Alimentary TractAntibiotic therapy attenuates colitis in interleukin 10 gene–deficient mice☆,☆☆
Section snippets
Animals
Homozygous IL-10 gene–deficient mice, generated on a 129 Sv/Ev background, and 129 Sv/Ev controls were housed behind a barrier under specific pathogen–free conditions. All provisions for the facility were autoclaved. Nonautoclavable supplies were sprayed with disinfectant and introduced through an HEPA-filtered air lock. Mice were housed in micro-isolator cages with tight-fitting lids containing a spun-polyester fiber filter. In sentinel Balb/c mice, bacterial cultures, parasitological
Animal profile
There was no difference in the average number of pups born to control and IL-10 gene–deficient mothers (data not shown). At 2 weeks of age, IL-10 gene–deficient pups had body weights similar to those of controls (control,10.4 ± 0.3 g; IL-10 gene–deficient, 8.5 ± 0.6 g). However, by 4 weeks of age, IL-10 gene–deficient mice had significantly lower body weights than their age-matched controls (control, 20.7 ± 1.9 g; IL-10 gene–deficient, 15.0 ± 0.4 g; P < 0.05); this difference persisted
Discussion
There is increasing evidence from both clinical studies and animal models to incriminate luminal bacteria or bacterial products in Crohn's disease initiation and progression. The spontaneous colitis that develops in transgenic HLA-B27/β2-microglobulin rats,13 IL-2,16 IL-10,4 and T-cell receptor–deficient mice17 requires the presence of luminal bacteria; animals raised under germfree conditions generally do not develop colitis in these genetically engineered rodent models. Furthermore, in
Acknowledgements
The authors thank the University of Alberta Hospital Microbiology Laboratory for assistance in bacterial culture and Schering Canada Inc. for support of the interleukin 10 gene–deficient mouse colony.
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2020, Cellular and Molecular Gastroenterology and HepatologyCitation Excerpt :A combination of ciprofloxacin (200 mg/L) and metronidazole (600 mg/L) was added to drinking water of CD4-Cre+/Tg Mettl14FL/FL conditional knockout mice. The antibiotics doses have previously been shown to attenuate experimental colitis in mice.33 Treg cells were isolated by FACS sorting from Foxp3YFP mice based on YFP expression.
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2019, Seminars in ImmunologyAntibiotics exacerbated colitis by affecting the microbiota, Treg cells and SCFAs in IL10-deficient mice
2019, Biomedicine and PharmacotherapyCitation Excerpt :However, in the IL10-/- mice treated with antibiotics, the mean acetate content of the colon was 1142.8 mg/L. Many studies have shown that antibiotic therapy may attenuate colitis in IL10−/− mice [5–7]. However, all these results have shown that antibiotics were more effective for prevention than for treatment, especially for the treatment of IL10−/− mice with established colitis [5–7].
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Address requests for reprints to: Richard N. Fedorak, M.D., Division of Gastroenterology, University of Alberta, 519 Newton Building, Edmonton, Alberta, Canada T6G 2C2. e-mail: [email protected]; fax: (780) 407-3744.
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Supported by Crohn's and Colitis Foundation of Canada and the Alberta Heritage Foundation for Medical Research.