-HBcAg (Dako, Copenhagen, Denmark), and anti-pre-S1 (kindly provided by Dr M. A. Petit).31 S gene sequences were obtained from sera and liver tissue using the following forward and reverse primers: 5′-TGGYTATCGCTGGATGTGTC-3′ and 5′-CCCAAAAGACCCACAATTC-3′.
Mapping of a Conformational Epitope Shared between E1 and E2 on the Serum-derived Human Hepatitis C Virus Envelope
2003, Journal of Biological ChemistryCitation Excerpt :The results were considered as positive when superior to the cutoff, corresponding to the mean of negative controls multiplied by 2.1. Western Immunoblot Experiments—The untreated HCV-enriched pellet (HCV-L) was used as an antigenic probe (12, 13) at concentrations from 0.1 to 1 mg/ml. The antigen was subjected to SDS-PAGE on 12.5% gels under reducing or nonreducing conditions (2% SDS ± 5% β-mercaptoethanol).
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This work was supported by research grants from INSERM.